- The Toiletnator being the Warm-Up Boss. Given the fact that he's a Harmless Villain who could never catch a break in the game's source material, it's hilariously fitting that he serves as the boss of the tutorial level.
- Knightbrace's frustration at Numbuh Three learning how to stop his Bug-Brite toothpaste scheme from the fireflies.Knightbrace: How could such tiny bugs have such big mouths?
- Numbuh Four's incredulous ire at the Toiletnator's poorly-conceived revenge scheme, especially when he calls him out on the ridiculousness of having sentient toilet paper as his henchmen.
- Father's reaction to seeing the Toiletnator among the captured villains.Father: What's the Toiletnator doing here? He'll ruin everything!
Funny / Operation: V.I.D.E.O.G.A.M.E.