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Tear Jerker: Tangled
The scene of the king, right before he and the queen release the lantern on Rapunzel's 18th birthday, is enough the make even the most heartless scrooge shed a tear. The fact that there's no dialogue just makes it even worse.
It's the look of sheer pain on the man's face. That scene didn't need dialogue.
Flynn's heroic death at the end! Good lord, that is easily one of the saddest death scenes in the Disney Animated Canon. The expression of pure anguish on Rapunzel's face seals this particular scene.
Not to mention the absolute brokenness of her words when she sings the Healing Incantation.
The last, whispered/sobbed 'What once was mine' clinches it.
Just the audio is tearjerking. On the soundtrack, the song at this part is "The Tear Heals". They leave in the singing. It is absolutely heartbreaking. You can hear the pain.
The visuals themselves, or rather the details put into Rapunzel, are heart wrenching. Just take a close look at her face and you'll see that her eyes are bloodshot. Just a small detail but...argh!
The wide shots, too. Unlike the rest of the film (which is bathed in bright golden colours) it's bleached out, grey and dull. Rapunzel is alone with Flynn's body and a great rope of what's now her dark, ordinary severed hair. All the colour has gone out of her life.
Or when Rapunzel tell Mother Gothel that she will never stop fighting her... unless she lets her heal Eugene.
Also, the heartbreaking look on Rapunzel's face when she is led to believe Flynn betrayed her.
Flynn's face when he realizes where Rapunzel is. And worse once he's at the tower and thinks she's gone.
"You were my new dream." "You were mine."
An example of a happy tearjerker is the entire lantern scene.
After Flynn has been stabbed by Mother Gothel, Rapunzel tries to heal him using the Healing Incantation, and she starts to cry. Her tears fall on Flynn's wound, healing him.
What happens right before it as well: Flynn/Eugene has a chance to survive, but sacrifices his life by cutting off her healing hair so Rapunzel could be free from Mother Gothel.
When Rapunzel sees her birth parents again.
The fact that Rapunzel lets out that tiny little gasp before their big embrace seals the deal.
When taken out of context as a spell incantation, "Healing Incantation" is a very sad song; it's basically the pleas of someone who desperately wants to escape fate with "change the Fates' design" to "heal what has been hurt," "save what has been lost," and "bring back what once was mine."
And then eventually, it's not so out-of-context.
The scene where Rapunzel and Flynn are trapped in the sealed cave with rapidly-rising water, thinking they're about to die. Partly because of Rapunzel's tearful apologizing and "Mother was right; I never should have left..." and partly because of Flynn trying to tell her it's hopeless a few seconds earlier.
Flynn: "It's no use! I can't see anything!"
*Rapunzel panics for a second, then dives underwater*
Flynn: "Hey! Hey!" *pulls her out, holding her shoulders* "There's no point!'' It's pitch-black down there..."
The look on Rapunzel's face when you-know-who falls out the window, to their dusty death?
It's heartbreaking, the way Rapunzel still reaches out her arms when Mother Gothel dies. Regardless of everything that has happened, she's still the only mother figure Rapunzel has ever known.
To the more cynical viewer, the true tragedy is the fact that even though Rapunzel has witnessed just how horrible Gothel is, she still irrevocably loves her. It's a sign of how effective Gothel's "brainwashing" really was.