The first half of the Father/Son Basketball game, with the sons pulling out the Harlem Globetrotters play book - Of note, is Dan telling Nathan not to pull that crap with him, and Nathan doing it anyway.
Episode 1x06, "Every Night Is Another Story"
Stoned Brooke.
Episode 2x01, "The Desperate Kingdom of Love"
Haley's parents asking if Nathan entered the James house through the window. Especially when Haley's father sarcastically gives Nathan a So Proud of You response.
"I think I see where this is going, Nathan. But we can't adopt you. I mean, if we do, the making-out with Haley is going to have to stop. [Beat] At least in public, anyway."
Episode 2x06, "We Might As Well Be Strangers"
Lucas busting out the "And what time do you call this?" routine on Karen, before revealing he's only been home for 5 minutes.
Even funnier when you realise that it's the exact same dialogue from when Karen caught Lucas sneaking in after curfew in Season 1.
Episode 2x15, "Unopened Letter to the World"
Dan in church.
Jules: You couldn't turn your cellphone off in a church?
Dan: Might be God.
Episode 2x19, "I'm Wide Awake, It's Morning"
The women of Tree Hill at Tric.
Deb: I miss Nathan.
Karen: I miss Keith.
Peyton: I miss Jake.
Brooke: I don't really miss Felix at all... Or my parents that much... But I miss my money.
Episode 3x15, "Just Watch The Fireworks"
Pete Wentz hitting on Peyton.
Pete: So... Be right back, I'm gonna go save the world.
[Beat. Pete leaves, Patrick Stump (FOB lead singer) walks up.]
Patrick: Did he just roll out "Gotta go save the world"?
The shot panning across the girls applying their make-up in the bathroom mirror. Well, the girls and Pete Wentz.
Episode 4x03, "Good News for People Who Love Bad News"
After Nathan gets an award for Valor, Rachel runs up & kisses him.
Haley: Stay the hell away from my husband.
Rachel: What? I was just thanking him.
Haley: Well, your thanks send people to the Free Clinic.
Peyton: Ha!
Brooke: What are you laughing at? Your love sends people to the morgue.
Peyton: Well then, I love you, Brooke.
After sitting out the first half, Skills rants to Nathan about Whitey holding a brother down, and that someone should tell Whitey that they don't call him Skills because he's good at picking splinters out of his ass. Nathan simply replies "Okay." and turns his head slightly to the left & repeats that to Whitey.
Deb returning from rehab to find the house littered with empty beer, whiskey & vodka bottles from the party Nathan had thrown the previous night... And thinking that it's her mess, apologises for it. Nathan simply responds with a simple "Whatever, Mom. Just clean it up."
"4 years of high school, and zero girlfriends for Junk & Fergie."
Episode 5x01, "4 Years, 6 Months, 2 Days"
Jamie & Lucas's first scene together.
Lucas: J-Luke! Are you in high school already?
Jamie: No, I'm only 4.
"Junk & Fergie play video games, drink beer & eat pizza; I'm Mouth McFadden, and I'm still unemployed."
Haley picking Jamie up after spending the day with Lucas & the River Court guys.
Jamie: Bye Uncle Lucas, I have to go home now. Besides, you should be writing.
[Haley & the guys burst into laughter]
Lucas: I don't wanna hear it.
Episode 5x02, "Racing Like A Pro"
"For evey step you take, we kiss."
"Have you ever had a wine hangover?" "I have a fluffy earred bunny named Chester."
The sponsors on Jamie's racing uniform.
Jamie: Peyton Sawyer - Unemployed!
Episode 5x11, "You're Gonna Need Someone On Your Side"
Owen bringing Chase as a blind date for Peyton to the joint bachelor/bachelorette party, completely oblivious to his history with Brooke. On the car ride to the Scott house, Chase asks if he remembers the girl he told Owen about when he first moved in with him, specifically her name. You can see the cogs turning in Owen's brain, before he suddenly realises it's the same Brooke, much to the amusement of Chase, Brooke & Peyton. Later, Owen comments to Chase that one of them should have realised, since he said Brooke & Chase said Brooke... Only for Chase to point out that he said "Brooke who started a clothing line".
