For the Evulz: The Bots claim they're transporting Mike's most treasured possessions someplace else with their homemade projector, but they're likely just burning them all up for a laugh.
Genre Savvy: Mike manages to realize this by the end, and then tricks the Bots into destroying something Servo loved.
Fun with Acronyms: Upon seeing that the director of photography is Stanley Pavey, B.S.C.:
Crow: Stanley Pavey—Big, Stinky, Crappy Director of Photography!
Jerkass Has a Point: Blanchard cuts Paul's funding and tells him to dismantle his equipment, a serious dick move considering Paul's demonstration was sabotaged. However, Mike and the bots agree with Blanchard around the time Paul tries to recklessly perform the experiment on himself.
Later, Crow amends this — Blanchard should have held a proper hearing first. In fact, he thinks that was the whole movie's Aesop.
Lame Pun Reaction: When the police are mystified by the inexplicable electrocution deaths of three crooks, Crow is quick to quip "Shocking, isn't it?" Mike, as usual, didn't appreciate the pun.
During the credits, Mike deliberately reads the names wrong, until Crow tells him to knock it off.
"I want you."
Prof. Lembach, and whether or not he's staying much longer. There's even a host segment dedicated to this.
Paul's flaming orange hair
Paul's "panic" supposedly excusing his murder of 11 people and a cat.
Trying to teleport a girl's knickers as part of an experiment.
Several riffs based on the line, "Pretty you may be!"
Secret Legacy: While searching the castle, Pearl discovers that she and Clayton weren't the first Forresters trying to drive people mad:
Pearl:Amethyst Forachenta Forrester trapped a man in a cave and pushed in bad paintings of the hunt.
Take That: At the very beginning upon learning they're finally back in the present day, Mike and the Bots rejoice over the fact that they can watch Ethan Hawke movies again, only to immediately take it back by stating that's actually a horrible fate.
Theme Naming: Apparently all female Forresters are named for gems and precious stones.
Touch of Death: Crow develops this. Mike thinks he's having another one of his 'episodes', but is electrocuted upon touching him and dies. Luckily, Crow is able to revive him by touching him again.
Unwanted Rescue: Mike is pretty upset about being brought back from the dead, or at least being brought back to watch this movie.
Vandalism Backfire: Crow and Tom Servo claim to have invented a "projector" (i.e. a teleporter) but it actually just burns everything they're "projecting". Crow and Servo insist on demonstrating the device with some of Mike's most prized possessions. Mike eventually catches on and tests the device on one of Servo's prized possessions instead