Barbie Doll Anatomy: Mocked by Mike and the bots during the skinny-dipping scene. The girls were meant to be nude in-universe, but since the actresses were wearing Nude-Colored Clothing, it had this effect.
Crow: (As the girls) We're nude and we have no nipples and we're uniformly colored!
Call-Back: When Mike and the bots are joking about the scientist being caught in a web "in the midst of a cheerleading routine", Crow mentions "MRxL."
Crow: Hah. He saw an air molecule that respects women.
Historical In-Joke: When the girls try and fail to flag down a passing cruise ship, Mike remarks "Damn Californian, never helps anyone!" The Californian was a ship that was in the vicinity of the Titanic when it sank but didn't render aid for reasons that remain vague. May also count as a Genius Bonus.
The Internet Is for Porn: During one of the cat fights, Crow notes that usually you have to go online to find this sort of thing, namely "Naked Wrestling Giantesses dot com".
Ironic Echo: Mike and the bots tend to dramatically proclaim "THE HORRORS OF SPIDER ISLAND!"—shifting the emphasis between horrors and spider — whenever the movie ratchets up the Fanservice.
This is actually a Call-Back (way back) to Manos: The Hands of Fate, where Joel and the Bots spent the first half of the movie doing the same dramatic title drops whenever nothing was happening onscreen (which, again, was pretty much the first half of the movie... And maybe a good deal of the second half, too).
Gary: There you are, Georgia. Servo: Just north of Florida. Mike: D'oh!
Never My Fault: After Gary inexplicably blames his kissing another woman on the heat.
Servo: "Yes, I've been unfaithful, but it was like 87 degrees." Crow: "It's also kinda windy. That, too. And high humidity..." Servo: "You see, if it was in the low '70s, I would never have jammed my tongue down her throat."
Plot Armor: When the plane hits the water at a 90 degree angle and explodes, Servo sarcastically quips, "I'm sure they're all fine."
Power Creep, Power Seep: When Pearl tells Observer that he could have teleported the castle instead of an arduous and expensive move, he huffs, "I'm not that omnipotent, Pearl!"
Rich in Dollars, Poor in Sense: Pearl spent several million dollars to move the castle in order to save $40 a year on Bobo's ape license. And then had to move it back because she couldn't be bothered to leave her trash out on the curb like a normal person.
Running Gag: Twice during the film, Tom Servo faints from the fanservice/cat fights.
Something Completely Different: Unlike season 8 when Pearl and co. were always changing locations, Castle Forrester was always in the same place until this episode, when it abruptly moved to a suburban neighborhood. The reason? The mountaintop where it used to be had the gall to charge Pearl $50 for an ape license. However, by episode's end Castle Forrester was back on the mountain, since Pearl discovered she couldn't throw her trash out the window.
Soundtrack Dissonance: Referenced; the film often plays jazzy music during what's supposed to be a horror or action sequence.
Mike: (chuckles) You know, the Dukes of Dixieland also did the Psycho soundtrack.
Earlier, the skinnydipping scene is accompanied by what sounds like a busy '50s stock piece.
Servo: Ah yes, the hustle and bustle of city life.
The Talk: Pearl gives one to Bobo after he discovers some condoms but mistakes them for balloons. She does this off-camera, telling Mike and the Bots they're too young for it. We can't hear what Pearl says, but Bobo makes Squick noises.