- All of the "Are You Pondering What I'm Pondering?" gags. ALL OF THEM!
- "One is a genius, the other's insane - and the other one's Larry."
- "Hi Brain. Do you know the lyrics to Muskrat Love?" "OCTOPUS!! HELLLP!!!"
- "The irony of it all, Pinky. Years of trying to take over the world, and all I had to do was say 'moo'."
- From the Halloween Special:
Brain: No! Pinky, you got chocolate on my Jack-o-lantronic transmitter!
Pinky: You got Jack-o-lantronic transmitter in my chocolate!
- Nine words: "Moo. We are a cow. Take us to China."
- The episode "But That's Not All, Folks!" where Pinky and The Brain transmit a live infomercial. Some of the skits they do are interesting.
- There is also the funniest variation of their closing line, after Pinky fouled up Brain's attempt to win a horse race:
The Brain: Quiet Pinky, I'm getting ready for tomorrow night.
Pinky: Why? What are we going to do tomorrow night?
The Brain: [Irritated] Guess!
Pinky: Oh, try to take over the world, right.
- In All You Need Is Narf'...
Yoyo Nono: You look so sad. I will sing you my happy song. EEEYYYAAAOOOEEE!!!
- Then a few more times after:
Yoyo Nono: I will sing my beautiful song. EEEYYYAAAOOOEEE!!!
Yoyo Nono: Oh, then I will sing my sad song. EEEYYYAAAOOOEEE!!!
Yoyo Nono: I will sing my love song. EEEYYYAAAOOOEEE!!!
And then again on the TV: EEEYYYAAAOOOEEE!!!
- "The Pinky POV".
- This basically explains how Pinky comes up with his non-sequitur answers to Brain's famous question.
- From That Smarts...
- Brain decides that Pinky has become smarter than he, and to balance the group he must become the dumb one. Cue his Oh, Crap! face right after he realizes Pinky has just done the same.
- This beautiful moment from Welcome To The Jungle:
Snowball: I have a joke for you, Pinky. Knock knock.
- From the Christmas special:
Brain: Just act natural.
Cue Pinky's flurry of verbal tics and laughs.
Brain: Not that natural.
- From Brinky...
Pinky: I'm a mommy! I'm a mommy!
- And then later, when Romy gets his own place and they visit.
- Romy sends Pinky and Brain a letter from his new home asking them not to try to find him but the envelope has his address written on it. Pinky still has no clue to his whereabouts.
- This exchange from "The Pinky Protocol":
Pinky: "I see an angel coming for us out of the sky."
- Pretty much anything Mr. Sultana says is hilarious, but this takes the cake:
- Are You Pondering What I'm Pondering? gets a serious lampshade:
Brain: Are you pondering what I'm pondering?
Pinky: Phew. Oh, I'd say the odds of that are terribly slim, Brain.
Pinky: I mean, really, when have I ever been pondering what you've been pondering?
Brain: To my knowledge, never.
Pinky: Exactly. So what are the chances that this time I'm pondering what you're pondering?
Brain: Next to nil.
Pinky: Well, that's exactly what I'm thinking, too.
Brain: (realizing) Therefore, you are pondering what I'm pondering.
Pinky: Poit! I guess I am.
- When Brain tries to make mice the dominant species on earth so that they'll elect him as their leader.
Pinky: Egad! Brilliant Brain! Oh wait-no, no. Why would they pick you?
Brain: Because I'm very likeable.
- From, "The Visit":
Brain: Pinky, my parents are driving me crazy.
Pinky: Really? Well, that doesn't leave with me much to do.
- The writers may have called "Yes, Always" a very expensive inside joke, but it's still a hilarious one for the viewers that do get it. The best part, though, isn't spoofing Orson Welles' commercial taping:
Producer: Brain, you're here, great! And only 45 minutes late today!
Brain: You, leave!
Producer: B-but Brain, I'm the producer!
Brain: ...Then leave very quickly.
- This Radar moment:
Brain: Now come, we are off to a place where women are paid huge amounts of money to look beautiful and change their clothes frequently.
