Wario's home entrance in Mario Strikers Charged.
Wario in general.
" Butt smash!"
Gas Mask, and the opponents' reactions to it.
Mario: "Oh, Wario!" *cough*
Peach: "Phoo-wee! Wario!"
Waluigi has quite a palette: "Aw, Wario! *cough* Ugh, you're killing me!" "Aw, Wario! Hold it in!" And most of all. "Aw, Wario! Change your PANTS!"
Bowser Jr.: "BLEH! Wario!"
Even Wario has a few comments on it: "Excellent vintage," for instance.
Try using Bowser's Fire Storm on the resulting cloud of gas. This is what happens.
His frustration when the opponents get a goal. Particularly when he ends up wrestling the ball... and it blows up in his face.
"Keep throwing me!"
Even his Mega Striker is hilarious. He inflates like a balloon and blows the ball at the goal.
Daisy giving her team the evil eye after letting the opponent get a goal in.
Waluigi's crotch chop. He even has a variation where he starts to do it, only to wag his finger and say "ah, ah, ah!"
Boo attempting to do Waluigi's crotch chop despite lacking a crotch.
Bowser SETTING HIS TEAM ON FIRE for letting a goal in. Even funnier is that one of his teammates does the 'Stop, drop and roll' motion to put it out.
Peach sometimes gets angry at her team for letting a ball in and stomps up and down in a tantrum. While amusing in itself, Birdo's reaction is just comedy gold.
Petey Piranha just swaggin' after scoring a goal.
DK randomly punching the camera over in his exuberance.
Waluigi does the same thing out of anger, while telling the camera to say "cheese."
Luigi's Airplane Arms.
Yoshi chasing his tail is both funny and adorable.