In the Soap OperaPassions, Ethan was attacked by a demon from the Bennett's closet to Hell. The shriek he let out was very girlish. So girlish in fact, many people commented on it on the internet and one of this Troper's co-workers said, "Not even my three-year-old girl screams like that!"
In Castle, the title character gives one when a dead body tumbles out of the fridge he opens. Naturally, he attempts to protect his dignity by covering it up as a cough. Surprisingly, no one comments on it.
Happens fairly often, actually, like when he visits Beckett at home and, due to having a stalker at the time, she opens the door with her gun pointed at him.
Frasier. Both Frasier and Niles scream like a girl.
In the episode "Boo!":
Frasier: That is not funny! You don't sneak up on someone and startle them! Martin: First of all, it's very funny. And secondly, I only did it to prove a point. Ronee: And you called it: he does scream like a woman. Frasier: I do not scream like a woman. It was a manly throaty wail. (Niles comes in the front door) Niles: Frasier, you may want to call security. As I got off the elevator, I heard a woman screaming hysterically.
In the episode "Don't Go Breaking My Heart", after a womanly high-pitched scream was heard.
Daphne: Are you all right? Niles: Yes. I just jumped into bed with your mother. Daphne: Oh, dear. No wonder she screamed. Niles: That wasn't her.
Friends. Ross screams like a little girl when Rachel and Phoebe come out of his apartment's curtains and shout danger to scare him.
On an early Ernie and Bert sketch on Sesame Street, after counting sheep and fire engines, the scanimate balloon pops which causes the bedroom to shake and scare Bert.
In the episode "War of the Coprophages", Mulder has a discussion with Scully regarding a childhood encounter with a praying mantis...
Mulder: I had a praying mantis epiphany, and as a result I screamed. No, not... not a girly scream, but the scream of someone being confronted by some before unknown monster that had no right existing on the same planet I inhabited. Scully: Mulder... are you sure it wasn't a girly scream?
In a later episode with the same writer ("Jose Chung's From Outer Space"), we get to hear Mulder's girly scream upon spotting "a real live dead alien body" — at least according to one version of the story.
In one episode of Saturday Night Live hosted by David Duchovny, the opening sketch is a parody of The X-Files that takes place backstage at SNL. At one point Duchovny hears a girly scream, runs toward it, and finds cast member Molly Shannon, thinking it was her. He finds out it was actually fellow cast member Chris Elliott who screamed.
In "Mr. Monk Goes Camping", when the eponymous obsessive detective encounters a bear, the previews feature actor Tony Shaloub's own scream, whereas the scream in the episode is overdubbed with a little girl's.
Barney has a couple of instances of this as well. A notable example is when he accidentally burns a hole through one of his ties.
Used numerous times on Psych. Someone even made a montage of it.
After Shawn and Gus are at a seance. A ghost appears, and then the lights cut back on. Shawn's love interest wonders where he and Gus went. Cut to the outside of the building, where Shawn and Gus are running for their car while screaming incomprehensibly, thus. What makes it funnier is that they're running out of a sorority, but none of the girls inside screamed.
Zack: I yelped! I didn't scream. Fisher: He screamed like Slutty Girl Number One in a cheesy horror flick.
In season 3 episode 5, Booth yelps in an extremely girly way not once but twice when confronted with a clown. This is one of the few times, if not the only time, the alpha-male-gets-things-done image of his gets shattered. Needless to say, it's hilarious.
Matthew Gray Gubler: So, we were filming the scene in which my character was being tortured. And in between takes, everyone was laughing, and I couldn't figure out why. And finally, at the end of one take, the director came up to me and said, "Okay, but can you try screaming in a way that does NOT sound like a little girl?"
An actor in The Bill had a scene where he fell off a platform while chasing a criminal in an Abandoned Warehouse. He mentioned that his scream as he did this stunt had been too high pitched, so they had to dub a more 'manly' scream on afterwards.
In Community, when the going gets tough Troy has a tendency to regress into the personality of a six-year-old girl, completely with girly screams and panicky dancing.
An episode of Mighty Ships set on the largest carrier in the world, the USS Nimitz, showed a rookie pilot getting his callsign, which is usually done by consensus of other pilots in the rookie's flight group. This particular pilot had made no mistakes even when landing on a carrier at night, but when catapulted off for the exercise had, in his own words, screamed like a little girl. Another pilot noted that "screams like a girl" could be acronymed into "slag", which is also a British slang term meaning, roughly, slut, and (though this wasn't mentioned on the show) is originally an industrial term for waste product from smelting. Congratulations, Lieutenant Slag....
A shrill scream rings through the corridors of Flatpoint High in Strangers with Candy. Mr Noblet instantly knows who's in trouble:
"My God! That's Jellineck!"
Nick in New Girl screams like a girl in a Halloween episode.