- Not from the show, but related: Rob Paulsen let Pinky take over most of an episode of his podcast. Pinky even sang a few songs: Led Zeppelin's "Going to California", "The Star-Spangled Banner" (with all the lyrics replaced by the word "narf"), and parts of Frank Sinatra's "High Hopes" and Talking Heads' "Psycho Killer" and "Life During Wartime". All of which were awesome.
- The Brain versus Christopher Walken
- Pinky's mother and father (both voiced by Eric Idle) have some hilarious moments, but he highlight is when they're packing to go to New York, and Pinky's mother is stuffing her suitcase full of food pellets.
Pinky's Mother: I'm almost packed! We'll have food pellets for lunch, food pellets for supper, then for breakfast I thought... food pellets!
Brain: I'm told they do have food in New York.
Pinky's Father: Where is this New York? Is it far outside the cage?
Brain: It's very far away.
Pinky's Mother: Ooh, I'd better take some food pellets then!
Pinky's Father: As far as that sink?
Brain: Much, much father. We have to take a plane.
Pinky's Mother: A plain what?
Brain: An airplane! It's, uh, a giant metal bird.
Pinky's Father: Bird?! Where?! Run!
Pinky's Mother: Ah! Giant bird!!
Brain: It's not a real bird!
Pinky's Father: A bird'll swoop down and eat you right up, he will!
Pinky's Mother: Unless it's a llama!
Pinky's Father: I know — we'll feed it food pellets!
Pinky's Mother: Food pellets? Where will we get food pellets?
Pinky's Father: ...I don't know.
- Well I think so Brain, but what if The Hippo doesn't want to wear a Thong?
- The Bizarro Episode "Animaniacs Stew" pairs the Brain with Mindy instead of Pinky. He gets chased by a riding lawnmower, and tries to escape it by climbing the side of a tree. The mower simply runs right up the side of the tree after him. Seeing this, Brain looks at the camera, and deadpans "This is most unexpected."
- The intro song's variation for this episode implies Brain to be the "insane" one. "One's a small girl. The other's (beat) The Brain."
- Pinky channeling every Kaiju fan in the world in "Tokyo Grows":
Pinky: (in a stilted, Hong Kong Dub-like manner)
Narf Brain! Let us go and see Gollyzilla! We can watch excitedly as he crushes buildings and then flee in terror!
- The JSDF blasting Brain in the face when he arrives to stop the rampage of "Pinkzilla" (Pinky after Brain turns him into a kaiju).
- The Running Gag where the Raymond Burr lookalike keeps commenting on everything by saying "Yes, I see."
- This gem from the first episode "Das Mouse," where the two are piloting a submarine.
- In "Pinky's Plan," Pinky, to stop Brain from seeing the plans for his surprise party, whacks him on the head with the chalkboard.
Pinky: Sorry, Brain, there was a, um, fly on your head.
Brain: (hits him with the chalkboard) Sorry, Pinky, there was a, um, stupid look on your face.
- Al Gore the Donkey's political ramblings in 'The Megalomanical Adventures of Brainie the Pooh'.
Al Gore (in donkey costume): The president and I are pleased with the manner in which we are moving towards the 21st century, by building a very long bridge made of wood. A wooden bridge may work... This wooden bridge will lead us to prosperity... As we cross this bridge we have built, to stand upon a platform built by contribution... Though in building this bridge to the 21st century, the president and I urge caution to guard against unfounded worries about inflation...
- "You Said a Mouseful"
- Brain's plan fails because he suffers a concussion that causes him to involuntarily speak in spoonerisms. His incoherent mangling of his attempted warnings and pleas toward Pinky are just hilarious to listen to.
- The chorus who sing the usual "They're Pinky, They're Pinky and the Brain, Brain, Brain, Brain, Brain" line at the end of every episode end up Corpsing and debating on whether they should sing the title with a spoonerism to match Brain's temporary speech impediment.
- The In-Universe case in "Brain's Song"; "The O-O-O-O-O-O-O-O-OVERLORD!"