Big Lipped Alligator Moment: The scene where Dr. Pretorius shows Dr. Frankenstein a bunch of small humans he's created and placed in jars.
Crazy Awesome: Dr. Pretorius (Ernest Thessiger) is a Camp GayMad Scientist who grows tiny people in jars, does not so much tinker in God's domain as wage open war against him, dreams of creating a race of monsters and interrupts his grave robbing to hold cozy picknics with Frankenstein's monster in the crypt. To put it another way, this is a Mad Scientist who scares Dr. Frankenstein.
Ensemble Darkhorse: Despite only having a short appearance at the end of the movie, the Bride has become one of the most famous Universal monsters.
Uncanny Valley: The Bride. She looks normal, except for her hair and a few scars, but the way Elsa Lanchester portrays her: the wide-eyed yet expressionless face, the stiff arms, the sharp, jerky motions of her head, the hiss ...
Values Dissonance: The hermit teaches the monster to smoke, saying it is "good". This film was made before the harmful effects of smoking were known.