Spoofed in the film Kung Pow! Enter the Fist, in which the villain Betty constantly ended his sentences with "nngggggg!" and love interest Ling said "Weeee-ooo-weee-ooo-weee!". (Both were done by the voice actor to fill in the gaps made by the lip movements.) By the end of one scene, they were conversing entirely in these sounds.
In Office Space there's Bill Lumburgh, Peter's hated boss, who litters his speech with smarmy verbal tics. He begins every conversation with a shallow, "What's happening?" He always tells workers to "go ahead" and do things, to mask the fact that he's handing down disagreeable orders. After delivering an order, he tells the person, "That'd be great," as a threadbare attempt at encouragement. He also punctuates many sentences with a drawn-out "yeeeeaahhhh" or patronizing "mmmkay?"
The antagonists in Dark City, (Mr Book, Mr Hand et al) tend to close most of their affirmative or interrogative phrases with Yes?. "We remember, yes?".
Kenneth Williams in the Carry On movies and elsewhere: "Nnnnnnnnoooooooooooo, stop messin' about!"
Fingers, in the film Shira: Vampire Samurai is practically an English-speaking version of Naruto, ending almost every sentence with a hearty "Believe that!"
Mr. Deltoid, yes, from A Clockwork Orange, yes, has a tendency to insert the word yes into every sentence, sometimes at the beginning, yes, but often at the end as well, oh yes. It also begins to rub off on Alex, but more so in the book than the film.
Streets of Fire: Billy Fish says "shit" so much that it's practically a Tic.
Pirates of the Caribbean: Captain Jack Sparrow. Savvy? This expression comes from French colonists and first pirates, who added "'savez?", for "vous savez?" ("you know?", as in "get it?") at the end of sentences to make sure the natives.. well, got it.
In the sequels, Davy Jones has a tendency to punctuate sentences with an "-ah".
In The Squid and the Whale, the tennis instructor Ivan tacks "my brother" onto the end of every sentence. Eventually, he becomes a dubious role model for his young pupil Frank, who starts imitating the same goofy tic.
In Fargo, stereotypical Minnesota verbal tics are mined for comedy. Natives frequently say things like "ya," "you betcha," and "you're darn tootin'!" Natives are so fixated on saying "aw geeze" when they're upset that Wade says when he gets shot.
Number (Johnny) Five from Short Circuit has a habit of listing synonyms for various words as he speaks. Fridge Brilliance kicks in when you recall that the first serious input he got was reading an entire dictionary.
"Number 5... furious! Livid! Perturbed!"
"I have questions. Queries. Posers."
Goodfellas: Joey Two-Times. "I'm gonna go get the papers, get the papers."
In The Room, everyone greets each other by saying, "Oh, hi (name)!" often leading numerous people to say this to each other.
In the third Austin Powers movie, the eponymous Goldmember has a tendency of quoting a famous song, then whispering the copyright distractedly. "And that's the way, uh-huh uh-huh, I like it! (KC and the Sunshine Band...)"
At the end, she falls for a man named Quint who does the same thing, but the letter Q instead of P.
In Mash, Hawkeye Pierce has an odd habit of whistling during conversations. By the end of the film, Radar does the same.
Especially so with The Big Lebowski, as the BluRay comes with a bonus feature that counts every use of "Lebowski", "Dude", "Man", and "Fuck". Lebowski has the lowest count by far, though it's notably just short of 100 uses.
We're the Millers: Scotty P., y'know what I'm sayin'? It really annoys David and Rose.