In an issue of The Ultimates, there is a wall of photos of everyone that died in the Hulk's rampage. In itself, it's a really sad moment but seeing a child's drawing of a policeman with the child's handwriting on it with the words "We miss you Daddy" clinches it.
There are many Tear Jerker moments in Runaways but Gert's death takes the prize.
What makes it worse is it is the second time Gert has died in Chase's arms.
The girl's design was based on a story in a pre-code horror comic, trust me that was a real tear jerker.
She shows up again in the sequel by sneaking into Sheldon's hospital room.
The death of Gwen Stacy from an entirely different angle. It's not less painful than the original story.
The Death of Captain Marvel. Mar-Vell telling his lover, Elysius, that's he's dying without dialogue. When the Skrull general salutes Mar-Vell as an honorable enemy, even while his own people, the Kree, reject him. And finally, when Thanos comes to reconcile him to death.
Hang on until Uncanny X-Men #522, in March. That is all.
Silver Surfer: Requiem. The story of the four-part "what-if" focused on the Silver Surfer as he discovered he was slowly dying because the power that Galactus gave him started wearing off. It's a wonderfully touching series, with a tear-jerker happening at least once in each and every issue:
Issue 1: The Surfer discovers that he is dying, with Reed Richards breaking the news to him.
Issue 2: Spider-Man, the last person he encounters before beginning his journey home to Zenn-La, convinces the Surfer to unleash the Power Cosmic long enough to give the world a glimpse of the same freedom and peace he's experienced thanks to the same power.
The only thing he ever wanted was to bring peace to the world. And for five minutes, he did just that.
Issue 3: On his trip back to his homeworld, the Surfer becomes stuck in the middle of an interplanetary war that had been waged for generations, with both sides' leaders now simply perpetuating the battle for their own gain. Norrin disables the ability of the two sides' fleets to make war, then shows both sides the truth of their situation. His intervention results in the end of the war and a peace between both worlds.
"If sacred places are spared the ravages of war, then make all places sacred. And if the holy people are to be kept harmless from war, then make all peoples holy."
Issue 4: Norrid Radd returns to Zenn-La to face his fate, and Galactus - who returns to offer the Surfer a chance to live, which Norrin rejects - tells him that no harm will ever come to Zenn-La, respecting the final wish of "the most honorable being I have ever known". After the Surfer passes away and the planet has mourned his loss, Galactus sends his remains into deep space and creates a new star using his power, one which would remind Zenn-La "not so much of his sacrifices, not so much for what he lost, as what he gave."
The death of Marvel Comics villain Black Knight. On his deathbed Nathan Garrett laments that he wasted his life and his intellect by turning to a life of crime and asks Dane Whitman, his nephew and the only person who ever meant anything to him, to use his technology for good so that the world will one day remember the Black Knight as a benefactor to mankind. Dane accepts.
When the Irredeemable Ant-Man tells the mother of his child that he can't be involved in its life, as well as the whole exchange with the empath he falls in love with. That final speech where he admits his failings and promises that he will honestly try to change, even as it becomes apparent that he probably can't and never will.
The Punisher MAX series has more than a few emotionally hard-hitting moments, such as the final couple of panels of "The Slavers" in which we learn that, despite Frank killing the titular slavers and Viorica being able to assume the closest thing to a decent living in New York, the scars from her mistreatment and the killing of her baby will never fade away. Also, the end of the fifth issue of "The Man of Stone" arc, where O'Brien dies in Frank's arms, asking only that he kill Rawlins for her, and to stay with her so she wouldn't die alone.
Iron Man is not exactly thick with these moments, but there is one that always gets me: In #22 of the first volume, Janice Cord, Tony Stark's love interest of several years, gets dragged into a three-way battle between Iron Man, Crimson Dynamo and Titanium Man. As she dies her fading last words are "You called me 'darling'... strange... it sounded so much... like the way Tony says it to me...". She never even knew. And the superhero business was definitely not her world. She was practically the definition of an innocent bystander.
The last issue of perpetually B-list Marvel hero Quasar's solo series. Thanks to various cosmic events, his girlfriend has been sealed away on an alternate Earth that she can never leave and he can never visit (as ordained by The Living Tribunal, essentially God's personal representative). His security company is on the verge of collapse. And one of his nastier villains escapes his other-dimensional imprisonment and threatens everyone he loves, causing Quasar to pull a Faking the Dead to his friends and family, and self-exile from Earth. Specifics change, but his overall happy level has barely fluctuated much from this degree of suck right to the present day (he's currently stuck somewhere between alive and undead, though this at least looks like it might change soon).
