- The level with Eely-Mouth. After you clean his teeth, he spits out his golden tooth (actually a Shine Sprite) and leaves behind a heart made of coins. Eely-Mouth is saying thank you. Awwww.
- When FLUDD seemingly passes away, Mario not only looks depressed but he also seems to be wiping away tears from his eyes. F.L.U.D.D may have simply been a robot with artificial intelligence to most people but to Mario he was a true friend.
- The scene with Bowser and Bowser Jr. at the end, oddly enough. You can actually see a tear forming in Bowser's eye when he's trying to break it to Junior that Peach isn't his mother.
Junior: Someday, when I'm bigger... I wanna fight that Mario again!