Said face is pretty much modelled directly off the screaming zombie cat from Video Game Video's ident, and it was plenty creepy then. And now, just like the cat in those videos, Fluttershy's face started popping up in these.
However, videos that succeeded SHED.MOV had unfitting music as the face appeared, leading this into Nightmare Retardant to some.
The video HotDiggityDemon made for Trotcon and Everfree Northwest was supposed to be made PG-rated content. But nope! Fluttershy's face still appears at the end as a screamer. Complete with a loud scream common in a typical one.
The cartoon shown at Pon3 and Canterlot Gardens had Rainbow Dash lampshade the use of it. In which she was then interrupted by Fluttershy's face.
The end of this preview for SWAG.MOV shown at Equestria LA. Most of it is showing much of the Main cast having gone through majorCharacter Development in the time since PARTY.MOV, Twilight feeling guilty for her screw ups in MAGIC.MOV, Rarity having escaped the Mexicans a (presumably) reformed mare, Fluttershy walking out of the rehabilitation clinic, also reformed, and Pinkie Pie apparently giving up her alcohol addiction, then we get to Spike, still poking at the Rainbow Dash corpse. Turns out the whole "Twilight-plays-Frankenstein" spiel had a delayed effect, because suddenly, Rainbow Dash wakes up screaming. Which causes Spike to scream in horror. And then guess what happens.
The other way around, really. The Eldritch Abomination clearly states that no one should lay eyes on it "for sight begets curiosity, curiosity begets desire, and desire begets obsession." Jappleack is willing to die just to see what this thing is.