Original run (Seasons 1 and 2, and FWWM)
- Virtually everyone's initial reaction to Laura's death. As hammy and bizarre as they were, the complete and utter despair this town felt at her loss is unmistakable.
- The school announcing her death over the loudspeaker, and Donna bursting into loud, hysterical sobs in the middle of class.
- The shot of the telephone's receiver dangling, from which Mrs. Palmer can be heard reacting to just learning of Laura's death.
- The entire sequence from the discovery of her body is haunting, as Mrs. Palmer lives the worst nightmare of any parent during the fruitless search for Laura's whereabouts.
- Andy's phone call to Lucy telling her that they found the rail car where Laura was tortured and murdered.
Andy: [breaking down] Tell Harry we found where... where it happened. Where Ronette and Laura were taken and tortured. Tell Harry... I didn't cry.
- Harold Smith being Driven to Suicide following his feeling betrayed by Donna's snooping.
- The fallout from Maddie's death, seemingly felt by everyone in the Roadhouse — Donna (who was never particularly comfortable or pleasant around her) breaking down into tears, Bobby (who never even met her) looking confused and grieved at the bar, Cooper being comforted by the waiter and obviously feeling his failure to stop BOB.
- Leland's death. Both tears of sadness, and, due to him dying as himself and seeing a vision of Laura, not to mention Coop guiding him, tears of bittersweet happiness.
- Josie's death (or, rather, her ambiguous Fate Worse Than Death), and Truman's Heroic B.S.O.D. that resulted from it.
- Donna discovering that she's really Ben Horne's daughter eventually causes her to break down crying. Made slightly more heartwarming when it causes her to loudly affirm to Doc Hayward that he's her daddy and then turns to slight Nightmare Fuel when Doc Hayward gets so angry with Ben Horne that he nearly kills the man.
- The last scene of Fire Walk With Me.
- Most of Fire Walk With Me is tear jerker material due to the story alone: a young girl living under constant abuse and doing anything she can to numb herself to it, even though we know from the premise of the series alone that this is a doomed character.
- As Season 3 starts, it's made apparent that BOB has still been in control of Cooper's body for the entirety of the time skip, leaving him in the Black Lodge this entire time. What's more, according to Andy and Lucy, most of the main cast hadn't even seen him for 24 years, meaning BOB disappeared with the body soon after the Season 2 finale. While this is more played for Nightmare Fuel in the episode itself, it's incredibly saddening to know that poor Cooper has been powerless for two and a half decades.
- When we see Cooper still in the Black Lodge, he is near completely apathetic to all the bizarre events occurring around him. This is further emphasized by the blank, empty stare he maintains the entire time. Cooper really has become an Empty Shell
- Also in the beginning of season 3, a clearly ailing Log Lady telling Hawk that she's too weak to come with him on the quest her log sends him on. Made worse by the fact that Catherine Coulson died shortly after filming the scene.
- Doublely so in her last message to Hawk in Part 15 where she speaks her final words to him after telling him she's dying. When Hawk later breaks the news to the rest of the Sheriff team, the emotion on their faces just shows how much her life meant to all of them.
- A brief scene shows that Sarah Palmer's drunkenly spends her nights watching TV, clearly looking heartbroken.
- When Cooper finally makes it out of the Black Lodge via switching with Dougie Jones, he becomes more of an Empty Shell, not even remembering who he is or even basic human function. It's depressing to see Cooper came out like this.
- In Part 4, Bobby enters the police station's conference room, where Hawk has been doing some research related to the Laura Palmer case. All it takes is one look at Laura's old Homecoming photo for Bobby to break down in tears, as if everything from 25 years ago is hitting him all at once. We even hear Laura's theme for the first time in 25 years to really drive it home.
Bobby: (trying to compose himself) Man...brings back some memories.
- As of this writing, seven episodes have aired. A staggering five have featured "In Memoriam" cards in tribute to cast members who died both before and after the filming of this season.
- Diane's breakdown after visiting Doppleganger!Cooper. Up until the visit, she was rash and showed nothing but contempt for everyone in the FBI. It's not-so-subtly implied that she was raped by him.
Diane: It's his heart that's missing.
