When Anna of all people watches another good man fall, and full of righteous fury tries to pick up a gun and blast the Predator. Remember that just a few days ago she was fighting these guys herself! It's nice to see she'd grown fond of them, and a little tearjerking at the same time.
The Video Credits at the end, with the cast all smiling at the camera. It's a little silly and totally out of place after the Bittersweet Ending, but somehow incredibly cute.
In Predator 2, Harrigan's award of a 18th century flintlock pistol—possibly taken from a past worthy prey?—qualifies.