Ogura Sio/Oda Nobunaga ("Nobunagun")
Sio voiced by: Shiori Muto (Japanese), Jad Saxton (English)
- Action Girl
- Ambiguously Bi: Has definite Ship Tease with both Adam and Asao.
- BFG: Her AU weapon.
- Boyish Short Hair
- Brainy Brunette: Zig-zagged, for she is prone to falling asleep in class but is a very capable strategist.
- Cute and Psycho: Sports a killer Slasher Smile when she goes into battle.
- Friendless Background: She spent much of her time in school alone, reading magazines on military weapons, and people thought her weird as a result and didn't really hang out with her.
- Hypocritical Humor: She believes she is that the only normal E-Gene holder...
- The Gunslinger
- More Dakka: One gun isn't enough? Another! Still not enough? Another!
- Not a Morning Person: Frequently wakes up late.
- Otaku: For weapons, and military items in general.
- Small Girl, Big Gun: The cartridges alone are bigger than her torso.
- Smitten Teenage Girl: Towards Adam.
- Socially-Awkward Hero: She's not very good interacting with people, especially those as strange as her fellow E-Gene Holders.
- The Strategist
Adam Muirhead/Jack the Ripper
- Blade Below the Shoulder
- Eleventh Hour Superpower: In the final episode he reveals abilities related to the true identity of Jack the Ripper.
- Green Eyes
- Jerk with a Heart of Gold
- Mystical White Hair: Being an E-Gene holder.
- Slasher Smile: Very prone to these during battle.
- Swiss Army Weapon: His blade can split apart into a claw of sorts, made of different kinds of blades.
- The Tease: Not as much as Gandhi, but it shows.
- Wolverine Claws
Mahesh Mirza/Mahatma Gandhi
Voiced by: Nobunaga Shimazaki (Japanese), Christopher Bevins (English)
- Barrier Warrior: Although he thinks calling it a barrier is lame.
- Cool Headgear: His AU weapon.
- Insistent Terminology: "It's not a barrier!"
- Mr. Exposition: He explains the bases and the platoons to Sio.
- The Tease: He tends to jokingly flirt with Sio.
Jess Beckham/Isaac Newton
- Action Girl
- Ambiguously Bi: She apparently has no problem kissing people of either gender.
- Braids of Action
- Forceful Kiss: Her way of greeting people is with a French kiss.
- Giant Foot of Stomping: Her weapon seems to be one crossed with an abacus. For extra stomping power, it can apply Selective Gravity to whatever it steps on.
- Gravity Master: Her AU weapon's power.
- Cool Shades
- Kick Chick: Due to the nature of her AU weapon.
- The Paralyzer
- Break the Haughty: Is not at all pleased when Sio shows him up on a way to beat the E.I.Os.
- The Chessmaster
- Great Detective
- Jerkass: Definitely has moments of this though its mainly because of a The Needs of the Many mindset.
- The Leader: Acts like this on the missions.
- Mission Control
- The Needs of the Many: Has this mindset.
- Nerves of Steel
- Non-Action Guy
- The Smart Guy
- Doing Research
- Pet the Dog: To Galiko.
- What the Hell, Hero?: To Vidocq due to his disregard of Galiko's safety during a mission.
- Fish out of Water: As a Non-Action Guy, he feels like this during the "Stone Forest Operation", which result in Jack making fun of him.
- Bratty Half-Pint: She can be rude and haughty towards Sio, since she was the previous newbie, despite the fact she appears to only be in elementary school.
- Cat Smile
- Clingy Jealous Girl: Isn't too fond of Vidocq interacting with Sio.
- Eye Scream: Part of her AU weapon is smashed up by an Invasion Object, leaving her severely injured.
- Girlish Pigtails
- Mission Control: On missions, because her AU weapon is not combat-based.
- Precocious Crush: On Vidocq.
- Token Mini-Moe: Although she insists she's Older Than She Looks.
- A Day in the Limelight: Her debut episode
- Alpha Bitch: Though it's mostly toward Gaudi.
- An Axe to Grind
- Blade Below the Shoulder: In the form of a tomahawk.
- Dual Wielding
- Only One Name: Is referred to as Lemond by Hunter in episode 13. It's unknown if this is her first or last name.
- Slasher Smile: Like Nobunagun and Jack, she too tends to smile sadistically while in combat.
- Smug Super
Ibukigin "Esui" Erdenbileg/Cyx
- A Day In The Lime Light: Her debut episode
- Beta Bitch
- Biker Babe
- Cool Bike: Her AU weapon.
- My Horse Is a Motorbike: Cyx is based on the Mongolian story "Suho's White Horse" which is why she rides that white vehicle and they call her Suho.
Voiced by: Ayumu Murase (Japanese), Sean Michael Teague (English)
- A Day in the Limelight: His debut episode.
- Anime Hair: Has green spiky hair.
- Butt Monkey
- Dishing Out Dirt
- Imagination-Based Superpower
- Loving a Shadow: Develops a crush on Sio before even meeting her and is disappointed by the reality of her. (Though this is mainly because he had a shopped photo of her and was not happy when he found out she wasn't as endowed as the photo implied.)
- Reality Warper
- Tag Along Kid
- Cynical Mentor: He's a bit of a Jerkass when Sio meets him, and to the end, he expresses doubt over whether or not Sio is worth the fuss. However, he recognizes her great potential and seems motivated to train her so she doesn't get butchered in real combat.
- Dirty Old Man: Actually, this is invoked. He really has no (specified) interest in Sio, but he uses lecherous and perverted tactics to motivate her into completing her training when she starts slacking off.
- Master of Illusion: He has the ability to produce illusory copies of anything he captures within his camera.
- The Mentor: He's put in charge of training Sio immediately after she joins DOGOO.
- The Atoner: After seeing her hometown destroyed twice in the space of 1500 years, she becomes determined not to let it happen again, and to protect Earth from the Invasion Objects.
- I Was Quite a Looker: Flashbacks to her youth reveal she was very beautiful.
- Really 1700 Years Old: Thanks to several periods of stasis.
- Badass Normal
- Complete Immortality
- Improbable Aiming Skills
- Older Than He Looks: By about 1000 years, according to Episode 10.
- Stern Teacher
- The Stoic: He barely reacts to anything.
- Stoic Spectacles: He's never seen without his noteworthy glasses.
- Tall, Dark and Handsome
- The Comically Serious
- Undying Loyalty
Evolutionary Invasion Objects (E.I.O)
- Alien Invasion
- Attack of the 50-Foot Whatever: The motherships in particular tend to be very huge in size.
- Combat Tentacles
- Evil Evolves: And they're close to getting on land.
- Hive Mind: Actually, it's thanks to some messenger Objects that spread news of defeat, but it acts like this in spirit.
- It Can Think
- Mook Maker: Several are able to make eggs that produce even more.