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9* At one point in the ''Manga/AhMyGoddess'' manga, a botched love potion fed to Keiichi causes any female to meet his eyes to become passionately infatuated with him -- starting with his sister Megumi. She says, "Our love can transcend these foolish bonds of blood!" His horrified reaction: "'''Oh no it can't!'''" Fortunately, neutralizing the potion [[LaserGuidedAmnesia wipes her memory of the incident]].
10** As you can [[RuleThirtyFour easily guess]], there are {{hentai}} dōjinshi expanding on this incident.
11** The anime-remade episode has this too, and is more outrageous, where Megumi goes to the length of clinging onto Keichii and fighting over him with Skuld, Urd and Peorth who also fell victim to the effect of the potion.
12* Minato and Juunichi's relationship in ''VisualNovel/AkaneIroNiSomaruSaka'' [[LampshadeHanging is seen as this by everyone]] despite the siblings' insistence that it's not. It turns out everyone else was spot on, because when the show ends, Minato is the one who ends up winning. Interestingly enough, the level of incest between Minato and Juunichi changes depending on the version of the story. They're fully blood-related in the original PC game, but Minato is adopted in the [=PS2=] version and possibly in the anime as well.
13* One of the main drives of ''Manga/AkatsukiNoAria'''s story is how Aria Kanbara and Natsuo Nishimikado are half-siblings, what with Aria being an HeroicBastard, but are ''very'' attracted to each other. [[spoiler: it turns out that [[NotBloodSiblings NOPE]], they aren't related, and later Aria has to search for her actual birth father.]]
14* The soft core {{Hentai}} manga, ''Aki Sora,'' has older sister Aki and her younger brother Sora engage in an affair after discovering a mutual physical and emotional attraction for each other. He also has an affair with his PsychoLesbian [[{{Twincest}} twin sister, Nami,]] after she rapes him as payback for banging her crush. Later on it's revealed that their father left years ago because of [[DaddyHadAGoodReasonForAbandoningYou the scandal of having sired the three siblings with one of his younger twin sisters.]] While he does take Aki away for the two's own good, the manga ends with her and Sora reuniting, [[MaybeEverAfter implying they have a chance for a happily ever after]].
15* ''Manga/AngelSanctuary'':
16** Setsuna worries that this relationship will make Sara's life difficult, but their feelings are mutual. Even though both of them are consistently faced with opposition (whether from human society or from Heaven), [[spoiler:their love never falters.]] They do, through the course of their separate adventures, mature and become aware of the sacrifices their allies are making to help them, and the impact their actions have on the people around them. In the end, [[spoiler:they are reunited in Assiah (Earth), they go back to their human lives, still siblings, and Setsuna is sure they'll find a way to make it work.]]
17*** The OVA ends much earlier, and with Sara dead and Setsuna killed so he may go to the afterlife to rescue her, but it abruptly ends right there, leaving it ambiguous if he'll succeed, but given they reference the story of Orpheus... high hopes are not had.
18** In Heaven, all close relationships are taboo, and all angels are related. But, with the exception of twins, they don't tend to think of each other as siblings. Though the dynamic is different, it still applies because if any of them have children together, genetic mutations are a valid concern.
19* The novel adaptation of ''Literature/{{Baccano}}'' says that [[HeroWorshipper Graham Specter]]'s first love was his sister. No information has been given beyond that so far.
20* Subverted in ''Literature/BakaAndTestSummonTheBeasts'' because while Akira Yoshii really wants to move her relationship with her younger brother to the next level, the normally super-horny Akihisa surprisingly expressly considers incest to be disturbingly immoral and constantly resists the nonstop attempts at seduction of his older sister.
21* ''Baku Ane: Otouto Shibocchau zo!'': A guy's four sisters become his harem.
22* ''Anime/BattleSpiritsShonenGekihaDan'' has Yuuki and Kajitsu Momose. From the beginning, it seems apparent that their affection for each other goes beyond the norm for a brother and sister, especially given that this is a kids show. The truth is that [[spoiler: the two were reincarnated lovers who unfortunately came back as brother and sister. Which didn't stop them from loving each other, as they were to be married in their previous life. Both die, and then re-incarnate without the blood relation. However, because they can't catch a break, this time they're stuck with InterspeciesRomance.]]
23* Kyouka from ''Manga/BGataHKei'' is admittedly in love with her brother, wants him to take her virginity, and even has a StalkerShrine devoted to him. He's oblivious. Eventually, [[spoiler:when she gets a better understanding of what sex entails, she decides she never wants her brother to do something so dirty, and gives up on him]].
24* Hansel and Gretel from ''Manga/BlackLagoon'', even though [[spoiler:it's not clear which of them is which, or if they're even different genders]].
25* ''Manga/{{Berserk}}'' subverts this, since [[spoiler: Serpico and Farnese were in love at one point and are half-siblings, but Serpico's love becomes more brotherly after he learns that Farnese is his half sister.]]
26* It's questionable in ''Manga/BirdyTheMighty: Decode'' due to the fact that both are genetically-engineered {{Super Soldier}}s, but [[spoiler:given Nataru was taken from a lab called "Cephon"; for all intends and purposes, the titular Birdy's full name is "Birdy Cephon"; and neither seem to be aware of it, it's possible their DNA is similar enough to count as brother and sister. They're also attracted to one another.]]
27* ''Bloods: Inraku no Ketsuzoku 2'': The Sagemiya family is cursed in that its members are sexually aroused in the presence of another of the same bloodline. Misaki twin brother Shun are both disgusted over this and have sworn to resist the curse. However, Shun loses his composure and starts banging their big sister Kagu. After Misaki finds out, she too gives in to the curse and begins an affair with Shun.
28* The sum of the plot in ''Boku wa Imouto ni Koi wo Suru'' ("I'm In Love With My Little Sister.") Talk about ExactlyWhatItSaysOnTheTin.
29* ''Manga/BusoRenkin'': {{Subverted|Trope}} with the Hayasaka twins who have quite a bit of IncestSubtext when they are first introduced, which is latter explained when their backstory reveals that [[spoiler: they were kidnapped as children and their kidnapper/surrogate mother would have them play game built around wedding vows to build a strong bond between them. When their real parents rejected them, the two legitimately felt like they only had each other, and latched onto the one thing that was happy from their childhood.]] Once they realize that they're not alone, a lot of the creepy subtext gets toned down, and there are hints that Oka may have a crush on Kazuki.
30* In an unusually negative scene, there is a pseudo-incestuous rape sequence in ''Manga/CeresCelestialLegend''. It also contains a subversion and plot device of this trope, seeing as [[spoiler:Shisou Mikage, who is still madly in love with his wife Ceres, is reincarnated as Aki Mikage. Aki's twin sister Aya is the reincarnation of Ceres, and she is eventually led to kill him to save both herself and Ceres. Aki and Aya themselves ''do'' love each other greatly, but ''not'' in an incestuous way]].
31* ''Anime/CandyBoy'''s main theme is the yuri subtext between (fraternal) twin sisters, Yukino and Kanade. The lyrics in the recap video appended to the first episode proclaim that "this may be the best pairing in the history of the world".
32* Due to technicalities regarding reincarnation in ''Manga/CardCaptorSakura'', Syaoran is, technically speaking, Sakura's half-brother (although they don't know that until ''very'' late in the series). [[spoiler: Sakura's father is a reincarnation of Clow Reed, albeit one without any known magical abilities, and Syaoran is one of Clow Reed's descendants, on his mother's side.]] This being a series by Creator/{{CLAMP}}, it doesn't ''really'' matter, or even factor much (if at all) into the plot.
