Gintoki (yes, the man has so many hilarious moments, he needs his own section):
His attacks:
He managed to pull a Kamehameha move since he tried his best to practice it. But, not all the time. If he doesn't charge the stamina, he'll likely fail to perform it, and gets a short gust power instead.
Gintoki: Kamehame...haaaa....aaa?! D-Damn it!
His Thigh Burst kick, which he uses on Goku in one of the trailers. It's his Guard Break attack.
And for one of his specials he can also squirt soy sauce out of his Bokuto as well. The name is literally "Shouyuken" (Soy Sauce Sword).
He copies Seiya's stamina charging pose and even tries to recite his lines, but he can't remember them.
if the Kamehameha misfires: Humph. Go figure/That'll teach me to get my hopes up.
send an enemy flying or throw a Justaway: DONDAKE?!/No way!/ You gotta be out of your mind!
when riding a scooter: Where the heck did you get that?!/Did Gengai fix that up for you or something?!
Shinpachi's utter disbelief that Gintoki's bike is rocket-powered.note while riding the bike, press jump. The fact that it even double jumps is hilarious.
His Limit Break shows the credits, just like an anime opening/ending.
If you idle long enough while at low health, he'll try to get a quick sugar fix by scarfing a fruit parfait, which more often than not earns him a "DONDAKE!?" from Shinpachi.
Kenshin and Gintoki discussing the Shinsengumi :
Kenshin: The Shinsegumi I knew were noble warriors, men with whom you could stand toe-to-toe...
Gintoki: This useless policeman and I seem to be inseperable, unfortunately...
Shinpachi: Gin-san! That's the real thing, over there!
His answer to Lala's intro speech.
Lala: I'm Lala.
Gintoki: Have you said your piece now? Well, I'm sorry, but you went on so long, I didn't listen to half of it.
Shinpachi: ...Huh?! But that was only two words!
Goku's everywhere!
Gintoki: Ha ha! My name is Son Goku! Let's do this!
Shinpachi: Gin-san won't be happy!
Goku: Hey, you! I'm Son Goku, and I'm coming for you! Let's both give this everything we've got!
Bo-bobo uses Don Patch in a lot of his attacks, including as a shield and an explosive projectile.
Heck, the fact that Bo-bobo's in the game pretty much guarantees laughs.
The fact that Kuroko's assist is just passing the ball...somewhere in the distance as a distraction.
Not to mention the fact that Kuroko can be right next to you all of a sudden. Gintoki, Ryotsu, and Vegeta are one of the examples. Only Koro-sensei, Momotaro, Akainu, and Hinata doesn't seem to be surprised that Kuroko even be in this.
Taro's ultimate attack, which plops a giant Yokai head onto his opponents as his friends Mari and Gen freak out.
Freeze-Frame Bonus shows that there's a line at the right side of Gintoki's face for no reason at this part that looks out of place but is so hilarious.
Saiki: It's okay, you're just going through puberty.
In their special intro if on opposing teams, Ryotsu asks Raoh to let him enter Kokuoh into horse races for quick cash. Raoh is less than amused.
Medaka also has a special intro where she calls Kokuoh adorable, which causes Raoh to say that she's scaring Kokuoh — a nod to the fact that Medaka loves animals, but they don't love her back.
In the story modes, Hancock hired Gintoki to help her track down Luffy. At one point Hancock calls him a Stand and he wonders if she thinks he's Crazy Diamond.
One of the loading screen quiz questions in the English version asks you what Goku's power level is. The correct answer is "over 9000".note It counts because the famous line is actually a mistranslation; it's stated to be over 8000 in the original.
Another Dragon Ball Z trivia questions asks for Frieza's power levelnote His infamously boastful 53000. An incorrect answer given is 5.
Frieza and Ryotsu have a special intro if pitted against each other. Turns out Frieza remembers that one time Ryotsu tried to arrest him for illegally parking his spaceship.explanation to elaborate, this special intro references the Kochikame 30th anniversary special where Ryotsu did indeed try to arrest Frieza for illegal parking and none of Frieza's attacks killed Ryotsu leading to Frieza rage quitting the fight and leaving Earth in disgust.