Actor Allusion: In the third part of "Once Upon a Time on Mars", the Biker Mice get into an argument on what kind of maneuver to use to destroy the Plutarkian Tug Transformer, all of their suggestions being allusions to their voice actors. Vinnie (voiced by Ian Ziering) suggests the Zip-Zapping Ziering Zero, Modo (voiced by Dorian Harewood) suggests the Harewood Crooked 10, and Throttle (voiced by Rob Paulsen) insists on using the Rob-bobbin' Paulsen Doggle 2.
Cross-Dressing Voices: Susan Silo as Dr. Karbunkle, and Leeza Miller-McGee (Charley Davidson) as Modo's nephew Rimfire. Only once, though.
While it finally aired in America on 4Kids in 2008, seven episodes never got a chance to air before the show was pulled from the schedule ("Desperado", "Cat and Mouse", "First Mice on the Moon", "Once Upon a Time on Earth" parts 1-3, and the Grand Finale "Turf Wars").
DVD releases have only been available in the United Kingdom, Australia, New Zealand, and Bulgaria.
The Other Darrin: While the 2006 revival had some Role Reprisal from members of the original cast, like having Rob Paulsen, Dorian Harewood, and Ian Ziering back as the titular Biker Mice, most actors had to be replaced.
Lisa Zane replaces Leeza Miller-McGee as Charley Davidson.
While Peter Strauss reprises his role as Stoker for five episodes, the rest of the series has him replaced by Jim Ward.
Jess Harnell replaces Brad Garrett as Greasepit, Brian Austin-Green as Rimfire, Stu Rosen as the Pit Boss, and Jeff Bennett as Lord Camembert.
Production Posse: Several actors from Beverly Hills 90210 have roles in the series. Ian Ziering (Steve Sanders) plays Vinnie, Luke Perry (Dylan McKay) voices Napoleon Brie, Brain Austin Green (David Silver) voices Modo's nephew Rimfire, and Jason Priestley (Brandon Walsh) guest-starred in two episodes as Jack McCyber.
Short Run in Peru: The revival initially aired from 2006 to 2007 on the British television channel CITV. The series did eventually air in America on 4Kids in 2008, but was pulled from the schedule before every episode had a chance to air. While the revival was said to resume airing on TheCW4Kids in fall 2009, it never came to pass.
Word of God: Original series co-creator Tom Tataranowicz has stated that he believes none of the Martian mice have last names and have avoided them altogether because surnames were considered a liability and the Martian mice didn't need them because they could identify their relatives with no problems, though he has acknowledged that Rick Ungar may have a different idea behind the Biker Mice's First-Name Basis.