33* In ''Literature/ChivalryOfAFailedKnight'', Ikki's younger sister Shizuku has deliberately ''chosen'' to go this route. Incensed by the [[TheUnfavorite neglect and abuse]] Ikki suffered at the hands of the Kurogane family which finally forced Ikki to run away, she has decided to love him in every way possible to make up for the other members of their family. This includes "as a lover", in her own words, which causes quite a few raised eyebrows around campus. Ikki, to his credit, finds this highly disturbing and other characters note that her behavior is taboo.
34* ''Manga/{{Citrus}}'' chronicles the growing romance between two stepsisters.
35* In ''VisualNovel/{{Clannad}}'', Tomoya's wild imagination causes him to imagine the Sunohara siblings as this when he [[https://www.youtube.com/watch?v=fEn7Magci80 misinterprets one of Mei's lines]].
36* Happens quite frequently (both within the story's present time and in flashbacks) between [[spoiler: Michel]] and [[spoiler: Coco]] in the manga ''Manga/CopernicusBreathing''.
37* In ''Literature/{{Corsair}}'', Jean-Hughes saves his seven-year-old brother Canale from being raped, but he can't stop imagining himself doing the exact same thing to him. This leads to him pinning the guilt and blame on Canale and loathing him even more.
38* Cain Hargreaves, the lead of the ''Manga/CountCain'' manga and its sequel series ''Godchild'', has this as part of his origin story. Turns out part of why he has the family curse so bad/why his parents always acted so weird with him/[[ArtisticLicenseBiology why he has]] sort of golden eyes, is that his mother isn't his mother. His father's crazy sister, Augusta, is. When he finds out and goes to see her in her asylum, she jumps to her death in front of him.
39** For some reason, Kaori Yuki [[FanonDisContinuity decided to reveal]] in the end that MagnificentBastard Alexis was actually manipulated by his much more evil sister Augusta because... she was just that evil, apparently. Yeah, evil enough to seduce her brother into impregnating her and then spend the next twelve years catatonic in an asylum before killing herself just to traumatize the kid. According to her ghost, at least.
40* ''VisualNovel/DaCapo'' features another almost-incest relationship between a boy and his [[NotBloodSiblings foster sister]].
41* ''Manga/DearBrother'' has [[spoiler: Rei Asaka's obsession with her older half-sister Fukiko Ichinomiya, and Fukiko's abusive behavior towards her]] as one of the driving forces of the plot.
42* In ''Manga/DGrayMan'', Komui's attitude toward his sister Lenalee straddles the line between BigBrotherInstinct and this trope; even the other characters find it a little creepy and frequently remark on his obsession.
43* WordOfGod is that Hikari from ''Anime/DigimonAdventure'' has feelings for her brother Taichi. It was presented in a subtle way that [[ParentalBonus kids weren't supposed to get but older fans probably would]]. Episode 21 was written similar to a love story.
44* ''Anime/DigimonAdventure02'': Mummymon has a one-sided crush on his partner-in-crime Archnemon. It is much later revealed that they were created from the same DNA, Oikawa's. This makes them siblings due to both of them sharing the same father. Their identity crisis of them being neither fully Digimon nor fully human doesn't help either.
45* ''Literature/{{Dokkoida}}'' [[PlayingWithATrope deliberately plays with this trope]] since Suzuo's [[MissionControl Mission Controller]], Tampopo, [[SecretIdentity disguises]] herself as his sister, Kosuzu, to blend into Earth society. The eighth episode in particular parodies this trope to the point of full-fledged {{Deconstruction}}: seeing a soap opera using this trope makes all of the PrettyFreeloaders in the apartment the characters live in worry about Kosuzu/Tampopo's relationship with Suzuo -- including Kosuzu. Suzuo doesn't notice a thing, and manages to [[ResetButton restore the status quo]] with a "dunno what's going on, but..." speech at the end.
46* In ''Manga/DomesticGirlfriend'' the main character loses his virginity to his step-sister and is in love with his other step-sister. These all happened BEFORE they became a family.
47* Being focused on otaku it's no surprise this trope gets brought up and discussed in ''[[Manga/ShinozakisanKiWoOtaShikaNi Don't Become an Otaku, Shinozaki-san!]]'' Makoto's otaku buddies are jealous the he has a real life little sister, but Makoto himself feels no attraction to her (least of all because she tends to treat him badly), nor to any of his sister's friends specifically ''because'' he's known many of them since they were very young. Akina has no interest either, viewing Makoto as a gross otaku even as she becomes one herself, and because she's more a lesbian anyway.
48* ''Manga/DontMeddleWithMyDaughter'': In chapter 9, Artemis challenges her older sister, Athena, to a duel to decide whether she and her daughter would have to [[spoiler: return to their home planet with her]]. The match is even, until Athena catches Artemis off-guard by suddenly tongue kissing her. Then she capitalizes on Artemis' shame and confusion by fingering her, and finishes her off by 69ing with her in midair. Artemis [[BeatThemAtTheirOwnGame tries to retaliate]], but she climaxes first. She concedes the match, but still [[WhatTheHellHero calls Athena out]] for using such [[DefeatByModesty underhanded tactics.]]
49* ''Literature/{{Durarara}}'': Namie is quite blatant to admit that her love for her younger brother Seiji is not familial. Even Izaya finds it a bit disgraceful.
50* In Yuki Suegutsu's infamous ''Manga/EdenNoHana'' manga, the protegonist, Midori, was put in foster care and then was raped by her adoptive brother.
51* All but expressly stated in ''Edens Bowy'' between tragic siblings Wietoo and Hairra. Initially averted when Hairra actually tried to pair her older brother Wietoo together with her best friend Fennis in an effort to repress her own incestuous feelings, but it failed anyway due to irreconcilable differences of ideology between Wietoo and Fennis, who also noticed Hairra's disturbingly excessive attachment to her sibling. Eventually the siblings get together when Hairra decides to acknowledge her feelings upon realizing that no one would love her brother as much as she did (which led to a falling-out with Fennis) and Wietoo also realizing that only Hairra would ever accept all of him, including his controversial views on the creation of sentient A.I. Hairra is soon killed tragically sometime afterwards when Wietoo's first android goes berzerk, and she is reborn as his personal gynoid weapon/bodyguard. Later, when they are both killed at the series end, they are reborn together as sentient programs together roaming forever their world's version of the internet.
52* In the Manga for ''Manga/ElfenLied'', [[spoiler: this is the entire reason Lucy/Nyuu/Kaede's half-brother was born. Kakuzawa wants him to mate with Lucy/Nyuu/Kaede and procreate more Silphelits.]]
53* ''Literature/EromangaSensei'' from the same writer/illustrator team as ''Literature/{{Oreimo}}'' makes it clear that step siblings Masamune and Sagiri are attracted to each other almost from the outset. Masamune straight up declares his love in episode 3, though neither pursues it in part due to hang ups from their loss of family members -- each is an orphan when the series begins, and he hadn't seen her in the 2 years since their parents died and she became a hikkikomori.
54* This is a plot point in ''Manga/EternalAlice''. The main characters are a boy named Aruto, his love interest Arisu, and his little sister with a very obvious crush on him, Kiraha. It is revealed later that [[spoiler:Arisu isn't real, and Aruto made her up because he couldn't deal with his incestual feelings for Kiraha.]]
55* Ryo from ''VisualNovel/FinalApproach'' is quite obsessive over his little sister Akane and she's generally reciprocative. It's generally played for laughs as nothing more than an amusingly intense sister/brother complex, but it is worth noting that Akane insisted on taking part in the contest with [[UnwantedHarem the other girls]] to see who got to play the role of Ryo's bride.
