- When Nedry and Dodgson meet before Nedry leaves for the park:
Dodgson: You shouldn't use my name.
Nedry: [waving his hands and pointing]
Dodgson! Dodgson! We've got Dodgson here! [beat]
See? Nobody cares.
- In the iconic scene where everyone sees their first live dinosaur, Ellie is very preoccupied with the leaf of an extinct plant she picked up. Finally, Grant grabs her head and physically makes her look. Cue dropped jaw...
- Even better, Grant dumbfoundedly pointing at the brachiosaur and numbly mumbling:
Alan: It — it's a dinosaur.
- From early on:
Ellie: What are you thinking?
Alan: That we're out of a job.
- Reality Subtext: That was inspired by a Real Life statement by head of the original stop-motion animation team hired for the movie when they saw the initial CGI work.
- The robot arm in the dinosaur nursery taking the hatched egg right out of Grant's hand and setting it back down. The look on his face...
- This gem from early on, after Malcolm, Sattler, and Grant all argue against Jurassic Park:
Hammond: I don't believe it! Hah! I don't believe it! You're supposed to come here and defend me against these characters and the only one I've got on my side is the bloodsucking lawyer!
Gennaro: Thank you.
- Later, when the dinosaur tour falls flat thanks to the non-appearance of the dinosaurs:
Malcolm: Ah, now eventually you do plan to have dinosaurs on your, on your dinosaur tour, right? Hello? [taps the camera lens, breathes on it] Hello? Yes?
Hammond: [deadpan] I really hate that man.
- This famous exchange, but especially right after with Ian and Alan turning to stare at her, and Alan's little smile:
Ian: God creates dinosaurs. God destroys dinosaurs. God creates man. Man destroys god. Man creates dinosaurs.
Ellie: Dinosaurs ... eat man. Woman inherits the earth.
- Ian's utterly deadpan reaction to the Triceratops feces:
Ian: That is one big pile of shit.
- Gennaro bolts upon seeing the T. rex and hides in a toilet cubicle. Alan and Ian see this, but not the T. rex.
Alan: Where does he think he's going?
Ian: When you gotta go, you gotta go.
- After Grant retrieves Tim from a car stuck in a tree, the branches snap and the car comes loose, crashing down through the tree and landing on top of them (luckily the roof was missing, creating a safe spot), and then...
Tim: Well ... we're back ... in the car again.
Grant: At least you're out of the tree.
- The moment when Lex decides she wants to stroke the Brachiosaurus after seeing Grant and Tim do it... and she promptly sneezes all over her.
Tim: God bless you!
- Hammond's reaction when the park's fences begin shutting off:
Check the vending machines.
- When attempting to fix Jurassic Park's computer problem:
Nedry: [on computer] Unh-unh-uh, you didn't say the magic word! Unh-unh-uh. Unh-unh-uh...
Arnold: PLEASE! Goddamnit, I hate this hacker crap!
- Ellie and Muldoon discovering the injured Malcolm:
- This exchange:
Hammond: When they opened Disneyland in 1956, nothing worked right.
- When Dennis Nedry slips down the slope after his jeep gets stuck in the rain, there is a cartoonish whoosh clearly audible.
- It's unknown if this was intentional, but towards the end, one of the jeeps has mud covering parts of the Jurassic Park logo, so it looks like it says, "UR ASS PARK".
- Alan scaring the kids ... by pretending the 10,000 volt electric fence is switched on when he touches it.
- Nedry attempting to distract the Dilophosaurus ... by trying to get her to chase a stick.
Nedry: [as Dilophosaurus stares at him] Ah, no wonder you're extinct! I'm gonna run you over when I come back down!
- At the end as they flee the visitor center:
Grant: Mr. Hammond, after careful consideration I've decided not to endorse your park.
Hammond: So have I.
- Right before that, as the "When Dinosaurs Ruled the Earth" banner flutters down past the Tyrannosaurus.
- When the Tyrannosaurus is chasing a Jeep and she appears in the "Objects in mirror are closer than they appear" mirror. As if she could be any closer already.
- Muldoon's smug expression when the T. rex gives up the chase and Malcolm's "Think they'll have that on the tour?" add a bit of icing to the cake.
- After learning that all the dinosaurs are female.
Ian: So how do we know? Does someone go out into the park and pull up the dinosaur's skirts?
- When the the dinosaurs start rampaging, the protagonists are unable call for help or evacuate because all the phones are down, courtesy of Nedry's virus. When they finally reestablish communication, Grant informs Hammond via phone ... who fails to comprehend that they can finally leave the island.
- Really, we could just say "Every other line Malcolm says in this movie."
- When trying to tell Ellie where to go to restart the park, Hammond pulls out a map and places it over Malcolm's already broken leg, who then gasps in pain. Hammond gives him an apology that doesn't sound quite sincere.
- The blasť way that Ian asks Grant and Ellie about their job, whilst eating peanuts on the helicopter. Made about a hundred times more hilarious by Jeff Goldblum's weirdly fascinating laugh.
- Darkly humorous moment from the first Tyrannosaurus rex scene:
- When the T. rex breaks the fence, Malcolm laments he has been vindicated.
Ian: Boy, do I hate being right all the time.
- During the tour, Gennaro gets a little confused about the lab scientists:
Gennaro: Are these characters ... auto-erotica?