While the punishments from the kids onto the Eds usually leave them cowering in fear, the fact that all three of them are panicking and trying to run for their lives, visibly scared the whole time at the start of The Movie is unsettling; especially with the kids actually pissed off enough to try and murder them.
The kids's injuries are also pretty freaky. Jonny has a bear trap on his head and Rolf has most of his torso torn off! What in God's name did the Eds do?!
The scene where Eddy and Ed seemingly sink in quicksand/mud, even though it's only a prank, it was surprisingly close to an actual death scene.
Double D's reaction to them faking their deaths. He is extremely pissed at them for scaring him that much. Sure he got mad in the main show, but here he is legitimately pissed off at them to the point of which he;s willing to go back to the Cul-De-Sac rather than spend another second with Eddy and that he actually gets into a fight with Eddy over the scams, and the two actually physically nuke it out. What makes it worse is that Ed watches the entire scene and he's powerless to do anything about his two best friends fighting.
Eddy's brother in general, and particularly the smile and stare he makes (pictured above). It stretches to around half his face. Worse, he's an Ax-CrazyPsychopathic Manchild who loves torturing children, and the only one in the series that really deserves these tropes. His Evil Laugh when he twists Eddy's leg as well. It's not a comical laugh, but a realistic, twisted, demented laugh. Even the music that plays during the scene is pretty freaky too, as it is a sinister tune mixed with an electric guitar. Listen to it here.