56** When Akane is sick Ryo rushes home to take care of her. It's at this point the other girls realize they're not competing with each other but with Akane.
57* Averted in ''Manga/FistOfTheNorthStar'' when Juuza discovers his childhood sweetheart Yuria is actually his half-sister. They drift apart soon after, having never consummated their relationship.
58* Averted in the final arc of ''Manga/FoodWars''. Asahi Saiba shows up out of nowhere and [[AndNowYouMustMarryMe tries to force Erina Nakiri to marry him]], much to her displeasure (and Soma's, for that matter). After Soma defeats Asahi in the BLUE, it's revealed that he's the son of Azami (Erina's father) from a one-night stand he had with a random woman when he was a student.
59* While it's not ''quite'' this, ''Manga/FrankenFran'' has a man named Kusinoki have sex with some sort of octopus hybrid that greatly resembles his deceased sister Azusa, and the dialogue suggests he had wanted to do this to the real Azusa too. [[spoiler:[[KarmicDeath His body is used as nourishment for the octopus hybrid's numerous offspring.]]]]
60* In ''Manga/{{Freezing}}'', Satellizer was abused by her half brother. He molested her as well, though their blood relation was in fact the only thing that kept him from outright raping her, what he did was enough to emotionally scar Satellizer to the point that she could no longer stand being touched by ''anybody''.
61** And, for some irony, the one guy who's the exception, the one guy who can touch her because he treats her with dignity and compassion? He was attracted to her initially due to her resemblance to his big sister, and [[spoiler: and given they share genetic implants tied to said sister that give Satellizer a genetic resemblance, they are an indirect example of this trope as well, except Kazuya Aoi (the guy) is a far, far better choice for Satellizer by far.]]
62* ''[[PlayingWithATrope Somethinged]]'' in ''Anime/FreshPrettyCure''. Miki's brother Kazuki lives with her father (her parents are separated, presumably divorced) and uses his name. She hasn't told her best friends they they're related, and in the first episode she refers to their upcoming outing as a date. The second episode focuses on one of their dates; it also features TheReveal that they're related, but you'd be forgiven for missing this detail. Eventually it's revealed that, when her friends assumed Kazuki was Miki's boyfriend, Miki went out of her way not to correct them because she thought it was cute.
63** Considering the [[LesYay yuri subtext]] in the ''Anime/PrettyCure'' franchise in general, we have the only Precure-sisters Love and Setsuna from the same series. They are step-sisters, though.
64* ''Anime/FromUpOnPoppyHill'' uses it as a ZigZaggedTrope when Umi and Shun fall for each other, find out they're related, resolve to continue the relationship anyway, then find out they're definitely ''not'' related. As far as they knew, they definitely fell into the trope for the few hours between deciding to continue and finding out the truth, though nothing physical happens during that time.
65* Renhou and his older sister Miiru in ''Manga/FushigiYuugi''. [[spoiler: In fact, their incestuous relationship got them killed by their people, and they go to the BigBad Tenkou in hopes he'll help them stay together.]]
66* In ''Anime/{{Gankutsuou}}'' Andrea actually tries to marry and rape his half-sister Eugenie. Though only to deliberately humiliate her and her family.
67* Silvia in ''Anime/GenesisOfAquarion'' has a pretty obvious attraction to her older brother Sirius, becoming incredibly jealous of even the slightest hint of romantic interest in other women. It seems her insistent belief that he is the reincarnated lover of the woman she is the reincarnation of has a lot to do with this. [[spoiler: In reality, reincarnation is largely to blame, but not in the way it first seems: they are actually both reincarnated from the same person, each holding a fragment of Celiane's soul. The longing of the two halves to be united again causes Silvia's apparent crush on her brother; he doesn't feel the same way because he got stuck with the negative aspects of Celiane's soul and memories, and so sealed them away at a young age.]]
68* In ''Manga/{{Georgie}}'', the PluckyGirl Georgie is romantically pursued by her two adoptive brothers, [[RedOniBlueOni Abel and Arthur]]. Since the three were raised together and Georgie herself didn't know she was adopted for many years... well, yeah. In the anime, she doesn't end up with either of them but they leave together as a family. In the manga, however, it ends ''very'' differently. Georgie does fall for oldest sibling Abel. They even have the [[SexyDiscretionShot shoujo equivalent to a sex scene]]. By the end of the manga, Abel has been killed and Georgie has his child, and decides to return to Australia, [[PairTheSpares where Arthur is waiting for her]], having returned several years earlier.
69%%* ''Manga/{{Genshiken}}'' pokes fun at this, with Sasahara, who actually ''has'' a sister, delivering the quote from the main page.
70* In ''Anime/GhostHound'', the main character, Tarou, has a slight obsession with his sister which leads to him accusing the girl he likes of being his sister reincarnated as an explanation for why he thinks about her all the time.
71* In ''Anime/{{Godannar}}'', English pilot Knight Valentine will flirt with any female in sight, much to the consternation of Ellis, his sister and partner, who thinks he doesn't notice her. But if [[spoiler:the way he looks at her swimsuit-clad form and the fact that he sees Ellis' face when an amnesiac Mila is hitting him with CharmPerson are any indication, he ''does'' notice her in that way.]]
72* ''Manga/HappyWorld'': The explanation Takeshi uses to explain Elle's connection to him is that she's his step-sister. Cut to Takeshi exclaiming loudly to her in a classroom "We should make a baby." And they actually go halfway through the act before realising that they're not emotionally ready for it.
73** As it turns out, their relationship to eachother [[spoiler: ''is'' technically step-siblings... probably. Takeshi's mother is Elle's mother, but Elle doesn't have a father, so it's a bit complicated.]]
74** Their child, Prayer, is eventually born... but not through normal means. Takeshi and Elle [[spoiler:fuse their bodies, and Prayer is born when they split apart again.]]
75* Two examples of this, running in both directions, are present in ''Manga/HatsukoiLimited''. Ayumi's brother, Yuuji, is a definite siscon, and Koyoi Bessho is defined by her brother complex. Of course, they are but one small part of the LoveDodecahedron that permeates the series.
76* This issue is addressed in episode 9 of the second season of ''Anime/HellGirl''. The incestual love in this case is one-sided, and the offending sibling is, of course, sent to hell. The episode ends on a lighter note; the aforementioned sibling is not tortured on the ferry (unlike sinners who have done worse), and there is the knowledge that the brother and sister will eventually be reunited as per the terms of the curse.
77* ''Webcomic/HetaliaAxisPowers'' has both the traditional brother/sister version ''and'' a HoYay one. On a hand, the {{Yandere}} Belarus would love to marry her older brother Russia (also a {{Yandere}}), which freaks him out no end. On the other, the AnnoyingYoungerSibling South Korea isn't much more successful in getting his older brother China (and sometimes his ''other'' older brother, Japan), to acknowledge his more-than-brotherly affection. Both cases are played for laughs.
78** Switzerland and Liechtenstein, while NotBloodSiblings are shown in-canon to be ''very'' close, leading to some ShipTease. It probably doesn't help that they also act like a young married couple at times rather than as brother and sister.
79* Somewhat referenced to in ''VisualNovel/HigurashiWhenTheyCry''. Mion Sonozaki mentioned in the sound novels that she'd marry her twin sister Shion if she was a boy, ''not'' mentioning if they weren't related. [[IncestSubtext The official art including the sisters]] [[FanService does NOT help.]]
80* ''Hime-Sama Love Life'': An unpopular brother and his shy sister decide to have their first times with each other.
81* In ''Manga/HisAndHerCircumstances'', Kazuma and Tsubasa simutanously get crushes on their other as their parents get married. Cue SickAndWrong from parents, {{Wangst}} from Kazuma which make him run away from home, which makes poor Tsubasa down the mental road before it gets better.