- Doubling as a Crowning Moment of Heartwarming, Part 11 reveals that Cooper, as Dougie, was the difference between life and death for a poor homeless woman at the casino - her single interaction with him caused her to regain her home, family, and money and saved her from her almost certain demise. And this whole time you thought that her entire character was just meant as a comic gimmick.
- Poor Shelly has to watch her daughter stay in an abusive marriage, just like she stayed with Leo.
- In Episode 6 when Richard Horne hits the little boy killing him and pulls over later only to wash the blood from the car. While the mother is sobbing clutching the kid.
- Part 13 reveals that Ed and Norma did not end up together. Norma has moved on to dating some business man who's helping her expand the RR Diner, while Ed is implied to have lost Nadine as well. While most episodes end with a musical act at the Roadhouse, this one just shows Ed sitting quietly alone at his gas station. Although this is mitigated in Part 15.
- The final scene — followed by the offscreen death of — the Log Lady in Part 15. When Hawk tells them of her demise, everyone in the conference room promptly goes into mourning. Especially sad, seeing how Catherine E. Coulson died in real life.
- In Part 16, Diane vividly recounts the night when Mr. C raped her. Made worse when it's revealed that Mr. C took her to the gas station to have her replaced by a doppleganger.
- After Dale returns to himself, he says goodbye to Janey-E and Sonny Jim. While Sonny Jim pleads with Dale not to leave, Janey-E realizes that Dale isn't Dougie and tearfully says goodbye to him.
- In Part 17 Dale steers Laura away from the path to her murder by taking her hand and guiding her through the forest. As awesome and heartwarming as that is, it doesn't last long. Dale turns back and finds his hand empty, then hears Laura screaming in the distance. He stares off into the forest, silent and alone.
- Part 18 becomes the ultimate Tear Jerker in part due to the Fridge Horror that sets in once you take the time to think about all the implications it raises. In trying to Set Right What Once Went Wrong by attempting to save Laura from being murdered in the past, all Cooper ends up doing is creating an alternate world where everything seems a little emptier, a little darker. Laura in her new life as Carrie Page is now some sort of fugitive on the run with the only clues to her current situation being the rotting corpse in her house when Cooper goes to collect her. The Palmer family may not even exist here, and if they don't, it's possible many of the other precious people in Cooper's life may have undergone a similar fate. Even the Double R diner which has always been shown to be open no matter how late appears dark and abandoned when Cooper and Laura/Carrie drive into town, further foreshadowing that not all is right in this new world. It's only when Cooper discovers that the Palmer family doesn't live nor have they ever appeared to live in the new Twin Peaks does he realize something is horribly, horribly wrong, and the weight of his actions finally set in: he's been separated again from everything dear to him and Laura by rewriting reality, and has no idea what to do next or if there's even a way to fix things.
- His reaction to discovering Sarah Palmer doesn't live in her house is the first nail in the coffin. When he's told this news by the current owner of the house, all Cooper can do is ask her to go down the chain of previous owners in the hopes that one of them might be Sarah or anyone familiar. It's as heartwrenching as it is unnerving to see the normally capable, always-ten-steps-ahead Cooper caught off guard and trying to grasp for whatever straws he can get to salvage the situation.
- At long last, we finally see Cooper and the real Diane reunite for the first time ever in the series and it's nothing short of heartwarming, especially following the revelation that they're intensely romantically attracted to each other. Then she disappears after crossing over into the new reality with him while Cooper is left with nothing but a note from an unknown woman named Linda addressing someone named Richard that goes into detail about their ruined relationship and how Linda wants nothing more to do with Richard. Considering the eerie vision Diane has the night before of a woman who looks just like her and the new, unpleasant personality Cooper begins to slowly exhibit the longer he stays in this reality, it's very likely that Richard and Linda are this world's versions of Cooper and Diane, and Diane didn't disappear: her identity was supplanted by Linda's and she forgot herself and Cooper.
- This makes Cooper's final fate even worse. He hasn't just failed in his quest to truly save Laura; he also ended up losing yet another beloved woman in his life whom he was deeply in love with who now wants to forget about "him" due to the effect of his otherworldly meddling. And there's a strong implication what happened to Diane is slowly happening to him. Not only has Dale Cooper completely and utterly failed, but he may also cease to exist altogether.
- It should be noted, however, that the Gainax Ending is ambiguous enough that it can be taken in other, less bleak ways.