Please don't list this on a work's page as a trope. Examples can go on the work's YMMV tab.
Tear Jerker: Who Framed Roger Rabbit
Poor Marvin Acme...
Basically, anything having to do with the murder of Teddy Valiant and Eddie's memory of him. It's the heart of the film, after all, and is what transformed Eddie into a grumpy, alcoholic, washed-up has-been in the eyes of others. The only reason Roger comes to his aid at all is because he seemingly never realizes Eddie's changed, and is under the impression he still helps Toons.
The scene in the movie theater signals the start of the bond between Eddie and Roger. It's the first time we hear of how Teddy died... and foreshadows Judge Doom's real identity. Roger, heart-broken, and convinced that Eddie hates him and wants to kill him, may be hammy, but still tugs at the heart strings.
Roger crying, period. He's such a sweetheart. When he cries, our hearts ache.
Hell, even the scene of Eddie alone in his office, when the camera pans across the memorabilia of the brothers' successful cases.
The most heartbreaking scene is when Eddie is looking at his newly-developed vacation photos and smiling at all the pictures of him and Dolores. Only to look crushed when he finds one of Teddy.
The sweeping track that plays during these scenes, and kicks off the end credit sequence, is called "Eddie's Theme" for good reason.
In a meta example, Bob Hoskins, who portrayed Eddie Valiant, passed away at the age of 71. Now Eddie and his brother can be together again at last.
JUDGE DOOM DIPPING THE SHOE.
Especially because the shoe was so innocent and acted like a little defenseless puppy and good god the sounds it made when Judge Doom dipped it...
It was snuggling up next to his shoe! The poor little thing just wanted to be friends! (sob)
You know that there is another shoe out there that will always be alone now...
Seeing Roger sobbing desperately after he believes Jessica has cheated on him with Marvin Acme, especially when he pulls out pictures of their wedding and honeymoon. To see Roger, normally so cheerfully wacky, obviously completely heartbroken really hits you….
YMMV, but Wheezy's death. As he's dying, his angelic soul floats from his body. He desperately tries to grab it back, but it flutters off, and he dies.
In a meta way, Eddie's line, "Who needs a car in LA? We've got the best public transport system in the world," when you compare it to the gridlock of today and understand that the auto industry deliberately dismantled LA's streetcar system to force people to buy cars.
Jessica's line about how difficult it is to go around looking the way she does. If the hooting in the nightclub is any evidence, she doesn't get much respect.
Toontown is home to all toons, no matter the animation studio. If Judge Doom had succeeded, Snow White, Mickey, Donald Duck, and every other Disney character kids love—-along with the likes of characters such as Bugs Bunny and Daffy Duck—-would be dead forever.
Even though he was a greedy, manipulative Jerkass, R.K. Maroon did care for Toons to some degree. The moment he passionately declared "I've been around Toons all my life, I didn't wanna see 'em destroyed", explaining why he wanted Acme's Will could make him a Jerk with a Heart of Gold.
The way he cries out when he's shot in the back, he'd thought Eddie shot him.
If you know Betty Boop's history in regards to her anthropomorphic dog boyfriend, (short explanation, her boyfriend Bimbo was changed to being her pet when the Hays Code deemed their relationship to be promotion bestiality) then the part where she is staring wistfully at Jessica as she comments on how lucky Jessica is might tug at your heart strings a little.