82* Shinogu and Hatsumi from ''Manga/HotGimmick'' are NotBloodSiblings. Shinogu is in love with Hatsumi but, in an effort to not burden her with his feelings, he tries to put distance between them. Hatsumi actually offers herself sexually to Shinogu at one point but he turns her down. And in the Hot Gimmick S novel, she actually leaves her [[DomesticAbuse abusive boyfriend]] Ryoki for Shinogu.
83* In the light novel which ''Manga/HybridXHeartMagiasAcademyAtaraxia'' is based on, Kizuna (age 17) has his first [[DeusSexMachina Climax Hybrid]] session with his biological 27-year-old sister, Reiri, in volume 5 because there were no other options. However, a flashback to their childhood shows Reiri had an unnatural interest in her brother, even then and often bathed with him. They have additional sessions in volumes 8 and 10, by which time, Reiri fully accepts that she's in love with Kizuna. By volume 13, [[spoiler:they have unprotected sex almost every day, ''[[ExtremeLibido usually for hours]]'' at a time. It eventually results in Reiri being the first to become pregnant with his child]].
84* For the first volume of ''Manga/{{Ichiroh}}'', Nanako's brother had it bad for her and even followed her while she was off studying for exams. Nanako, of course, did not return these feelings. Eventually, he realized his sister was maturing while he was not and set off to improve himself to be worthy of her. We never see him again except in passing and omake strips, presumably because incest jokes in a light-hearts 4-koma strip would be kinda out of place.
85* ''Anime/InfiniteRyvius'': [[spoiler:We eventually discover that Ikumi had fallen in (that kind of) love with his dead older sister]].
86* ''Literature/TheIrregularAtMagicHighSchool'' has Miyuki Shiba, who is ''very'' attached to her older brother Tatsuya and has [[spoiler:deliberately]] geared a good part of her life around him. On the other hand, Tatsuya certainly cares for Miyuki but he's got his own and huge issues and can barely feel actual emotions as a result. [[spoiler:It turns out that Tatsuya's only ''truly'' remaining emotion is his brotherly love for Miyuki, but later, so as the body of his sister genetically "improved" is enough to nullify any effects of incest between siblings, their aunt announces Tatsuya her son and adopts him, so that they could marry as cousins. So later in the story, [[ArrangedMarriage they're engaged to marry]].]]
87* ''Manga/TheKindaichiCaseFiles'' manages to have two examples within its first serialization.
88** In '''Lake Hiren Murder Case''', a man masquerading as a serial killer is on a RoaringRampageOfRevenge for his dead lover... it's revealed after he is DrivenToSuicide that said lover was his sister. (And the lake where the murders take place is rumored to have been the suicide spot of another pair of sibling lovers.)
89** An accidental yet very twisted example in '''Black Butterfly of Death Murder Case'''. The culprit infiltrate the Madarame Family by [[spoiler:planning on marrying one of the daughters (during the setting they were already engaged with each other)]] while actually the culprit was there in order to kill the entire Madarame Family because the culprit was an illegitimate child of [[spoiler: Midori (the family's mother) and Minoru Suga whose research about luminous butterfly was stolen by Shimon Madarame]]. However, the sisters were not [[spoiler: Shimon Madarame]]'s biological daughters; instead [[spoiler: they were born from artificial insemination using Minoru's sperm]] making the culrpit and the three sisters biological siblings. What's worse is that the sister is shown to like the culprit. Yikes yikes '''YIKES'''.
90* ''Kiss x Sis'' is about the ongoing incestuous affair between Keita and his older twin stepsisters, [[TomboyAndGirlyGirl Ako and Riko]], who take turns waking up him up each morning by making out with him beneath the covers. Their trysts [[HotterAndSexier become increasingly risque]] as the series (and their relationship) progresses, to where Keita has even brought [[{{tomboy}} Riko]] to orgasm by [[spoiler: performing oral sex on her]]. Despite all this, they have yet to actually engage in intercourse, which remains in question as the manga is still ongoing.
91* ''Manga/KoiKaze'' is a case of this trope being [[DeconstructedTrope deconstructed]] and PlayedForDrama with two blood siblings who haven't seen each other since the brother was 18 and the sister was only 5 when their parents divorced and their mom took sole custody of the sister, and as a result, the UsefulNotes/WestermarckEffect didn't take hold. The series has a more harrowing portrayal and doesn't gloss over just [[InconvenientAttraction how painful and frustrating]] such a situation would be for both parties involved.
92* Iina from ''Manga/KokoroLibrary'' appears to have quite a thing for her sister Kokoro. She tries to steal kisses from her and gets quite upset when Kokoro decides to spend time in the bath with her ''other'' sister, Aruto, instead of her. Kokoro clearly loves Iina a lot too, but in a ''sisterly'' fashion.
93* In the spinoff of ''Manga/{{Genshiken}}'', ''Anime/KujibikiUnbalance'' has the main character's sister pursuing him endlessly, even going so far as to [[spoiler: try perfume that is actually a love potion to seduce him. Instead she just succeeds in getting MC's best friend to kiss MC much to his sister's despair and anger]].
94* Even though very rarely used in a YuriGenre anime, the first episode of ''Anime/{{Kuttsukiboshi}}'' has [[spoiler:main character Aya cheating on her girlfriend, by having sex with her own terminally sick biological brother in a surprisingly CruelTwistEnding.]]
95* The secret of the [[BigScrewedUpFamily Kuhouin family]] in ''Literature/{{Kurenai}}'' is that [[spoiler:each girl born into the family is imprisoned in the "Inner Sanctuary" for their entire lives, for the sole purpose of bearing the children of their brothers to continue the lineage. Their very existence is kept hidden from the public, and the male members of the family marry women from outside the family just to keep up the masquerade]].
96* [[BigBad Maestro Delphine]] from ''Anime/LastExile'' has a fascination with her brother [[TheRival Dio]]. Dio fears her to death, with good reason as she is completely AxCrazy.
97* ''Love 2 Quad'': The hero gains power via ecchi activities, one of which is deflowering his younger sister.
98* [[spoiler: Seimei is a LoveMakesYouCrazy version]] in ''Manga/{{Loveless}}'' towards Ritsuka. [[spoiler: To Seimei, him and Ritsuka are the only two people in the world. The fact that the two are full-blooded brothers is probably the least [[{{Squick}} squicky]] thing about him, though.]]
99* ''Manga/{{Magikano}}'' plays with the trope: The main character's oldest sister has a downright obsessive love for him (one that is definitely not simply sisterly) even to the point where she claims she will marry him and becomes ultra possessive whenever any other girl shows any interest. However he doesn't seem to have any interest in her. The arrival of the protagonist's MagicalGirlfriend makes her intensely jealous and always vying for his attention. Despite the show's fanservicey roots, it's never portrayed as actually romantic or sexual though. The manga on the other hand...
100* In ''Mahou Shounen Majorian'', Mari falls in love with the magical girl form of her brother, Masaru--which makes it kind of hard to classify.
101* In ''Manga/MarmaladeBoy'', Yuu and Miki become [[NotBloodSiblings step-siblings]] when their parents marry (with both sets of parents splitting up and marrying each other), and then [[FlirtyStepsiblings find themselves growing attracted to each other.]] [[spoiler:Later in the series, they find evidence that they may be actually blood siblings; before they learn that this was all a big misunderstanding (said evidence wasn't referring to Yuu but to his mom's still-born child, who would've been his older sibling), they decide that they would marry regardless with the understanding that they will never have children.]]
