In "The Motor City Maniac", Vinnie comments that he finds Napoleon Brie familiar for some reason, with Brie responding by suggesting that they may have gone to high school together. This is a reference to the fact that their voice actors Ian Ziering and Luke Perry both played characters on the teen drama Beverly Hills, 90210.
In the third part of "Once Upon a Time on Mars", the Biker Mice get into an argument on what kind of maneuver to use to destroy the Plutarkian Tug Transformer, all of their suggestions being allusions to their voice actors. Vinnie (voiced by Ian Ziering) suggests the Zip-Zapping Ziering Zero, Modo (voiced by Dorian Harewood) suggests the Harewood Crooked 10, and Throttle (voiced by Rob Paulsen) insists on using the Rob-bobbin' Paulsen Doggle 2.
While it finally aired in America on 4Kids in 2008, seven episodes never got a chance to air before the show was pulled from the schedule ("Desperado", "Cat and Mouse", "First Mice on the Moon", "Once Upon a Time on Earth" parts 1-3, and the Grand Finale "Turf Wars").
DVD releases have only been available in the United Kingdom, Australia, New Zealand, and Bulgaria.
The Other Darrin: While the 2006 revival had some Role Reprisals from members of the original cast, like having Rob Paulsen, Dorian Harewood, and Ian Ziering back as the titular Biker Mice, most actors had to be replaced.
Lisa Zane replaces Leeza Miller-McGee as Charley Davidson.
While Peter Strauss reprises his role as Stoker for five episodes, the rest of the series has him replaced by Jim Ward.
Production Posse: Several actors from Beverly Hills, 90210 have roles in the series. Ian Ziering (Steve Sanders) plays Vinnie, Luke Perry (Dylan McKay) voices Napoleon Brie, Brain Austin Green (David Silver) voices Modo's nephew Rimfire, and Jason Priestley (Brandon Walsh) guest-starred in two episodes as Jack McCyber.
Short Run in Peru: The revival initially aired from 2006 to 2007 on the British television channel CITV. The series did eventually air in America on 4Kids in 2008, but was pulled from the schedule before every episode had a chance to air. While the revival was said to resume airing on TheCW4Kids in fall 2009, it never came to pass.
William Morgan Sheppard voices both Lawrence Limburger and the Sand Raiders' leader Slobber the Mutt.
Jim Cummings voices both Saddle Sore and Deathmaster in "So Life Like" as well as the Plutarkians Gerald Gruyere and Gutama Gouda in "Diet of Worms".
Word of God: Original series co-creator Tom Tataranowicz has stated that he believes none of the Martian mice have last names and have avoided them altogether because surnames were considered a liability and the Martian mice didn't need them because they could identify their relatives with no problems, though he has acknowledged that series creator Rick Ungar may have a different idea behind the Biker Mice's First-Name Basis.