The ending credits song, "The Massacre" by Fantomen K.
Ensemble Darkhorse: Papa Burch is one, he constantly puts down his children, kills a (zombified) man for disturbing him while reading, and alters his memory to have an excuse to punch Ash. And this is just a small fraction of what makes him so awesome. They even decided to bring him to a PAX panel due to popular demand. He received a standing ovation upon his introduction.
The Team Fortress 2 episode is presented in interview format, just like the first six "Meet The Team" shorts.
In the first part of the season 3 finale, "A Death In The Family", look at what shirt Anthony is wearing. It says "Team Blavis." Then you might realize his mistake before he does.
"Funny Aneurysm" Moment: The "World of Warcraft" episode became this due to Anthony and Ashley Burch's divorce and the controversy behind it.
Harsher in Hindsight: The Persona 3 episode, which had Ash accidentally killing herself imitating the game. When you learn about David Fetzer's (Ashley Burch's boyfriend) death by accidental drug overdose (which is debatably considered a form of suicide), watching the video depresses you even more.
The episode now warns for suicidal content via annotations
Heartwarming in Hindsight: Ash holding up the copy of Harvest Moon in the BioShock episode (and the subsequent HM episode), if you've read her How Games Saved My Life Tumblr. She opened the blog to exhibit stories of the positive influence of gaming on people's lives, and one of the first posts is her own story. Apparently anxiety problems made it very difficult for her to eat, and Harvest Moon helped her relax.
In the same vein, watching the Yoshi's Cookie episode has shades of this (with a little bit of Harsher in Hindsight thrown in) when the previously-stated anxiety is taken into account. It puts things into a new perspective considering Ash is essentially acting out being force-fed for the sake of comedy.
Hilarious in Hindsight: There was an episode where Ash makes a proposal to Nintendo on making a Nintendo Land. Years later, Nintendo Land is released as a crossover game. Of course, their E3 2012 episode lampshades this.
The Team Fortress 2 episode — Ashly eventually ended up in the series herself, both as co-writer for the Expiration Date short, and as a voice actress in said short. She plays overworked secretary Miss Pauling.
The Saints Row: The Third episode — Ashly goes into a coma from the sheer awesome of the game and recovers in time for Papa Burch to bring home Saints Row Fo', which would soon be announced and released in reality. No hitting dinosaurs with dildo lasers in the actual game, sadly.
The Adventure Games two-parter — Ashly voices Chloe Price in an adventure game Life is Strange.
Overshadowed by Controversy: Certain controversies relating to Anthony's personal life in late 2014 have become a major Never Live It Down moment for various gaming sites and forums, and is speculated to be the main reason for his departure from Gearbox Software.
Tearjerker: Ashly's story on the HAWPcast about her friend's pet wolf Wolfy going missing in Minecraft, and singing Amazing Grace with him as the sun rose in-game in front of a memorial statue for Wolfy. She goes on to say that her friend's life in-game has been consumed by his lost pet, who claims he can still hear phantom howls coming from inside the mine.
Ash's episode of the Mental Health Happy Hour podcast, discussing her boyfriend (and Must Come Down co-star's) death from accidental overdose. The worst part is when she talks about his family checking his internet history, and later informing her that his last Google search was for an engagement ring.