Watching the High Overseer take the Captain to see a portrait of himself, only for him to realize that it...uh...went missing
In one area, you can find two bodies, one with a sword sticking out of his chest, sitting at a card table. Looking at the score card, you'll notice the score sits at 130 to 3 when the writing is abruptly cut off.
Finding Piero peeking on Calista taking a bath. He tries to make an excuse and then admits that he's just rather lonely.
And then Corvo walking into the bath and asking if he can join her.
Should Corvo leap into the bath with her, she panics and you get a Non-Standard Game Over citing that the Loyalists disbanded due to "irreconcilable hostilities."
Some of the Enemy Chatter is amusing, like a guard telling one of his fellows that he needs a bath, or two Bottle Street goons exchanging dirty limericks at the Dunwall Distillery.
Guard 1: So how old's your sister again?
Guard 2: I don't need shit from you.
Miss White's reaction to Corvo's "costume" in Lady Boyle's Last Party:
Also, Esma Boyle's reaction to Corvo telling her that someone here to kill her. Please note that none of her dialogue is in any way sarcastic. She is completely serious.
Esma: Oh, but this is perfect! I love it! What happens next? Are you going to tie me up?
Corvo's first potential answer is "Never mind." However, if he chooses that, she tells him not to stop and implores him to come with her.
Her journal specifically says that she's lonely and is willing to have sex with the first man who asks and then the second man as well. It all gives a really kinky vibe to the creepy stalker/assassin angles.
Know that pesky guard that won't let you upstairs? Blink past him. He'll stare in confusion at the empty space and never realize where you actually went.
The same guard, I waited long enough and he proceeds to wander away, down into the kitchen, leaving the stairs open.
You can even use your powers to possess him! Of course he's tied down and blindfolded so you can't see or do anything, but still.
You can also steal his invitation to Lady Boyle's party from his apartment. When you turn up to the party two missions later and flash his invitation, the guards say they ejected a man earlier for "impersonating" you. Naturally, you waltz in like you own the place - and leave behind the actual Bunting, who's sulking near the fence.
If you choose to go with the nonlethal route in the duel with Shaw, the two men helping still react as if he's dead... Despite the fact that he can be clearly heard snoring. Not exactly students of Natural Philosophy, are they?
Piero and Sokolov are long-running rivals, insulting each other's plague remedies, feuding over efforts to utilize whale-oil, and generally having a good old scientist dustup. Finally, towards the end of the game, you encounter them both in Piero's workshop... amicably discussing their respective plague countermeasures and talking about how combining their approaches has real merit.
Even better, they are doing this as the City Watch lays siege to the workshop. They're both so utterly enthusiastic and excited about their mutual brilliance and love for SCIENCE! that you'd be forgiven for thinking they're not even aware that exploding bolts are pelting the workshop until you drop down to find them hiding under desks... while having their conversation.
Speaking of these two, pay attention during the low Chaos ending: when the Outsider shows the two with the cure remedy, Sokolov can be seen forcing a restrained man to drink some kind of...concoction.
In this case, it's implied that Sokolov and Piero together discovered a cure to the plague, and he's actually treating a Weeper. But both of them look happier at what they've created than the lives they've saved.
And even earlier than that: after kidnapping Sokolov and preparing to interrogate him about the identity of Lady Boyle, Piero will give Corvo some advice on dealing with the man.
Later, the machine disappears from Havelock's room and winds up in Samuel's possession, and in the Boatman's first recording he mentions that he fished it out of the river. Guess the Admiral's no good with machines.
When you go back to the Pub after being betrayed by the loyalists, one of the first mission hints you get is "Many city watchmen remain standing." If you activate the improved arc-pylon invented by Piero and Solokov,note which can either render every guard in the vicinity unconscious or burn them to ashes you get another hint: "Few city watchmen remain standing."
In "The Knife of Dunwall," Daud finally finds a guard who actually did get his own squad after what happened last night, and another who's sick of his colleague talking about whisky and cigars.
City Watchman: Sir, I just wanted to say... congratulations on getting your own squad.
Rothwild Butcher: Do you ever think about anything other than whiskey and cigars?
Other Butcher: 'Course.
Lurk's deadpan snark. The mask helps.
There's a man in a boat keeping an eye on the slaughterhouse. If I weren't such a pure and innocent soul, I'd say he's up to no good.
As for lockpicks? A fable. I've never known such a thing to truly exist. Have you?
There's a point in the flooded district where Corvo can come across a pair of assassins, one of whom is training the other. The student is told to cross from one side of the room - the side Corvo is on - to the other, without being seen. Corvo can then follow the student through the course, without being seen by either of the assassins.
The fact that Piero and Trimble where such horrible shots that their Duel to the Death had to be turned into a coin toss.
Ah, Lizzie Stride. Specifically, Lizzie Stride reclaiming her gang after Wakefield sold her out.
Lizzie: I forgive you! All of you! I'm filled with love. But, the following people each owe me a finger: Logan, Douglas, Bang-Bang, Ferris, Pigface, the Bakers and Annabelle.
Lizzie:Two from you, Annabelle. Have a good night!
In Brigmore Witches upon catching a Bone Charm from a clogged toilet. Daud's reaction is about what you'd expect.
Daud:(disgusted) This better be worth it.
The non-lethal takedown of Barrister Timsh in The Knife of Dunwall DLC is pure hilarity and poetic justice. Barrister Timsh, the target of that level, amassed great wealth by accusing people of harboring the plague and confiscating their property for Lord Regent. If Daud tosses a smelly bundle into the ventilation system, the visiting Watch Officer complains that Timsh's building smells like a Weeper den, and Timsh hands him the document that he thinks gives him immunity from confiscation of property for the Lord Regent. Instead, the document he hands over is a falsified letter, claiming that his property was to be confiscated for the Lord Regent a month ago. Timsh is so shocked that he actually faints, and the guard won't even carry him off because Timsh might have the plague.Schadenfreude doesn't begin to describe it.
Taking out Delilah Copperspoon non-lethally is positively hilarious, particularly if she's already unconscious, because Daud will complete the ritual for her. He throws her on the altar, and she gets sucked into the Void. She grabs on to him, and he pimp-slaps her before she gets sucked in. Even if she completes the ritual, her final fate is infinitely amusing.