My argument is that we don't split tropes by how they are played with, in that sense it doesn't matter if it is parodied or played for laughs or played with a ham sandwich there needs to be a much harder line. The Guy Flick idea was about dividing it between "meant to appeal to guys because of it's masculinity" and a particular element that demonstrates extreme manliness. It's like the difference between a plot trope and a character trope. All tropes may be played with but that doesn't mean that if something is played for drama it is somehow a different concept.
Does Testosterone Poisoning have to be limited to works only? Iconic characters like Negima's Jack Rakan and Full Metal Alchemist's Major Armstrong clearly serve in part as parodies of exaggerated masculinity, and there are others like them.