These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
The fish from You Remember Me? You know, a lot of characters on this show are played up to this.
The mad doctor himself. From bringing a chicken back to life solely to watch a bunch of bad television then kidnap his wife to endure the same torture he escaped from.
The little girl in Sunny Muffins
May I propose making their own monster page, since there would be too many to list here?
A huge example came with a sketch making fun of the Challenger accident. The episode aired days after the 25th anniversary of said accident. Fans were not amused.
Many skits can become this for people by having the situation become disturbingly violent, particularly in the more recent seasons, with the Joker Execution in Season 4's Catch Me If You Kangaroo Jack being a more hated example for this.
The Toy Story 4 sketch. It involves Andy and his girlfriend having sex in his bedroom, much to the toys' horror. The worst part, however, is when Andy uses Buzz as a bong, irreversibly damaging the latter's brain. And Woody's the one putting Buzz out of his misery.
The NORAD vs. Santa sketch. Especially the ending.
The Zombie Idol Sketch. Especially the part where zombie Kurt Cobainblows his head off with a shotgun. Even the writers on the DVD commentary admitted they had reservations about that joke.
Hilarious in Hindsight: A season 4 sketch featured Dakota Fanning challenging Triple H to a wrestling match in John Cena's place, claiming she was only at the event to promote her new movie. The sketch debuted on a episode of Monday Night Raw, where Seth Green had placed himself into a tag team match with Cena & Triple as his partners, when he was only at the show to promote Robot Chicken as one of Raw's first Guest Hosts (Celebrities appearing on the show in order to promote their latest works, or because they were Local Sporting Heroes).
There's a segment with Lil Hitler where he tells his teacher that he needs a Polish boy's desk. Cut to a Polish boy next to him, sweating. You'd think sweating wouldn't be funny, but when someone's sweating clay (or whatever that is), it really IS unsettling. This is also Nausea Fuel.
The Gummy Bear has...a rather horrifyingly painful scream for some.
Darkwing Duck being cooked alive in the oven with Gosalyn, his daughter, watching in horror. Then at the end, he is served to her and Michael Moore and is decapitated and Gosalyn's scream echoes into the credits!
Weird Al Effect: The series has become sort of a Weird Al Effect making machine almost on the level of Alice in Wonderland. It doesn't help that a fair portion of it's audience has never seen the 80's cartoons it parodies.