Please don't list this on a work's page as a trope. Examples can go on the work's YMMV tab.
Tearjerker: PB&J Otter
Done, believe it or not, on a show as overly child friendly as this. The Nothing Lasts Forever song from the episode Hope Castle. See it here. note Warning: prolonged repeated exposure to the song may result in depression, darkened soul, suicide urges and tantrum fits. You have been warned.