Referenced By: The Powerpuff Girls
- In The Simpsons episode "The Dad Who Knew Too Little", Homer and Lisa are framed for a crime and go on the run. They use a black hair gel to disguise themselves and Lisa comments she looks like a Powerpuff Girl.
- The MAD sketch "2 Broke Powerpuff Girls" is a mix of this show and 2 Broke Girls. Tara Strong and Tom Kane reprise their roles of Bubbles and HIM.
- The show has been referenced twice in Robot Chicken
- A three-second sketch has Professor Utonium say "All thanks to stem cell research", with the girls concurring with him.
- Another sketch shows what happens if cocaine was added into the ingredients to create the girls.