Helvetica Black from the Robert Rodriguez kids' film Shorts. Let's see: female bully whose main target is her love interest? Check. "I'm not helping you because I like you or anything"? Check. Freakin' twintails? Check, check, check.
Margaret Tate in The Proposal. Known as the "Witch" around her publishing house for being a crazy, defensive, angry bitch, but later on switches to total deredere in regards to her male secretary.
Reepicheep in the film version of The Voyage of the Dawn Treader has definite tendencies here, as most of the time he is an overexcited and combative sort but shows a much nicer side when Eustace has an attack of depression after being turned into a dragon.
The Spirit of Christmas Present as played in Scrooged by Carol Kane.
Prudence Perkins and Patience "Sidewinder" Barton in Support Your Local Sheriff and Support Your Local Gunfighter respectively. Both are hot-tempered women who almost instantly latch onto James Garner's two characters as potential mates. One notable difference between them is that Prudence is a much better shot.
A bit of Foe Yay is induced in the film version of The Hitchhiker's Guide To The Galaxy, since one of Zaphod's heads seems to generally like Aurthur (even complementing him and not saying much bad about him), while his second head threatens to pull out his spleen and rips on him constantly. This is somewhat lost in the book since both heads think the same way and both generally hate him.
Or rather, it was never there to be lost in the first place, since the book came first.
Claude Frollo from most versions of The Hunchback of Notre Dame. Especially apparent in the Disney one, where one of his songs, 'Hellfire', switches rapidly from "I want Esmerelda to die" to "I just plain want Esmerelda".
Neytiri from James Cameron's Avatar acts quite tsuntsun towards protagonist Jake Sully. She even refers to him as "skxawng," which is essentially Na'vi for Baka. She softens up to him once they get to know each other better, and eventually she gets to show her deredere side. (Played with here.◊)
Klara Novack from The Shop Around the Corner is like this to Albert Kralik. While she's extremely acerbic in person, she shows her deredere side in their anonymous letters. Jeanette Mac Donald's character in The Merry Widow also has some tsundere tendencies, while Marion Hopkins in The Smiling Lieutenant is more of a type B. Either Ernst Lubitsch or Samson Raphaelson really loved this trope.
In a case where the tsundere is the protagonist, Ginger from Chicken Run is very much this towards Rocky. Though considering his cockiness and Fake Ultimate Hero status, he's pretty much asking for it.
Grumpy from Disney's Snow White is an early, definitive tsundere played completely straight, as well as being a rare male example.
Gru from Despicable Me, though he's not a love interest, he's a father-figure.
EVE from WALL•E is a non-human example. She helpfully illustrates why you do not give one of these ladies a plasma cannon.
The repair scene at the end (particularly where she blows out the ceiling to get him sunlight) is a perfect example of the "Ohmygodhe'sintroubleGETTHEFUCKOUTOFMYWAY" protectiveness Tsundere can exhibit when their love interest is threatened.
Most Tsundere girls at least WAIT for their love interest to say or do something stupid to break out the giant mallet from hammerspace. EVE uses it at their first meeting to say 'hello'.
Kate Mosely in The Cutting Edge is just an absolute bitch on skates at the beginning of the film, although it does turn out that she has a deeply buried sweeter, kinder side. Of course, by the end of the film, she's completely warmed up.
Celia from Monsters, Inc. is generally sweet and affectionate towards Mike, but she gets very angry after he leaves her to be picked up by the CDA decontamination team.
The Landlady from Kung Fu Hustle. Well, more like a cranky old woman very early on, getting pissed off at the residents complaining about the lack of amenities and screamingreallyloud for them to all shut up (and it's later a Plot Point), not to mention slapping the crap out of her husband whenever he hits on a bucktoothed resident lady of the complex. But, she's shown to be protective and not a jerk later on in the movie.
Though she may have had reason for suspecting him, since he has apparently spied on women in the showers, which is mentioned early on, and the character of Rabbit-Tooth Jane, as the buck-toothed girl was known (for obvious reasons) was a Lady of the Night.
This troper guesses that Leia counts, since she's listed above under both A and B.
In the 1996 film version of Jane Austen's Emma, the title character finally realizes she is in love with her lifelong friend Mr. Knightley, but wavers back and forth, repeatedly saying "I hate John!", then "I love John!" and takes a long time to decide whether to pursue marrying him. This troper is unsure whether she would be classified as a Type A or Type B, however; as Emma is very attached to and jealous (possessive?) of Mr. Knightley, but completely opposed to a romantic attachment between them for much of the film.
