- Young Blu and all of the other birds getting captured by poachers.
- Blu after he and Jewel are freed from the chain, with him stuck on the ground while his bird friends fly.
- A heartbroken Jewel crying as she flies off after her de-chaining and argument with Blu.
- What makes this even more heart-wrenching is that if you remember how Jewel first met Blu, she couldn't stand him. And now she's crying because she thinks she'll never see him again.
- Linda failing to stop the smuggler's plane—with Blu inside—from taking off and watching hopelessly as it flies into the distance and she sobs on Tulio's shoulder.
- Rafael after getting captured by Nigel along with his friends, solemnly apologizes to Eva that he "won't make it back in time for dinner".
- Linda releasing Blu. It's mostly intended to be a heartwarmer, but for just a moment, she looks like she's about to cry.Linda: That's my big, brave boy.
Tearjerker / Rio