These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
"Rock Lobster" deserves a mention as well, but it didn't get nearly as much airplay.
Ear Worm: Their entire discography is this. No exceptions.
Epic Riff: "Rock Lobster", "Private Idaho", "Hallucinating Pluto", "Pump", and "Lava" are the best examples.
Epileptic Trees: There are a ton circulating around the Throw It In line "You're what?!/Tin roof...rusted" from "Love Shack." The prevailing theory (as pointed out by Pop Up Video) is that it's some obscure slang for "pregnant". However, the Love Shack did have a tin roof, and it was rusted.
"Give Me Back My Man" and "Ain't It A Shame" are both sung by Cindy lamenting the loss of a man...and became this when her brother (and B-52's guitarist) Ricky Wilson died in 1985. In fact, "Ain't It A Shame" was one of the last songs the band recorded with him, and Cindy had no idea he had AIDS at the time.
The band gained many young fans in the 16 year gap between Good Stuff and Funplex, many of whom were not born when the band started out or even during their Cosmic Thing heyday. This meant that the release of Funplex was an occasion when many fans finally got to buy an album of new material when it came out.
Signature Song: Both "Rock Lobster" and "Love Shack". They have played both at every show since they were introduced and the success of them as singles was enough to sustain their entire career. To a lesser extent, they have also played "Planet Claire" and "Dance This Mess Around" at very nearly every show too, and the same with "Roam" later on.
What Do You Mean, It Wasn't Made on Drugs?: Although the band members did smoke marijuana in the early days (in one instance, leading to the writing of Quiche Lorraine), for many years their strongest vice has been alcohol, and they have never used psychedelics. The crazy ideas they come up with are inspired by weird movies and the band's sense of humor.
Alcohol is actually what led to the band forming, after they shared a large cocktail (Flaming Volcano) at a Chinese Restaurant and went to a mutual friend's house and started jamming on his music equipment, creating a song called "Killer Bees" (never recorded). They've written about alcohol in Deadbeat Club (99c Beer), Deviant Ingredient (Martini) and Is That You Mo-Dean? (Wine).