Any time the first group bickers amongst themselves is usually good for a few laughs. The conversation/argument between Daniel and Sam/Aidan near the beginning particularly stands out, especially when he tries to lay down his "ground rules"
Daniel: Rule one: don't attack me. I don't care how much infighting there is, but I don't want to have to fight anyone.
Sam: So generous.
Daniel: Rule two: don't follow me around like a pair of braindead sheep. I am not going to be the leader of this little triumvirate of twits. And me.
The surprisingly calm way Bonnie reacts to growing something that looks more like it would belong on an arthropod or reptile then a human after cutting her arm off.
Bonnie: This healing thing is awful. I want a refund.
Daniel: Stop philosophising and get on with it. I need someone to share my pain of slowly lost humanity. Apart from Bonnie. She's decidedly unsympathetic.
Bonnie: Shut your face.
And let's not forget the begining of chapter two when Jacqueline exits her room, and we get our first glimpse of how the second group makes Sam, Aidan, Daniel, and Bonnie look harmonious.
Jacqueline: Is this place supposed to be on fire? What is this place? What is going on?
The little tussle between Bonnie and Clara about Your Other Left.
At one point the author was answering questions in character on their Tumblr, and some of them are just gold. Like when Device was asked about the weirdest thing he's ever seen in the Benefactors League.