In the last issue of the mini-series The Thanos Imperative we have Mistress Death walk silently away from a distraught Thanos, who has just destroyed a whole universe to please her and prove to her that he loves her. As she walks away, a human-faced death sheds a tear, as she knows that he will forever be kept alive and never be hers to love. Something about Gods and such never being able to love is especially sad.
Fantastic Four #587, The Last Stand of Johnny Storm. the Human Torch, Ben Grimm, and the Future Foundation go through a portal to the Negative Zone to try to stop the Annihilation Wave. Johnny's niece, Val, knows that the only way to close the portal is for one person to stay behind and close it manually. Ben, who has taken a formula that makes him human for a week, volunteers to stay behind. However, Johnny pushes Ben through the portal back to Earth, sacrificing himself. The part that really makes it is Johnny's pure defiance and bravado until the very end.
Human Torch: A billion to one... you think I'm afraid of that? YOU THINK I'M AFRAID OF THAT? FLAME ON!
The aftermath is even worse, especially the reactions of Franklin and Valeria. They're just kids, but because of their powers and intellect they think they could have prevented Johnny's death. Franklin confesses to Spider-Man that he thinks he could have saved Johnny if he had a better handle on his Reality Warper powers. Spidey tells him he can relate, since he also failed to save his uncle. Valeria's way of dealing with it is both sad and disturbing. She writes up an agenda for the Future Foundation with Annihilus' assassination as a top priority. It's sad because she can't grieve like a normal little girl. It's terrifying because little girls should not be planning assassination attempts.
The worst of all though is how the ever loving blue eyed Thing mourns. He meets with Thor and Bruce Banner and the two try to show him a hologram of Johnny. Ben grows furious and attacks Thor. Thor is about to fight back when Banner stops him and transforms into the Hulk. The Hulk stands completely still as he lets Ben punch and attack him until The Thing tearfully collapses. For two characters defined by looking like monsters, this show of utmost humanity is absolutely gut wrenching.
Then there's Fantastic Four #267. Sue's pregnant with her and Reed's second child, but radiation due to exposure to the Negative Zone is threatening both of their lives. Reed calls in the experts in radiation - Bruce Banner, Walter Langkowski (Sasquatch of Alpha Flight), and Michael Morbius, but they're not enough. Langkowski suggests bringing in Otto Octavius, who's currently locked up and on meds, and when Reed goes to retrieve him, Otto is only too happy to once again be given a chance to help people instead of hurting them. Unfortunately, though, Reed just happens to drive the Fantasticar past an anti-Spidey billboard from the Daily Bugle, which sets Doc Ock off, and he jumps ship, forcing Reed to battle him. Reed eventually gets Octopus to settle down and agree to help again (though still in full Doc Ock persona), and they rush to the hospital, where Sue is saved, but... the final page, one panel enclosed in black, tells it all.◊
In The Mighty Thor #4 (Matt Fraction's run), Odin and Galactus and their respective forces are fighting over who will gain control of the Life Seed. Instead of fighting head-on, Odin and Galactus engage in a psychic battle where they show each other their worst memories. Towards the end of the issue, we see Galan, a younger Galactus, investigating the impending doom of his universe. The kicker comes when it is revealed to the reader and to Galan that his wife/lover is pregnant. For a brief panel, the reader sees Galan overjoyed at the prospect of being a father, only to have his universe, his lover, and his unborn child die a few seconds later. This one memory overwhelms the mighty Galactus. I'm not one for recons, but this one was rather touching, if only for the reason that it showed that Galactus, The Devourer of Worlds, was once a normal person who had everything taken away from in a brief instance; it really hits home when you realize that he has to kill trillions every year just to keep the current universe from being completely being destroyed.
This builds on Taskmaster's miniseries in which his origin is revealed. He was just a normal SHIELD agent who underwent a procedure to be able to mimic the fighting styles of anyone. Unfortunately, this had the side effect of making him lose his memories over time. Even worse, his wife (likely Finesse's mother) continues to operate as Taskmaster's handler in the hopes of keeping him from doing anything too evil (and reporting information he gathers to SHIELD to help protect him). Because even if he can't remember her, she still loves him (and, when he regained his memory of her briefly, he still loved her). The real tearjerker: The reason Taskmaster is a villain is because, while he can't remember it, he still feels horrible guilt over forgetting his wife because one of her earliest memories is being abandoned by her parents. This, along with Taskmaster's role in Initiative and Agent X largely make him one of the biggest woobies in the Marvel Universe.
