Dick:[explaining Earth weddings] The ceremony begins with the bride being given away. Sally: Excuse me? Given away? Like an object? As in, "free girl with every large fries"? Tommy: Hey, there are free girls? Can I go? Harry: Fries? Can I go?
In Western weddings, the bride's father walks her down the aisle to the altar where the vows are exchanged, a holdover from the days of Arranged Marriage. Nowadays, it's a symbol of endearment towards the most important man in her life before the groom came into the picture, and he "gives her away" to the groom. If her father is dead, unavailable, or didn't fill that role in her life, then her brother, uncle, close friend, or mentor figure are popular choices to stand in his place. It could also be her son if the bride is older.
Because this is a ubiquitous part of Western weddings, only list examples where something notable happens between the bride and her father before or during the walk, or if someone other than her father does the honor, or even examples where the groom is given away!
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When Reed and Sue Richards got married, her brother Johnny walked her down the aisle, since their parents are dead. This happens in The Movie as well, following a truly touching moment between the siblings.
When Donna Troy gets married, she has her closest friend Dick Grayson walk her down the aisle, as she doesn't have a father.
In Superman: The Wedding Album, this is one of a number of traditions Lois has a problem with, and her insistence that no-one is "giving" her to anyone is one of a number of reasons Sam Lane nearly doesn't attend. She and Clark walk down the aisle together.
One Garfield comic features Jon surprised (and angry) to see a mouse wedding occurring in his living room, but he officially draws the line when he sees that Garfield is the one giving away the bride.
In the Empath: The Luckiest Smurf story "Empath's Wedding", Empath's half-brother Brainy plays the role of giving away Smurfette as the bride.
Guys and Dolls. At the end of the movie Miss Adelaide marries Nathan Detroit and Sarah Brown marries Sky Masterson. Miss Adelaide is given away by Lieutenant Brannigan and Sarah is given away by Arvide Abernathy, an older man who works with her at the Save A Soul mission. Watch it here starting at 2:05.
The Bride in Kill Bill asks her boss/mentor/ex-lover by whom she's pregnant Bill to pose as her father for her wedding.
A similar custom exists in Inheritance Cycle, except the bride's mother takes part, as does the groom's father; each parent lists what their child is bringing to the marriage (the groom's land, the wife's dowry, etc.) When Roran marries Katrina they both have stand-ins, however, since their respective parents are deceased. (Roran has Horst, an older male friend and father-figure, and Katrina has Birgit, who was a friend of her mother's.)
Around the World in Eighty Days ends with Phileas Fogg and Aouda marrying, with Passepartout giving the bride away, since he was the one who saved her from being ceremonially burned alive.
In Catching Fire the Capitol asks Haymitch to give Katniss away at her wedding to Peeta (her father is dead) and he readily agrees. We don't find out if he does the honors at their actual wedding after the main story ends.
Live Action TV
Phoebe from Friends asked Joey to give her away, because her stepdad was in prison. Joey, however, ends up officiating the ceremony (he's an ordained minister through the Internet) so Chandler does it.
In an earlier episode of Friends, Ross gives his ex-wife Carol away when she marries Susan, because her parents don't approve of the lesbian wedding.
NCIS: When Ziva is considering marrying Ray, everyone assumes Gibbs would walk her down the aisle. It does not happen, and never will. Marrying Ray, that is, not Gibbs walking her down the aisle. Still holding out hope for the second bit.
In Mash Col. Potter gave away the bride in an otherwise completely traditional Korean wedding. (Dunno if that's a custom in Korean weddings or not.) He had vetoed the wedding taking place in the compound; BJ said "but she wanted you to give her away," at which point he relented.
Col. Potter also gave Margaret away when she married Donald Penobscott.
In the Buffy the Vampire Slayer episode "Something Blue," Buffy and Spike are planning their wedding (It Makes Sense in Context), and Buffy asks Giles to give her away. Unlike them he realizes this entire discussion is ridiculous and that they must be under some sort of spell...but he's still touched.
Lwaxana Troi on Star Trek: The Next Generation wanted to hold her wedding aboard the Enterprise and asked her other crush, Captain Picard to give her away. Picard granted permission because, "Nothing would please me more than to give away Lwaxana Troi."
In the episode where she leaves The Drew Carey Show, Kate is getting married to a man but her parents don't attend because they think it's too rushed. Drew, despite having been married to her and still loving her, gives her away at the ceremony and wishes her a happy life.
On The West Wing President Bartlet almost did not get to give away the bride at his own daughter's wedding due to an international crisis he had to take care of. In the end he had to plead with two world leaders to hold off war just long enough so he could walk his little girl down the aisle.
Though the wedding between Angela Martine and Robert Tomlinson in the Star Trek: The Original Series episode "Balance of Terror" is interrupted by a red alert, we do get to see Scotty give Angela away.
In The Sarah Jane Adventures episode "The Wedding of Sarah Jane Smith" (Whoniverse titles sure are repetitive, aren't they?) Sarah Jane asks her son Luke to give her away. He asks why she doesn't choose the Doctor instead, and she says she wants someone reliable.
Sarah Jane: Besides, where would I send the wedding invitation? Metebelis 3?
In the Highlander episode, "Til Death". It involved two Immortals who had been married for nearly 300 years, Robert and Gina de Valincourt. In the flashback when the two announce their intention to marry, Gina asks Duncan and Fitz to give her away. This shocks both of them, as the two had been competing for her favor earlier in the episode and both expected they were going to be the one Gina would marry.
In the country song "You Can Let Go Now Daddy" the second verse is about the narrator being escorted down the isle. When they get to the altar, her father wouldn't let go of her hand until she tells him the title of the song.
The last verse of "Butterfly Kisses" has the father (and narrator) giving away his daughter at her wedding.
Mamma Mia!: partly because the main character doesn't know who her real dad is, partly because he wasn't there when she was growing up, regardless, she asks her mother to give her away.
In the ending of King's Quest VI, if King Caliphim and Queen Allaria are brought Back from the Dead, they give their daughter Cassima away to be wed to Prince Alexander. If Cassima's parents aren't rescued, Jollo acts on behalf of King Caliphim.
It's also notable in that the Green Isles also asks someone to speak for the groom as well. If Alexander rescues the genie, then Graham does the honors. If not, then Captain Saladin of the guard dogs will speak on Alex's behalf.
An aversion in the Fan SequelThe Silver Lining. Rosella refuses to be given away, choosing to walk down the aisle by herself. Unfortunately, this leaves her family too far away to stop the mysterious stranger's attack...if they could have prevented it at all.
In Something Positive, Aubrey asks Mr. MacIntire, her best friend's father, to give her away at her wedding. Her own father is never seen or really mentioned, so it's unclear why he didn't do.
In Family Guy, Jillian, whose parents are dead, asks Peter to give her away. Justified in that they have a rapport due to their common stupidity.