This is based on opinion. Please don't list it on a work's trope example list.
Tearjerker / Bride of Frankenstein
The couple that the monster kills at the beginning of the film were the same people who lost their daughter to the monster in the previous film. By this point, it's clear that their lives have been absolutely shattered by the monster's previous actions. The man is single-minded in his obsession with making sure the monster is dead, to the point of exploring the burning wreckage of the windmill by himself, and the woman pleads for him to stop, saying that nothing will bring their daughter back and that it would only hurt themselves further to continue the hunt. And then the monster reveals himself and ends up killing them both.