Nightmare Fuel: Gidney and Cloyd's first appearance in the pilot episode was a bit spooky, with the two fading in and out Cheshire Cat-style with creepy early '60s "spacey" sound effects. The limited animation actually made the scene eerier.
Boris looked pretty menacing in his first few appearances.
Also in the Pottsylvania Creeper story is the Pottsylvania Creeper, a plant that eats everything in sight, including people. Then its vines form a rocket that explodes in the air, which produces more Pottsylvania Creeper seeds. At the end of the story, we never see the people it ate again.
Since Boris, Natasha and Fearless Leader also get eaten at the end, this should be Fridge Logic since they show up alive and well in future stories.
Viewer Gender Confusion: June Foray's voice for Rocky is an all-time classic performance, but it sounds distinctively female despite the cartoon's insistence that Rocky is male.