These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
Foe Yay: The Riddler's attempts to get Batman's attention include making dinner for him (lobsters, no less). While wearing a "Kiss the Genius" apron.
"Funny Aneurysm" Moment: The first Annual features a humorous little short story showing how easy it is for Harley to drop everything to go back to the Joker's side. It becomes a lot less amusing after reading Mad Love.
Les Yay: In "Mad Love", after Harley's been thrown out a window by The Joker, Renee Montoya cradles Harley in her arms, with a worried look on her face. While there's nothing romantic about it at the time, considering that Renee later came out as a lesbian and Harley later became close with Poison Ivy (in a relationship sometimes hinted at, but never explicitly stated to be sexual) puts a whole new light on it years later.
Moral Event Horizon: Two-Face finally seems to be making a recovery into sanity and happiness...so the Joker goads him into thinking that Bruce and Mrs. Dent are having an affair, and even has Harley convince a tabloid that they're engaged. So of course Two-Face snaps.
The Woobie: Surprisingly, the Riddler. After escaping from Arkham in Gotham Adventures, he decides he'll hide in a penthouse and send Batman clues to other people's crimes to satisfy his compulsions without incriminating himself like he normally does, because he hates hates hates Arkham and never wants to go back. Unfortunately, all those clues come together to make a clue to the location of his hideout...and he did that subconsciously.