This is based on opinion. Please don't list it on a work's trope example list.
Tear Jerker / Unbreakable
The opening scene. A doctor arrives at a department store dressing room where Elijah's mother has just given birth to him. He is shocked and horrified when he inspects the endlessly crying baby, then instructs her colleagues to apprise the ambulance of complications. He can barely get the words out of his mouth when he tells the teary and mortified woman that her son's arms and legs were broken while inside her uterus.
After Elijah's disastrous attempt to pursue the man wearing camouflage outside the stadium, the bitterness in his eyes at the hospital afterwards speaks volumes; you can tell he truly wishes to do right and be like the heroes in his beloved comics, but due to his condition he is destined only ever to be the villain to David's hero.
At the end, when David learns that Elijah was responsible for the disasters he's been mentioning throughout the film and as he tries to justify his actions. "You know what the scariest thing is? To not know your place in this world. To not know why you're here... That's... That's just an awful feeling."
Samuel L. Jackson's acting in that scene is extremely effective. Just the way he sounds like he's about to cry throughout the whole scene is sure to get the tear-ducts flowing.
Think of that scene from David's perspective too. He walked away from a potentially huge Football career because he felt it was his duty to help people and now he must live with the guilt that hundreds of innocent people died horribly because of him, even if he didn't know it.