The scene where Frozone is looking for his super suit.
"Honey! Where's my super-suit?" "What?" "Where? Is? My? Super? Suit?" "I, uh, put it away." "Where?" "Why do you need to know?" "I need it!" "Uh uh! Don't you even think of runnin' off and doing no daring-do! We've been planning this dinner for two months!" "The public is in danger!" "My evening's in danger!" "YOU TELL ME WHERE MY SUIT IS, WOMAN! WE ARE TALKING ABOUT THE GREATER GOOD!" "GREATER GOOD?!? I AM YOUR WIFE!!! I'M THE GREATEST GOOD YOU ARE EVER GONNA GET!!!!!!"
Every single thing the kid on the tricycle says. Special points to this one:
Kid:That was totally wicked!
Elastigirl after she infiltrates Syndrome's base. She cleverly dodges trains and hides from guards with ease, and as she turns around to move forward, she notices herself in a mirror... where she sighs unhappily at the sight of her butt. Looks like Bob isn't the only one who put on weight during retirement.
Which turns into a Brick Joke in a later scene between Mr. Incredible and Syndrome.
Syndrome: Now you respect me, because I'm a threat. Turns out there are a lot of people who want respect, and they'll pay through the nose to get it. How do you think I got rich? I invented weapons, and now I have a weapon only I can control and when I unleash it- [gets cut off as Mr. Incredible throws a tree trunk at him. He dodges and freezes Mr. Incredible in place.] You sly dog! You got me monologuing, I can't believe... [chuckles]
The montage of heroes who had... bad luck with capes.
Then there's Edna during the USA versoins anti-piracy notice:
Edna:If you mess with the Incredibles they will hunt you down to the ends of the earth and KILL YOU LIKE A DOG!
Helen's slack jawed horror and Edna's maniacal glee in the scene where Edna shows off the rest of the family's super suits.
After the action opening sequence with Bomb Voyage:
Police Officer: Wait, you mean you didn't catch him? Mr. Incredible: Yeah, Skippy here made sure of that. Buddy: Incrediboy! Mr. Incredible: YOU'RE NOT AFFILIATED WITH ME!
And who could forget?
Bomb Voyage: Et ton costume est complètement ridicule! (Translation: And your costume is absolutely *ridiculous*!)
"Bomb Voyage!" "Monsieur Incroyable!"
Plus this family exchange:
Violet: The only normal one is Jack-Jack, and he's not even toilet-trained! Dash: Lucky. (Helen and Violet give him an odd look) I mean, about being normal.
"Elastigirl? You married Elastigirl?" (laughs, then looks at the kids) "Oh, and got BIZZAY!"
Mr. Incredible's and Frozone's RiffTrax to their cartoon short:
Mr. Incredible: I was told it was a new process! Frozone: And what would that new process be, Mr. I? Cheap-o-rama?
And the first appearance of Frozone...
Frozone: Wait, is that me? I'm white...they made me a white guy?! Mr Incredible: You're... black-ish. Frozone: They made me a white guy! Mr Incredible: Well, maybe the print's faded. You're tanned... ish.
Frozone: Oh, oh, oh, I get caught. The black superhero gets caught. Mr Increcible: Well, a minute ago, you were complaining they made you white! Frozone: Oh, that's right... the tanned superhero gets caught!
Frozone: DID YOU OKAY THE RABBIT?!
Mr Incredible: I HAD NOTHING TO DO WITH THE RABBIT, I DON'T KNOW WHERE THEY GOT THE RABBIT!
Frozone: WELL SOMEBODY OKAYED THE RABBIT!
"The rabbit is cuddly! Kids like little, cuddly sidekicks. I mean... the rabbit... it's a time tested... okay, the rabbit bites."
After escaping the volcano, the kids spend the night out in the jungle. In the morning, Dash wakes up first...and realizes with disgust that he's cuddled up with Violet in his sleep.
Don't put your hands on the roof of your car. Especially when you have super-strength.
The guards in the mobile van that the family steals are watching a TV with the Omnidroid destroying Metroville, while sipping a bottle of champagne.
At the end of the movie, Dash is finally able to try out for track, and his family is on the bleachers cheering him on... and warning him not to run too fast. The look on the other parents' faces as they listen to the Parrs' instructions of "Make it second! A close second!" is priceless.
Nearly the entire conversation between Bob and Mr. Huph.
Bob: Did I do something illegal?
Huph: (through gritted teeth) Nnnnno...
Bob: Are you saying that we shouldn't help our customers?
Huph: The law requires that I answer 'no'...
In a display of literally superhuman self-control, it takes Huph being callously dismissive of and then openly mocking the victim of a mugging before Bob throws him through five walls and into a filing cabinet. He's several times the size of Huph, but it's still really cathartic (and hilarious). One of the commentaries talks about how the guy who animated it seemed to be working out an issue or two.
Earlier, when Bob's helping the little old lady to penetrate the bureaucracy, and she begins thanking him profusely:
Bob: Shh! [Standing up;] I'M SORRY, MA'AM! I KNOW YOU'RE UPSET! [whispered, to her] Pretend to be upset. [The woman leaves his cubicle wailing melodramatically... and walks right past Mr. Huph, who doesn't notice her.]
After Helen rescues Bob and they're running through the jungle looking for the kids while arguing.
Bob: I should have told you about being fired, I admit it, but I didn't want you to worry.
Helen: You didn't want me to worry?! And now we're running for our lives through some forsaken jungle...
Bob: (grinning at her) You keep trying to pick a fight, but I'm still just happy you're alive!
Moments before, when Helen finally catches up with Bob, only to find her embracing Mirage (out of relief that his wife and children are still alive). Upon confronting an extremely pissed-off wife, Mirage is under no illusions about the position she's found herself in:
Mirage:[Nervously] Hello! You must be Mrs. Incredible. I'm— [From off-screen, Helen punches her in the face — from the other side of the room — causing her to crumple like a sack of potatoes.]
"I am Syndrome! Your nemesis! And... (inadvertently throws Mr. Incredible out of sight) oh, brilliant."
The entirety of the Jack-Jack short. Of note is Syndrome's appearance near the end.
He's extremely polite when he finds a (more-than-slightly deranged-looking) Kari, and the whole conversation is one long CMOF.
Syndrome: Is this the... Parr residence?
Kari: HA-HA! YES! I'M KARI, THE BABY(twitch)SITTER!
Syndrome: Uh... Hello, Kari... I'm, ah...
Kari: YOU'RE MY REPLACEMENT! THANK HEAVENS YOU'VE COME! (beat) ...What does the "S" stand for?