- Alternate Character Interpretation: Quite a lot, one of the most obvious being the proposition that the two unicorns are in fact a pair of sadistic psychopaths bent on psychologically torturing Charlie through their facade of being sickening cutesies. See the Wild Mass Guessing page for more.
- More or less confirmed by the fourth episode.
- Awesome Ego: The millipede is vain, but she's quite a great singer.
- Big-Lipped Alligator Moment: Too many to count.
- Crosses the Line Twice: In episode 4, Blue straight-up electrocuting Charlie while Pink shouts excuses is more... overt than before.
He's resisting arrest!
He's got a gun!
He's got another gun!
- Crowning Moment of Awesome: Starfish's Heroic Sacrifice in episode 4. Not only does he save Charlie from the duo's most twisted stunt yet, but he's the only person in the entire series who has ever managed to get the better of them in any way.
- Crowning Music of Awesome: The Millipede Song.
- Drinking Game: Try watching all four of them in a row, taking a drink every time Charlie is named.
- Ear Worm: All songs are, but "In The Ocean Blue" deserves a honorable mention.
- And now we have the Millipede song.
- Genius Bonus: The sea goat thing from "Charlie the Unicorn 3" is a Capricorn, a mythological creature from Greek mythology.
- Hilarious in Hindsight: The Millipede is not the only giant sparkly narcissistic arthropod to sing about how amazing and fabulous they are.
- Jerkass Woobie: Charlie is rather surly and snarky, but it's difficult to not feel sorry for him with the crap he has to put up with.
- He's demoted to just plain woobie in Charlie Teh Unicron, where he's less surly and snarky and more submissive and quiet.
- Memetic Mutation:
- "Shun the non-believer!"
- Pretty much everything in the series, really.
- Moral Event Horizon: The blue and pink unicorns cross it in Charlie The Unicorn 4 when they leave Charlie to be blown up on the moon, after they promised that they would never bother him again
- Nightmare Fuel: The strange green fuzzy thing that guards the temple of the Banana King in the second short. Its bloody, lipless mouth and lidless eyes call to mind some sort of dreadful Body Horror. It's even more unsettling that its entrance triggers a very long period of silence, during which Charlie starts to panic as he wonders whether he's the only one seeing this.
- Squick: The pink and blue unicorns ask Charlie to grab on to their absurdly long tongues twice in the third video. This is Charlie's reaction.
- Too Dumb to Live: Charlie does keep hanging out with the unicorns despite the fact that they keep injuring him several times.
- On the other hand, the pair's reality warping powers give the impression that, regardless of what Charlie decides to do, they're going to force him to come along anyway. Charlie himself states that he has no choice, so maybe he thinks submitting might be less painful than resisting.
- Uncanny Valley: There's something downright unsettling about that millipede's face.
- Ugly Cute: The sea... goat... thing. Which looks a lot like Capricorn.
Charlie: I can't tell if you're adorable or creepy... probably gonna go with creepy.
- Viewer Gender Confusion: The unicorns are often mistaken for a Pink Girl, Blue Boy when they're really ambiguous in canon.
- The Millipede has a rather androgynous face, and calls herself a king in her Bragging Theme Tune, but also mentions laying eggs and the backup singers use female pronouns.