Big Lipped Alligator Moment: The scene where Dr. Pretorius shows Dr. Frankenstein a bunch of small humans he's created and placed in jars which has no analogue in the original novel.
Crazy Awesome: Dr. Pretorius (Ernest Thessiger) is a Camp GayMad Scientist who grows tiny people in jars, does not so much tinker in God's domain as wage open war against him, dreams of creating a race of monsters and interrupts his grave robbing to hold cozy picknics with Frankenstein's monster in the crypt. To put it another way, this is a Mad Scientist who scares Dr. Frankenstein.
Ensemble Darkhorse: Despite only having a short appearance at the end of the movie, the Bride has become one of the most famous Universal monsters.
Ho Yay: The blind man and the Monster have their moments.
I Am Not Shazam: Lord Byron himself actually calls the monster Frankenstein in the prologue, and Pretorius is quick to dub the new female monster "the bride of Frankenstein". Although perhaps he was using the word "of" in the same manner as in "Monster of Frankenstein" (This may also be an artifact left over from the original plan to have Elizabeth's heart implanted into the Bride, which would have made the name literal.)
Uncanny Valley: The Bride. She looks normal, except for her hair and a few scars, but the way Elsa Lanchester portrays her: the wide-eyed yet expressionless face, the stiff arms, the sharp, jerky motions of her head, the hiss ... she actually makes the Bride less human than Karloff's Monster.
Values Dissonance: The hermit teaches the monster to smoke, saying it is "good". This film was made before the harmful effects of smoking were known.
The poor old hermit, who is so happy to finally have a friend, only to be separated from him almost immediately and having his home burned down shortly after.
The elderly couple at the beginning, who previously lost their daughter to the monster and end up losing their lives here because of the man's thirst for closure. Although he ends up being proven right when he tries to make sure that the monster is really dead, he ends up going by himself and puts himself in a precarious position for the monster to drown him like he did with his daughter. And then his wife ends up helping the monster get out of the sewer (thinking it's her husband) only to be flung down by the monster when she recognizes him.