102** Interestingly, the Mangaka (according to [[WordOfGod her notes on the series]]) originally planned for them to discover that they ''were'' blood siblings and split up for good, eventually hitching up with their unrequited love interests in a DistantFinale. Her producer convinced her that it wouldn't go down well with the fans...
103* In ''Literature/MazeMegaburstSpace'', not only is the central couple a pair of blood siblings, and not only is the central plot their successful and unrepentant plot to be together, but it goes even beyond that...
104* It's mentioned in ''Manga/MissKobayashisDragonMaid'' that Lucoa (who is a {{Gender Flip}}ped [[Myth/AztecMythology Quetzalcoatl]]) [[ShownTheirWork once slept with her sister after getting drunk]]. She ended up losing her godhood as a result, and [[OldShame really doesn't like it when people bring it up]].
105* ''Literature/{{MM}}'' main character Taro is actively pursued by his older sister ''and'' [[ParentalIncest mother]]. When he gets amnesia for an episode, both try to pretend being his pregnant lover.
106* This is a catalyst for one of the storylines in ''Anime/{{Mononoke}}''.
107* Yui from ''Literature/MuvLuvAlternativeTotalEclipse'' is in love with Yuuya even after eventually learning he's really her half-brother.
108* [[Franchise/{{Nasuverse}} Nasu Kinoko]] ''loves'' this trope:
109** In ''VisualNovel/{{Tsukihime}}'', Shiki's sister Akiha has a very possessive crush on him. Parodied in numerous doujinshis and official {{Omake}}s, including one where Ciel points out the hopelessness of the crush. Shiki's feelings are purely platonic. And a large part ''sheer terror''--Akiha is '''[[{{Tsundere}} tsun]]'''[[{{Tsundere}} dere]] at best and {{yandere}} at worst. Of course, [[spoiler:they ''are'' NotBloodSiblings, and]] Akiha does have her own path in the VisualNovel.
110*** Though they never did anything incestuous, [[spoiler: SHIKI]] hints many times that he has romantic interest in his sister [[spoiler: Akiha. Too bad all she thinks about is her other "brother"]].
111** On that note, Azaka from ''Literature/TheGardenOfSinners'', who can be considered prototype of Akiha above, also loves her brother deeply, to point where it gets in the way of her becoming friends with the series heroine Ryogi Shiki. Her love may not be the same in nature as Akiha's but since she and her brother were used as a strong basis for Akiha and Shiki, it is likely that the "love for my brother" was carried over as well.
112** Illya of the ''Fate'' series invokes this ''twice'', with varying degrees of reasoning:
113*** In ''VisualNovel/FateStayNight'', Illya is Shirou's adoptive big sister (though looking like a little sister), and she is as calculating and sadistic as Akiha above. In some Bad Ends, she does very bad things to Shirou, and in one cut-out scene would've gone so far as to rape him -- but after her resentment towards him and their father is revealed to be a mistake, she severely mellows out... though the attraction remains and she's not shy about it. There would've been a route that explored this, but it (along with the rape scene) was cut due to a development timecrunch.
114*** In ''Manga/FateKaleidLinerPrismaIllya'', Illya is instead ''actually'' Shirou's little sister by adoption. Though this Illya is much better adjusted than her mainline counterpart, the BigBrotherAttraction is still there and EveryoneCanSeeIt, much to her horror. It eventually gets pushed out of the spotlight when Illya has to deal with ''receiving'' incest of more fantastic sorts, namely Chloe, a magical clone-turned-sister/cousin of Illya who needs to at least be kissed to stay alive, and [[spoiler:Miyu, who turns out to be an AlternateUniverse counterpart to Illya and has ended up in compromising situations with her more than once]].
115* ''Manga/NinjaNonsense'' has Sasuke imagining that he's the object of an UnwantedHarem. He disturbingly imagines that Shinobu is his sister.
116* Happens in ''Manga/NozomuNozomi'' when [[spoiler:Nozomu's sister [[GenderBender turns into a boy]]. She gets a RagingStiffie that won't go away. Nozomu tells her to do the obvious but she's too scared so he "helps" her, just this one time]].
117* In ''[[Literature/ArabianNights One Thousand and One Nights]]'', Dunya is infatuated with her older brother Sehara. She actually demands that he kiss at one point (which he does).
118* ''Oniichan Control'' has the brother who fled from his younger sister because he's developed inappropriate feelings for her. Unfortunately, his younger sister is not only fully aware of these feelings, but returns them in a big way. ''And'' she's a first-rate [[ManipulativeBastard manipulator]] beneath her cutesy facade, managing to track him down, get admission to the same college he goes to, and worm her way into becoming his roommate. This goes so far that she's willing to [[MurderTheHypotenuse stab a girl she thought was interested]] [[BrotherSisterIncest in her brother]]. The entire series is about the brother trying to ''avoid'' this trope while the sister tries to ''invoke'' it.
119%%* It's the whole point of, and PlayedForLaughs in ''Literature/OniAi''.
120* In ''Manga/OokuTheInnerChambers'', it's revealed that Akimoto and his sister Kinu slept together, resulting in a daughter. When Kinu asked if he entered the Ooku because he was disgusted by what they did, his response was that if he hadn't, he'd be forced to marry, and he didn't want to father a child with anyone but her. Emonnosuke is ''quite'' amused when he finds this out about Akimoto, and points out that even Japan's origins center around this trope. It's probably pretty telling that he actually gets one of the ''happier'' endings: [[spoiler: after Shogun Tsunayoshi dies, he retires and returns home, meeting his daughter/niece for the first time, and is said to have lived the rest of his life out in peace with his family.]]
121* ''Literature/{{Oreimo}}'': Whether or not siblings Kirino and Kyosuke do or don't have a sexual attraction/hidden desire to each other is the ElephantInTheLivingRoom of the series. The anime adaptation leaves it hanging; the last chapter confirms it.
122* ''Manga/OukeNoMonshou'' has the {{Ancient Egypt}}ian princess Isis who is in love with her brother, Pharaoh Memphis. Her jealousy towards any woman remotely interested in him [[spoiler:extends beyond the grave]], and it actually kicks off the plot [[spoiler: since Carol, {{The Hero}}ine, fell victim to one of Isis's curses and then was thrown into the Egypt of the past...]]
123* ''Anime/PantyAndStockingWithGarterbelt''[='=]s Scanty and Kneesocks engage in plenty of sex when their father is not looking.
124* ''Anime/{{Penguindrum}}'''s first episode has Kanba Takakura kissing his younger sister Himari after she's resurrected and while she's sleeping. It hasn't been referred to later, though. [[TemptingFate Let's give it time.]]
125** It's given a rather philosophical twist in this series; Kanba's InternalMonologue in the scene revolves around [[ScrewDestiny defying fate]] and the animalistic [[ArcWords survival strategy]] to determine one's free will. Basically, doing something that your very biology abhors is used as a sign for freedom of choice.
126** In the Penguindrum novel, the scene where a posssessed Himari pulls something out of Kanba's chest ([[spoiler: his life energy]]) is a little more suggestive than in the anime version. Not to mention, in the anime the kiss scene went into fade to black when Kanba's lips were milimeters away from Himari's. In the novel? It's described quite in detail.
