- During the Monster's double murder at the beginning of the film, an owl is shown observing the event, reacting with a bored expression.
- The two goofy graverobbers working for Pretorius say at one point during their labors that, "Next time, I say we just let the cops hang us." That turns out to have been a bad wish to make as the Monster ultimately kills them both.
- Pretorius' reaction to the monster walking into his laboratory, greeting him as if he were a visitor.
"... Oh. I thought I was alone. Good evening."
- Frankenstein learning to smoke from the hermit.
- The Creature angrily trying shoo the fire from the hermit's match, which the hermit insists on doing so in spite of the growling.
- The homunculi scene, particularly the bit where the tiny king is trying to get to the tiny queen.