Black Widow: Pearl has racked up quite the collection of dead husbands and fiancés.
Pearl: Sit back down or I'll marry you!
Body Horror: After Pearl sprays the SOL with poison, Crow grows extra limbs, Tom's hands grow huge, and Mike grows an extra Tom Servo out his back. They all return to normal once Pearl sprays the SOL with an antidote.
In the rough cut, the guys reference the "Owner of a Lonely Heart" joke from Final Justice; at one point, Patrick Brantseg starts playing the song, and the guys remark that they have to listen at least to the orchestra hit.
Continuity Nod: A handful of references are made to Werewolf, probably due to the stupidly similar plot seen once this movie really gets rolling.
Historical In-Joke: The entire spraying the S.O.L. with mutagens is a reference to Operation LAC when the CIA sprayed zinc cadmium sulfide over Minnesota, among other states, just to see what would happen (answer: severe respiratory problems.) The CIA later apologized in the 1990's, pretty much in the flat way Pearl did, with the same excuses Pearl gave. BBI mentioned it in The Amazing Colossal Guide. They, too, were not amused.
Hurricane of Euphemisms: In the rough cut, the guys remark on the fact that Coily could be interpreted in...dirtier ways. This leads to them coming up with many names for poop sprites like "Fecey" and "Stooly".
Porn Stache: Mike attempts to see how one looks on him and asks what Pearl's opinion is. She likes it, mildly. Crow goes for an even bigger one, which makes her quite smitten with him. Servo takes his Up to Eleven, but it's just so big that he falls over.
Precision F-Strike: In the rough cut version, during one later scene, both Crow and Tom get in on this version of one of the episode's running gags.