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Anime And Manga
- In Mnemosyne, an assassin keeps trying to kill Rin, and keeps coming back, no matter how many times she herself gets shot, stabbed, and blown up. Fortunately, Rin herself is immortal too.
- Kakuzu of Naruto isn't quite immortal, but needs to be killed five times in one battlenote before he actually dies. He's also one of the oldest living characters; he was exiled from Taki for failing to kill the First Hokage, however many years ago that was.
- His partner Hidan, on the other hand, is pretty much defined by this trope. At the climax of the series he is still alivenote , in pieces, and rotting.
- The Saint of Killers from Preacher. Not even God can kill him.
- The Kindly Ones from The Sandman, although they can only be called upon to punish a kinslayer.
- Wolverine, before he changed occupations and became a full-time X-Man.
- The Talons from Night of the Owls probably qualify, since some of them are from at least the 1800s and their healing factor seems to protect them from most methods of killing them. The Court of Owls doesn't even kill them; they instead retire them, bringing them back eventually.
- The focus of the Ranma ½ story Relentless, though the killer is a demon (or even Eldritch Abomination) known as the Reikoku, sent to perform an assassination, rather than a normal assassin who happens to be immortal. Its weaknesses: it takes a day to come back to life, and if a single person can kill it three times, it will be banished back to its home dimension. Unfortunately, each time it comes back to life, it will have developed an impenetrable defense against any attacks used against it the previous time, plus it will have developed brand new attacks, including tailor-made Flaw Exploitation attacks. It might seem like a good idea to destroy its corpse, but that just brings it back to life instantly. Oh, and if any of your friends attack it in order to help you, they get added to its hit list. And three total kills amongst you and all your friends aren't enough to finish it, it has to be three kills by the same person. Have fun!
- Inverted in What About Witch Queen? - while the assassin is absolutely mortal, his quarry proves much less so, to the surprise of them both.
- Many characters in the Highlander universe have made their living being Immortal Assassins, but most notably the Kurgan from the original film. He hires himself out to Clan Fraser in their battle against Clan Macleod, in exchange for the privilege of killing Connor Macleod himself.
- Terminator: Most terminators are specifically designed to be unkillable killers; the only way to make the T-800 in Terminator 2: Judgment Day seem vulnerable was to create an even deadlier terminator: the T-1000.
- The character "Jaws" from James Bond's The Spy Who Loved Me and Moonraker appears to be immortal. He gets a pyramid dropped on him, bites a shark (not the other way around), drives off a cliff, slams into a cable car terminal, drives a boat over a water-fall and falls out of a plane. The mooks with him drop like flies, but he just dusts himself off and goes back to work. The last we see of him, he's parachuting(?) back to Earth with his girlfriend.
- The Gholam of Robert Jordan's The Wheel of Time is basically an Anti-Magic T-1000 designed as a literal Mage Killer—so terrifying that only a handful were ever made. Only Anti-Magic artifacts like Mat's medallion (and probably starvation) can possibly do any damage, and that's not really a practical solution. It ultimately does die some time after being forced into the Void Between the Worlds that is the Skimming dimension, however, but not without a lot of ingenuity.
- Ghost, of R.A. Salvatore's The Cleric Quintet series, was a relative weakling who used a magic artifact to switch bodies, kill the target who is now in his weak body, and then heal it again using a magic ring of regeneration.
- James Spector, AKA "Demise," from the Wild Cards series. Originally killed by the Wild Card virus, he was resurrected with an experimental cure. He gained both a powerful healing factor and the ability to "project" his agonizing death onto anyone he made eye contact with, essentially killing them with a look. He was decapitated when a political assassination went wrong, and his body was destroyed by cremation when it became clear that he was trying to GROW A NEW HEAD.
- The Night Angel from The Night Angel Trilogy by Brent Weeks. He/she/it is literally immortal assassin although the immortality comes at a terrible prize: For every time he dies, a person of importance to him dies in his stead. Cue a whole lot of immortal angst and be done with it.
- The Mercenary from The Bartimaeus Trilogy, while not strictly immortal, is incredibly resilient. Through his huge Healing Factor and resilience to magic, he survived a long fall from a cliff, survived being smashed under a huge rock and survived magic that could destroy demons, let alone humans. Unlike most examples, he is not the main villain. Although even he can die to a lot of very powerful, concentrated magic.
- Messers. Croup & Vandemar from Neil Gaiman's Neverwhere are a pair of Humanoid Abominations who stalk after Door, one of the main protagonists of the book, under orders of an unknown employer. They react to injuries with amusement or annoyance, they teleport seemingly at whim, and may even possess limited time travel abilities, and they eat human flesh. Their true nature is never explained.
Mr. Croup: If we are injured, do we not bleed?Mr. Vandemar: ...No.
Live Action TV
- The television show Lexx has an immortal assassin, Kai, as one of its main characters. He literally is unkillable and one of the subplots is him finding a way to die.
- Warehouse 13 features Marcus Diamond, The Dragon to Season 3's Big Bad. Technically he can't be killed because he was already mortally wounded a long time ago, and he's using an artifact that rewound time for his body to just before his death. He's literally living on borrowed time.
- The Highlander series featured several. Christoph Kuyler claimed to be the most prolific assassin in history, as well as the less theatrical Anthony Galen and Kamir, the last of the thuggee assassins.
- The Caretaker from Alphas is virtually unkillable due to his high bone density, which he's able to regenerate so long as he has access to calcium. The downside of this is that this makes him very heavy, which Kat uses against him to drown him.
- Deadlands give us Jasper Stone, an evil Harrowed who has progressed into a full-fledged Abomination, who works for the Reckoners themselves and shoots down dangerous heroes at their bequest. He was statless for a long time, for the explicit purpose of not being killed by the party.
- Liegenstrasse in Warhammer 40K is a result of splicing human and alien genome and the prime case of Gone Horribly Right - with Casting a Shadow, Healing Factor and full Anatomy Arsenal, she proved nigh-impossible to take down and it was only managed by entire assassin temple. Maybe...
- From the point of view of the enemies, ANY player character is one of these. Even if they manage to kill you, you just resurrect at the nearest save point and keep on coming. A few examples below are justified since their means of resurrection are actually addressed within the narrative of the game:
- Raziel from the Legacy of Kain series. Killed and resurrected twice already before the events of Soul Reaver, by the time the player meets him he is a soul-devouring revenant animated by an Eldritch Abomination for the sole purpose of bringing down Kain and his empire. His physical body is essentially a half-rotted corpse, without skin, internal organs, or even a lower jaw. And if this body takes too much damage, he simply shifts to the spectral plane until he builds up enough life energy to return. And since time stops on the material plain when this happens, from anyone else's perspective he just pops out of existence and then immediately back in again at full strength. The Elder God specifically says he is "beyond death." He can't be killed because there is simply nothing left of him that's killable.
- Jack and Subject Delta from the BioShock series: functionally immortal because the game's equivalent of save points - the Vita-Chambers - are coded to their specific DNA. No one else in Rapture can use them, except Jack's father, of course.
- The player character from The Elder Scrolls V: Skyrim can seem like this, having two methods (a perk and a mask) to automatically heal from near death.
- The eponymous bad guy from The Horribly Slow Murderer with the Extremely Inefficient Weapon.