A rather humorous moment in "Slammin' Sammy's Stunt Show Spectacular", a mysterious figure appears at one point in an attempt to sabotage KITT. When he can't get the hood open he attempts to do some damage from beneath the undercarriage. KITT wont have any of it and keeps pulling forward and back to mess with the guy until he decides to deflate his tires, while the man is still underneath.
KITT: If I were you buddy, I would get out of there before you get yourself trapped.
Saboteur: (gets out from underneath KTT's undercarriage and flees) Who said that!
KITT: (re-inflates his tires) Really, some people are too much.
When KITT played Matador against a bull in "Not a Drop to Drink". Also counts as a funny moment.
In "No Big Thing," Devon is arrested in a small town on bogus charges. He overhears his cellmates talking about breaking out and offers to come up with a solution, if they let him come along. When it's pointed out that he "doesn't look like the prison break type," he agrees— adding that the SS thought the same thing, which allowed him to break out of two German prison camps in the war. His cellmates agree, Devon executes the breakout and even works in a Dukes Of Hazzard leap with the getaway truck. This being a man who's, up to this point, been a very stoic, serious character.
The episode "Inside Out" opens with Michael and the crook he'd apprehended off-screen fighting inside KITT. Michael puts an end to the struggle by having KITT open the sunroof, and hitting the eject button.
In "The Topaz Connection", KITT manages to outpace a luxury private jet headed from Las Angeles to Las Vegas.
Once again Michael handles someone holding him at gun point in "Short Notice", this time by making excellent use of KITT's ejector seat.
Michael: How about some music?
Donny: You're a condemned man, you want music? That's fine with me.
Michael: (smiles, just before hitting the passenger eject button)
When Michael threatened that ass in "Junkyard Dog" after they pulled KITT up out of the mud. He was reallybadass.
KITT was pretty badass himself after his recovery and got over his fears and PTSD.
The end of the episode "Blind Spot". When Michael pulls the villain out of his wrecked car expecting him to try and fight back (like they always do). Instead, just as he pulls back for the punch... the guy falls over backwards. And the look on Michaels face says "that was rather anticlimactic".
Also counts as a moment of funny.
The finale of "A Good Knight's Work" When KITT slams into an apartment window... three floors off the ground.
April: You might as well know what I think about you and your destiny. Your destiny is to never have a woman who wants you for yourself. Never have a man who respects you for what you are. Your destiny is death. And if you don't believe me just go take a good look in the mirror. There are holes where your eyes should be. And behind them there's nothing but blackness.