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Nightmare Fuel: Sonic the Hedgehog (2006)
Mephiles the Dark's introduction from Sonic the Hedgehog 2006: He slouches over and walks as though a puppet on strings with his creepy staring eyes, able to talk without moving his mouth, all with this music playing.
At the start of the last story, Mephiles appears out of nowhere and instantly kills Sonic. Even though Sonic comes back to life eventually, it still happened.