127** Taken further in Episode 12, where [[spoiler: before dying again (temporarily), the possessed Himari shares a last goodbye with Kanba. When they're the only ones in her Dream World. [[http://imageftw.com/uploads/20110929/1317318196481.jpg While she's pretty much naked except for her boots and the Penguin Hat, and Kanba's chest is exposed due to self-inflicted Clothing Damage]]. [[DoesThisRemindYouOfAnything A sexual encounter wouldn't have been out of place]].]] Though there ''may'' be a twist here, [[spoiler: with the sex-charged interaction itself being more in between Kanba and the ''possessor'', the Princess of the Crystal, rather than between him and Himari. [[MindScrew It's... hard to tell]].]] It happens again in episode 17, when [[spoiler: Himari/the Princess [[http://i42.tinypic.com/2wp8c51.jpg strips again]], [[CooldownHug cradles]] [[http://i39.tinypic.com/2em0gih.jpg a depressed Kanba to her/Himari's bare bosom]], and pretty much [[http://i40.tinypic.com/243mely.jpg throws herself into his arms]]. Again, not clear who is who here, but still. . .]]
128** Aaaand then... [[spoiler: it's ''subverted''! Himari and Kanba are NotBloodSiblings. Episode 19 explains that Himari was adopted into the Takakura family after she was rescued from a plance named the Child Broiler... and her savior was her and Kanba's brother, Shouma. Kanba himself is adopted too; he and Shouma relied on each other to survive, but he only felt truly as a Takakura when Himari welcomed him into the family.]]
129** Then finally reintroduced in the form of {{Twincest}} when [[spoiler: Masako, Kanba's stalker and apparently jealous ex, turns out to be his biological twin sister, and Masako and Kanba were both well-aware of their relationship the entire time. Having physically assaulted and mind-wiped the rest of Kanba's ex girlfriends since she didn't see them as good enough for him, Masako ultimately turns her sights on Himari, whom she sees as both familial and romantic competition, but later sets it aside when Kaba pulls a FaceHeelTurn.]]
130* ''Anime/PleaseTwins'' has what may or may not be an incestuous LoveTriangle, between a boy and two girls who both claim to be his twin sister. To add to the blurring of the line, the girl who turns out to actually be his twin is different between the anime and the novelization. Interestingly, though, the setup of the plot clearly steers ''away'' from this: whoever turns out to be the twin must step out.
131** That said, both girls kiss Maiku before it's over, so be ready for some {{Squick}}. The possibility of being his sister keeps them from getting really aggressive, but that's ''all'' it does; their ambitions are not deterred.
132** Meanwhile, perhaps to provide contrast, there's a minor character (Matagu) who's ''blatantly'' attracted to his little sister Haruko. And Haruko doesn't quite reciprocate, but at least clearly knows and doesn't mind. After describing his use of the HandsOnApproach, we get this:
133---->'''Haruko's friends, in unison''': Your brother's a pervert!
134---->'''Haruko, cheerfully''': He ''is!''
135** The attraction in this series isn't all that unrealistic. The UsefulNotes/WestermarckEffect isn't in play, since the twins were separated at a very young age (maybe 2 at the latest), and the general principle of genetic attraction makes it unsurprising that the sister would have some feelings upon seeing someone who may (not) be her brother. That the other is a childhood friend makes it all the more probable, since they would have subconscious affection for one another at the outset. There is some kissing and some hand-holding/hugging between the would-be sister and the brother. Otherwise, this is an AvertedTrope, since, as stated, the twin is not allowed to continue the relationship (which is part of the drama: which girl has to give up her romantic affections, and which girl gets to be by his side albeit as a sister, not a lover).
136** Maiku does have a hard time not having sex with either of them in the BeachEpisode (complete with imagining them naked, though admittedly chibified) and has to resort to leaving the room to contain his libido. Knowing one is definitely his sister doesn't stop him from wanting them BOTH, although in fairness he doesn't know which one, nor does he act on it.
137** Ultimately, the characters are fully aware that such a relationship is ultimately not acceptable, to the point that both girls hope that they aren't the sister, possibly allowing a kindling of a relationship. Regardless of which one turns out to be the sister based on medium, the story presents this as a tragic thing, and said girl does stop the relationship in awareness of the gravity of the situation.
138* In ''Anime/QueensBlade'', Elina [[BigSisterAttraction lusts after]] [[LustObject both her older sisters]]:
139** She wants to with sleep with Leina and pouted when she wasn't allowed to spend the night in her room, in the first episode. Leina is more than aware of Elina's blatant incestuous crush on her, which is part of the reason she ran away from home and doesn't intend to return. Though she still [[SkinshipGrope indulges Elina on occasion.]] Such as Episode 9, where she bathed together with her, then spent the night spooning with her in bed, while both were in the nude.
140** It's also implied in the main series that Claudette is in love with Elina, but her feelings are unrequited due to Elina being in love with Leina.
141** The ''"Hide & Seek"'' manga continuity took the subtext and [[LoveTriangle ran with it]]. While Elina's feelings for Leina remain paramount, the manga features a romantic subplot between her and Claudeete and even has Leina say [[ShipperOnDeck she supports her sisters being together]] (Chapter 28).
142* In ''Anime/RevolutionaryGirlUtena'', not only does Touga's sister Nanami harbor an intense crush on her older brother, but she also has a [[ClingyJealousGirl near-insane jealousy]] of anyone or anything that might attract more of his attention and love than she does. There is also the strange love-hate relationship between Kozue Kaoru and her twin brother Miki, best displayed in the bizarre bathtub-and-straight-razor scene from the ''Utena'' movie. Most important to the plot, though, is [[spoiler: the semi-consensual relationship between Anthy and her manipulative and domineering brother Akio]]. In the first example, a few of the last episodes of the series {{deconstruct}}s Nanami's infatuation with her brother by introducing the possibility that they are not siblings [[spoiler:and then reveals that they are siblings; Touga is just being manipulative]] and explores [[ShooOutTheClowns Nanami's emotional confusion resulting from that distancing]].
143* In ''Literature/SaikyouJuzokuTensei'', the story stars a Japanese high-schooler who [[ReincarnateInAnotherWorld reincarnates into a world of swords and sorcery after his death]], which he comes to love because there is magic, and he was reborn into a tribe of strong magicians called the Marren Clan. But when he, Abel, reached the age of sixteen, he was surprised by his father, telling him to [[ArrangedMarriage prepare for his marriage]] ..'''to his [[BigBrotherAttraction little sister Gizel]]!!''' Turns out the Marren clan has a tradition, of brothers and sisters marrying after the brother's coming of age, if they're less than 5 years apart. Their reason for this is their belief that a pure bloodline leads to stronger family bonds and offspring with stronger magic powers. He's also a product of incest, like the rest of the clan. This leads the protagonist to [[ScrewThisImOuttaHere escape the village]] and [[DesperatelyLookingForAPurposeInLife find his calling in life]]. Later, [[IWillFindYou his sister manipulates the village into forming a search party]] to find and force him back into the village and their marriage.
144* Ali and En in the anime-only "Makaiju" ("Doom Tree") arc of ''Anime/SailorMoon''. This is explainable to an extent, however: not only are they [[LastOfHisKind the only surviving members of their species]], but they are "children" of a tree, which used what remained of its powers to "grow" them after the rest of the species was destroyed in warfare; thus making them "siblings" in roughly the same sense as Adam and Eve.
145** Lampshaded once by Makoto, when "Natsumi"/En bitches "Seijuurou"/Ail out for letting Mako offer him a part of her lunch. She's squicked and says something by the lines of "Wow... these siblings! So weird!"
146** [=DiC=], perhaps as bowdlerization, had Crimson Rubeus refer to his four main female minions as sisters. He may have been referring to their name as a group, the Phantom Sisters, but it's suspicious when you realize most of them are attracted to him.
147* ''AudioPlay/SaintBeast'': Definitely played with in Kira and Maya, particularly in the official art.
