- The fact that the somewhat violent and rather scary Potty Prof got easily taken over by the calm, peaceful Placid Prof thanks to Justin's Brainwashing For Dummies book.
- Any time that Lucy and Alfendi's relationship is questioned by someone.
- Even Potty Prof, despite usually causing the tone of the game to turn serious, gets his moment in the sun during Case 002, when he starts absolutely roaring with rage over plain pasta, of all things. Mind you, there's more to it in context, but it's pretty funny nonetheless.
Funny / Layton Brothers: Mystery Room