Captain America discovering that Bucky is alive, but that he's the Winter Soldier. When he calls out to him and the Winter Soldier asks who Bucky is, and then when Steve restores his memories and Bucky remembers everything that he's done—and he tells Steve that he should have killed him instead.
It's probably best summed up by a quote by Ed Brubaker:
Cap still lost. If I was going to take away the tragedy of Bucky being killed in action, I had to replace it with something worse. Cap couldn’t save Bucky and because he couldn’t, Bucky became his own worst nightmare. And then in trying to save Bucky again — by giving him his memories back — Cap tortures Bucky by making him realize everything he did as the Winter Soldier too. Bucky is such a great tragic character and that tragedy has different sides to it now."
One issue of Punisher War Journal double whammies, especially with the last pages if you hadn't heard of what happened. The entire story isn't about Frank Castle, but about a normal joe named Ian, whose apparently a member of the NYPD. A supervillain ends up causing a ruckus and Ian holds him at gunpoint, ready to take him down. However, as the story progresses, you come to find out why he became a cop and why he was here now: he became a cop following the 9/11 attacks, but he wasn't a full cop, just an auxillary one. However, everything fell apart when his family had been killed in the Stamford incident and was let go. He put on the uniform and patrolled New York City not out of duty, but because it was all he could do to keep himself sane. When the Punisher shows up, giving the cops the opportunity to take down the villain, Castle goes to walk away, only to look up and see the death of Captain America. Just his stunned reaction to it - No. Not him. "No..." - is enough to see that the man has some great Hidden Depths and even he can be shaken by something like that.
The entire last issue of Garth Ennis's Fury: My War Gone By series is basically an examination of how little Fury's life of war has meant. Even the enemy who defeated him in Vietnam acknowledges that it was all pointless.
Fury: "I wanted the girl and the war and the victory. I chose one."
Maria Hill's backstory, as revealed in Invincible Iron Man. Her mother died either during or shortly after her birth, her father hated her for it, and she grew up with everyone telling her that she was terrible, from her childhood, to being kicked out of high school, to when she was in the Marines, to when Fury was yelling at her. On top of this, she's shown being treated badly by the S.H.I.E.L.D. agents around her, with one saying "so long, sweet cheeks" and her narration says that she can't resist slapping back once more. Is it any surprise that Maria's first instinct is usually to attack people? She's spent her entire life being hated for things as simple as being alive and being a woman?
Even worse—we see a photograph of Maria's desk when she's clearing it out - it's of her shaking Fury's hand. This implies that she used to look up to Fury, but in another issue where he broke into the Helicarrier to warn her about the Skrulls, he didn't even remember meeting her before.
There was one 9/11 short that was particularly memorable for being tragic and heartwarming at the same time. Basically, both well known heroes and villains (magneto, Dr. Doom etc.) put aside their differences to help clear the rubble and help search for bodies. One particular scene has a closeup of Dr. Doom's mask, showing us that he's CRYING. The most memorable scene for this editor however was one where a little boy is sitting among the rubble. When Spiderman asks him if he needs help, the boy responds matter-of-factly that he's waiting for his father, only to see his father's corpse being carried out of the rubble. His reaction is summed up in one word: "Daddy!!" This editor can't even think about this scene without crying a lot.
Mariko's death and Morph asking the Timebroker to let him stay on the team even when he's allowed to go back his original reality, which we've seen is pretty nice.
Mimic's death at the hands of Proteus, dying like a dog being mocked until his last moment, and Blink trying to save him, trying to believe he's somehow still alive even as other characters spell it out explicitly. That arc ended on a major Downer Ending, especially after what happened to Morph.
1985. Just the whole mini series. It was a beautiful, wonderful ending - but Gosh, it was soooo saaad.
In Powers: Bureau, Deena and her then-partner get some Powers semen spilled on them. He ends up dying as a mutant Enfant Terrible rips out of his gut, but Deena is cleared by a doctor. But when she and Walker later catch the guy who was dealing the stuff, he spooks Deena into going back and seeing the doctor again. So she does, and is very surprised to find out that she's now pregnant. She doesn't tell anyone, but when she's later physically attacked by another Power a few weeks later, she ends up losing the baby. Christian takes her home and asks why she didn't tell him she was pregnant, and risk dying like her former partner, and she replies "Because...I really wanted it." The issue closes with Deena, who's rarely shed a tear throughout the series, crying into her pillow as Christian tries to comfort her.
Daredevil arc "Return of the King" has the murder of Wilson "The Kingpin" Fisk's adopted family by Lady Bullseye. Kingpin's realization that no matter how far away he might run, his life will always find a way to drag him down is heartbreaking.