148* In the anime of ''Manga/SamuraiDeeperKyo'' , ''BigBad'' Nobunaga Oda and his right-hand henchwench Shatora are an incestuous brother-sister pair who desire who turn the "boring world" of Sengoku-Era Japan into a literal "hell of battles" so they can openly be together in the chaotic new world. They reveal that their ''StartOfDarkness'' was because society tried to force them apart by making their relationship taboo in the first place.
149* It is heavily implied in the ''Manga/ShadowStar'' anime that the bully Aki Honda and her older brother Yasuhito have a ... rather intense relationship. It's downright spelled out in the manga. [[spoiler: So much that Aki is once shown straddling Yasuhito while practically naked. Then, Oni shows up, kills Yasuhito by smashing his head on the wall, and rapes and kills Aki as revenge for what they did to his master, Hiroko.]] Said scene is censored in many countries in the world (even the Japanese version got censored retroactively) and spelled the end of the series' publication in many others.
150* Kanan is Hakkai's older sister in the ''Manga/{{Saiyuki}}'' manga, although this was edited out of the anime version, and, as they had been SeparatedAtBirth or very close to it, they weren't originally aware of it. A tie-in novel also suggested that they might be {{Twin|cest}}s as well, which was approved by WordOfGod.
151* 15-year-old Sakurako Saiki from ''Manga/SakuraGari'' is a scary {{Yandere}} who is incredibly possessive of her older brother, the main character Souma, [[spoiler: and has had sex with him more than once.]]. For further subversion, [[spoiler: Sakurako is actually a ''male'', Youya, who calls himself like that after the horribly fucked-up death of his mother... whose name was Sakurako.]]. And even ''more'' subverted: [[spoiler: They're NotBloodSiblings. Souma is the son of the Saiki leader and his dead lost love, Abigail; Youya/Sakurako is the child of the original Sakurako ''and'' the retainer Katou. When Youya/Sakurako commits suicide, Katou reveals this and stabs Souma -- apparently, to death.]]
152* Deconstructed to the point of FanDisservice in ''Literature/SasamisanAtGanbaranai'': In order to keep the power of Amaterasu in their bloodline, siblings in Sasami's family are forced to marry. Due in part to what is apparently a lobotomy, the sibling which does not possess Amaterasu's power is conditioned to care for and unconditionally love the one that does.
153* Tiriel and Sorath in ''Literature/ShakuganNoShana'' have an incestuous relationship, though in this case it's portrayed in a creepy light. [[DemonicInvaders What they]] [[EnergyBeings are]] muddles the water, but only somewhat. Shana seems to take more offense at the French kissing than anything else.
154* An example also occurs in Yuu Watase's pre-''Manga/FushigiYuugi'' manga, ''Manga/ShinshunkiMimanOkotowari''. Asuka Higuchi falls for her half-brother Manato Sudou early on in the story, and he eventually comes to reciprocate. [[spoiler:And then they learn that they aren't blood-related after all--in a very mind-twisting way.]]
155* ''Shoujo Sect: Innocent Lovers'': In the first episode, Chizuru goes to Naitou for advice on how to confess her feelings for the girl she was secretly in love with. Since Naitou didn't feel like being bothered, she tells her to just say so and get it over with. Adding that she didn't want to hear anymore about it until Chizuru had finally done it, and demanded she provide proof. So [[BigSisterAttraction she confesses her love to Shigure that night.]] Just when it seemed that her feelings were unrequited, Shigure reveals she'd secretly been in love with her too and makes love to her. The next day at school, Chizuru delivers on her promise by showing Naitou a photo of them in bed together, which she had taken with her cellphone camera. When Naitou sees the other girl had been Chizuru's older sister, [[MyGodWhatHaveIDone she regretted having said anything]] since, had she known, she would've advised against it.
156* [[spoiler:Hoshina Utau]] from ''Manga/ShugoChara'' ''loves'' the resident PrettyBoy catboy of the series, Tsukiyomi Ikuto. The thing is, [[spoiler:her real family name is Tsukiyomi and she ''is'' Ikuto's sister]], which does explain why Ikuto is so cold towards her advances -- in fact, [[spoiler:he's downright disgusted when she kisses him]]
157* Alti in ''Anime/{{Simoun}}'' is obsessed with her sister Kaimu, and tries to prod her into choosing to be female so that Alti can become male and "protect" her.
158** They also have sex once during the course of the show (long story) and in the DistantFinale they are living together [[spoiler: even though they both chose to remain female]].
159* ''Manga/SisconAniToBroconImoutoGaShoujikiNiNattara'' has Ritsu and Uta Fukami. Originally they are merely close siblings, but after Uta is annoyed that Ritsu plays with her less due to his friends keeping him at school more often, including one girl that shows interest in him, Ritsu chooses to lie to his friends that Uta is his girlfriend in order to be able to find excuse to spend more time at home with his sister (helped by the fact that they don't attend the same school, and thus none of their friends know their actual relationship. Ritsu's friends only know that he has a sister, and Uta's friends likewise), all the while pretending they're lovers outside of their home. However, their pretend lover relationship [[RomanticFakeRealTurn eventually turns into a geniune romance]], though they choose to try their best to not show it at home in fear of their parents finding out.
160* ''Literature/SisterPrincess'' and its "sequel" series, ''Anime/SisterPrincessRepure'', both have incestuous subtexts, but the subtext surfaces clearly in ''Repure'': in the very first episode, Karen recounts how she decided that she would be her brother's bride, despite society's disapproval of such a thing. In the original series it is less visible, although still present -- one sister (Chikage) was [[spoiler:Wataru's lover in a [[{{Reincarnation}} previous life]]]], Sakuya openly flirts with him (Rin Rin and Mamoru are more subtle hiding their flirting under the disguise of admiration for their Brother), and all twelve spend one episode making mock wedding dresses so they can play at being his "bride".
161* Aono in ''Manga/{{Sola}}'' is obsessed with her (younger, for a change) brother Yorito [[spoiler:to the point of creating a copy of him that took enough power to leave her bed-ridden for years]].
162* In ''Anime/TheSoulTaker'', Runa wanted to marry her twin brother Kyōsuke, who declined the honor.
163* The ''Manga/{{Spiral}}'' manga has a variation: Ayumu was in love with his sister-in-law even before [[TheMinnesotaFats his brother]] married her, and lived with her after he disappeared. Also, [[spoiler:since all of the Blade Children have the same father, Kousuke and Ryoko's relationship, despite growing up as childhood friends rather than siblings, is incestuous. Kousuke specifically asks Ryoko not to call him 'big brother,' saying that he wants to be able to dream of being with her]].
164* ''Swing Out Sisters'': Yuuta's two older sisters have the hots for him, and he starts intense sexual affairs with both. The sisters agree to share their brother, and find that they enjoy each other's bodies as well, thus forming a stable sibling love triangle.
165* ''Tayu-Tayu'': In Chapter 6, Reiju happens to overhear someone having sex inside an abandoned watermill and decides to see who it is. He's surprised to find it's two of his classmates: Haruka and ''[[{{Twincest}} her younger twin sister]]'', Sayaka. After the girls hogtie him, Haruka explains that she and her sister had been having sex with each other ever since all the boys their age had moved away. [[SituationalSexuality So the twins had no other options for relieving their pent-up hormones]]. Haruka adds that she and Sayaka soon became [[SingleTargetSexuality addicted to each other's bodies]], until it finally became impossible for them to be in the same room without tearing each others clothes off.
166* The original OAV series of ''Anime/TenchiMuyo'' is '''filled''' with incestual attractions -- the whole mess basically got started when Ayeka fell in love with and was engaged to marry her elder half-brother Katsuhito/Yosho.
