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These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
YMMV: Glen or Glenda?
Big Lipped Alligator Moment: Every single time Bela Lugosi appears, he's sitting in a chair in a room full of horror-movie props while giving bizarre monologues with very little relation to the previous scene. During the notorious "pull the string!" moment, there's also Stock Footage of stampeding buffalo superimposed over Lugosi.
Reality Subtext: Aside from the obvious, Glen or Glenda? had Ed Wood doing a rare second take. In the first take of the scene, Dolores Fuller's character basically tossed the sweater at Glen with some hostility. Fuller wasn't exactly pleased with Wood at this point.
Rule-Abiding Rebel: Inverted. This movie tries to show that there is nothing disturbing about being a transvestite or transsexual — that they really could be rule-abiding. The strangeness of this film completely ruins the effect.