Gru has several, but an especially noteworthy one is when he breaks into Vector's Fortress in an attempt to get Margo, Edith and Agnes back from him. This involves jumping over and around a series of heat-seeking missiles and then punching a shark in the face. With an Offhand Backhand.
Gru: Listen close, you little punk, when I get in there, you are in for a world of pain.
(Vector laughs it off, thinking that Gru is just blowing hot air... until Gru punches the camera hard enough to crack the lens).
Agnes: (smugly) He's gonna kick your butt.