Skills being utterly terrified of Chester.
Episode 5x12, "Hundred"
Skills tailgating before Lucas's wedding to Lindsey, before going on to encourage Peyton to interrupt the wedding & declare her love for Lucas, before musing that maybe Lucas will say the wrong name at the altar, with Brooke commenting that none of that will happen because This Is Reality. Peyton then follows up by saying she'll just get drunk at the reception & have sex with some random guy, if that's okay with Skills... Who promptly starts hitting on Peyton.
And when Peyton does stand up to declare her feelings for Lucas, Skills happily declares "That's what I'm talking about!". Of course, it's swiftly revealed to just be a dream.
Brooke finally revealing why she came back to Tree Hill.
Lucas: The way I see, Skills'll be better father than Dan ever was.
Episode 6x16, "Screenwriter's Blues"
Mia & Peyton's attempt at making a crib.
Peyton "auditioning" for the role of Peyton in "An Unkindness of Ravens" & being turned away by Reece for being too old. Especially in a meta-sense, when you remember the Dawson Casting that the characters were originally subject to, and that James Vàn Der Beek was in the trope naming show.
Episode 6x21, "A Kiss to Build a Dream On"
"Welcome to Tree Hill; try to find a boy who didn't lose his virginity to Brooke Davis."
The "Stay out of it, Nick Lachey" scene at TRIC.
Episode 6x23, "Forever And Almost Always",
Lucas and Peyton's wedding has arrived and Brooke and Julian, recently broken up, have attended separately. Julian has brought "movie Brooke" as his date and Brooke has brought... Nick Lachey. At the reception, to piss off Nick, Julian says the quote mentioned below, earning himself a punch in the face in the process:
Julian: It's, like, crazy hot in here; it must be, like... 98 degrees...
Jump Cut to Julian putting ice on his eye, and asking Peyton why guys in Tree Hill keep punching him, and Peyton pointing out that Julian was asking for it by mocking Nick's boyband... Which Julian responds to with a simple "Well, yeah!" in a tone that basically sounds like he's really saying "Well, of course I was going to mock his boyband! What did anybody expect?" & Peyton responding to that by reminding Julian that he likes 98 Degrees.
Brooke bursting into the tent where Haley & Peyton are getting ready.
Brooke: I am SO pissed right now, that whore stole my man!
Quinn & Haley arguing about whether everyone is turning into vampires or zombies, despite Haley already being bitten.
Brooke & Julian fighting zombies, only for Brooke to get distracted by one of the zombies knocking the cover off her wedding gown, and Quinn's frustration that everyone keeps calling the zombies vampires.
Haley's costume - a pregnant cheerleader, or as she puts it, "Me in high school."
Episode 8x07, "Luck Be A Lady"
If ever proof was needed that Clay Evans is an Adult Child, the opening scene of this episode should be cited, with Nathan walking in on Clay dancing & lip syncing to "Get Down Tonight" by KC & the Sunshine Band.
Clay: Quinn's out of town, what'd you expect?
Nathan: Literally anything else.
Clay: Yeah... Hey, I got an interesting phone call this morning.
Nathan: Yeah. Sorry, about that. Jamie's been calling eveybody about this frog he found.
Clay: Nah it— Jamie found a frog? That's awesome.
Episode 8x13, "The Other Half Of Me"
Skills being Genre Savvy about Tree Hill wedding days, culminating with him bolting out of his seat & yelling "Nobody say NOTHING!" when the priest tells everyone present to Speak Now or Forever Hold Your Peace.
Nathan: Hey, little boy - Can I kidnap you? Oh wait, you've already been kidnapped.
Jamie: So have you.
Nathan: Exactly. We go to say goodnight, you're missing, your mom starts freaking out - You realise we're the Scott family, right?
...
Nathan: I'll have to figure out how to talk your mom into not grounding you.
Jamie: Oh! Maybe we could say I was kidnapped again!
Nathan: Eh, it's a little played out.
"You named your guitar after my wife?"
Chris Keller & Clay both having the immediate reaction to slap Chase after tasting one of his drinks for the first time. Not to mention Mouth remembering how bad Chase's drinks are.
