- Defictionalization: A page on the game's official website discusses how MEWTRAL, a two-girl Virtual Youtuber group who debut in this game, are planned to become actual Virtual Idols with musical careers.
- No Dub for You: Neptunia Virtual Stars is first title in the series to not be dubbed into other languages. This is likely due to the fact that actual Vtubers are part of the cast, though the COVID-19 Pandemic making English dubbing harder may have also been a factor.
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