167* Ren and Mihato in ''VisualNovel/TheyAreMyNobleMasters'' have a mutual brocon-siscon relationship, and are quite supportive of each other. It's almost sweet if one is willing to look past the sibling thing. Then again, this is a show that's filled to the brim with fetishes. They do have a route together in the original h-game.
168** In the same series, Shinra is very open about her sexual attraction towards Miyu, her younger sister. Worse, she sexually assaults her on numerous occasions, which is [[BlackComedyRape played entirely for laughs]]. That Miyu seems to be very ambiguous about this and that the youngest sister is actually jealous for being left out just adds to the {{squick}}-factor. Fetishes, indeed.
169* In ''Manga/TokyoGhoul :re'', [[spoiler:[[BigBad Nimura Furuta]]]] states that he has been in love with Rize since they were children, and intends to [[AndNowYouMustMarryMe marry her]] once his plans come to fruition. In fact, his decision to nearly kill her and allow [[spoiler:[[MadScientist Dr. Kanou]]]] to imprison and experiment on her was because he couldn't stand the thought of her having children with anyone else. It becomes even creepier when one examines the family tree in the background, because [[spoiler:[[GreaterScopeVillain Tsuneyoshi Washuu]]]] fathered ''all'' of the children in that generation. That makes Rize his '''half-sister''', in a family that routinely practices RoyalInbreeding.
170* ''Manga/ToLoveRu'' seems to heading this way, if Momo gets her way. Played for laughs when Mikan trying to resist the effects of [[LovePotion Celine's ]][[LoveIsInTheAir pollen.]]
171* Miyuki Takamachi in ''VideoGame/TriangleHeart3SweetSongsForever'' is actually Kyoya's [[KissingCousins cousin]], but adopted as his sister. In the game she's an option, though in {{canon}} he chooses to be with his PatientChildhoodLoveInterest, Shinobu. Yes, this shows up in early episodes ''Anime/MagicalGirlLyricalNanoha'': watch as Kyoya states he's going to visit Shinobu. For ''one second'' she shows jealousy, and then it's never mentioned again.
172** She seemed more annoyed at his using accompanying Nanoha to visit Suzuka as an excuse to see Shinobu, given that he doesn't seem completely open about his relationship (In A's episode 9, she teases him about being able to work with Shinobu for Christmas with a cute smile on her face). Interestingly enough, Miyuki calls him "Kyou-chan" (like she did in Triangle Heart) instead of "Onii-chan", like Nanoha does, which may indicate that they're NotBloodSiblings.
173* In ''Anime/TrueTears'' what starts as a case of BigBrotherInstinct evolves into borderline incest when we learn that Jun has romantic feelings toward Noe, his younger sister. This trope is also averted [[spoiler: at one point Shinchiro and Hiromi are under the impression that they are siblings but it turns out that they are [[NotBloodSiblings not related]]]].
174* It's revealed at the end of the oneshot manga ''Up And Down'' by Nishi Uko that the unnamed woman the protagonist pines for was in love with her sister. Her sister died so she [[HappilyAdopted adopted]] her niece. The woman states that she isn't sure if it's a blessing or if it's some sort of divine punishment because [[StrongFamilyResemblance her niece looks a lot like her mother.]]
175* In ''Manga/UruseiYatsura'', the Mizunokoji siblings have a one-sided version of this going on... [[SubvertedTrope more or less]]. According to their screwed up traditions, youngest daughter Asuka was raised in total isolation from the opposite sex, and with the terrible job her maids did in regards explaining the other sex (something worsened by her fact her first encounter with a guy was [[LovableSexManiac Ataru Moroboshi]]), [[DoesNotLikeMen she's terrified of men]]... except for "Big Brothers", whom her maids made out as a special, dependable, trustworthy exception to men in general. Between her phobia and her naive ignorance, she not only has a crush on her older brother Tobimaro, but is constantly trying to perform deeds with him that are only appropriate between lovers. Tobimaro, meanwhile, is perfectly aware of their relationship and that the things she tries are ''not'' appropriate, thus he freaks out when the poor girl comes on him. The fact that their crazy mother blames Asuka's actions on him, despite the fact she was the one who arranged for Asuka to be raised the way she was, only makes things more galling for him. The fact Asuka also has SuperStrength and is thus prone to crushing his bones whenever she gets frightened or romantic does ''not'' help.
176* ''Manga/VampireGame'': It's a big, feuding RoyallyScrewedUp family, and brother-sister incest is the ''least'' of their problems -- that couple looks downright ''normal'' in comparison, LoveMakesYouCrazy moments aside.
177* ''Manga/VampireKnight'': [[spoiler: Yuki, Kaname's adopted sister, becomes involved in a romantic relationship with him; this wouldn't normally count, except for TheReveal that they are biologically related and this doesn't stop the romance.]] Even more so, pureblood vampires like them actually encourage incestuous relationships, [[spoiler: and Yuuki's parents Haruka and Juri ''were siblings too''. (And their brother Ridou wanted Juri as his wife as well, [[ReplacementGoldfish which causes him to fixate on Yuuki]]...)]]
178* Lola and Layla from ''Manga/VenusVersusVirus'' have implications of a Sister-Sister version of this. Lola seems to not recirpocate her obsessed twin sisters feelings though.
179* In the first episode of the ''VideoGame/VoltageFighterGowcaizer'' OVA, the Villains Of The Week were an incestuous set of twins (who were empowered by the BigBad to save the dying sister) who merged into a gestalt monster "powered by love".
180* ''[[HGame Words Worth's]]''
181** Near the end of the first episode, Astral stumbles into [[LadyOfBlackMagic Maria's]] cell and loses consciousness. When he awakes moments later and sees her state of undress[[note]]due to Stallion's [[AttemptedRape attempt to rape her]], mere moments prior[[/note]], he decides [[LegFocus he likes what he sees]] and rapes her. Though it isn't explored in the OVA adaptation, the source material heavily implies that Maria and Astral are the biological offspring of Pollux and his wife Maria -- who bequeathed her ring to her daughter of the same name. Meaning, Astral raped his biological sister [[SeparatedAtBirth without realizing it.]]
182** Following the timeskip, Maria now has an 18 year old daughter named [[IdenticalGrandson Miyu]], who's heavily implied to be [[ChildByRape the result of Astral having raped her.]] Astral[[note]]who's amnesiac by this time, due to taking the full brunt of Maria's [[LightEmUp Mystral]] [[KameHameHadoken Window]], which propelled him 20 years into the future and left him without his memory[[/note]] meets Miyu after saving her from a rampaging bear, while she was bathing in the forest, and ends up sleeping with her. That's right: he banged his own daughter/niece... without being aware of it. Again. Talk about [[RunningGag keepin' it]] [[StealthPun IN]] the family.
183* ''Wan-ichi'': a LoveTriangle between two brothers [[ParentalIncest and their father]].
184* Pretty much the fundamental appeal of ''VisualNovel/YosugaNoSora'' combining with {{Twincest}}. Both tropes are also [[{{Deconstruction}} deconstructed]]: Sora’s attraction to Haruko is due to her physical and emotional dependence on him, coupled with her psychological instability, and while Haruko’s feelings are mutual, he struggles with the morality of their relationship, knowing society won’t accept them. Their arc ends with the two moving overseas, hoping to finally live happily together.
185* A SurpriseIncest variation appears in ''Manga/{{Yuureitou}}''. Like [[EvenTheGuysWantHim just about everyone]], [[spoiler:Satoko]] is attracted to Tetsuo. As it turns out, Tetsuo is [[spoiler:Marube's]] son. This makes them siblings. It never goes anywhere as the feelings are unrequited. It also may be a NotBloodRelated situation because it's heavily implied that she is adopted.
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