Specially, two Washu centered scenes from the OAV. First, when she is petting Tenchi's little cousin, who calls her "mama", and she says "no, little child... I'm not your mama..." and hugs him. Second, when after the baby is taken back home, and Washu explains a part of her backstory to Tenchi and the girls - more specifically, about her husband and her estranged child. Yep, I know the second one dissolves in a Crowning Moment of Funny as Washu cheers herself up by showing off her adult form, but right before that... T_T
Ryouko is mortally wounded and keeps it a secret from Tenchi (even when hugging him goodbye), flies him into Jurai's defence zone, drops him off at the palace, but cannot go on due to blood loss. She pretends she won't go to rescue Aeka with Tenchi and leaves in her ship. Later she dies of blood loss in the cockpit with an odd smile... I shed manly tears.
The only one to see this is Ryo-Ohki. :(
Tenchi in Tokyo gave a very heartwrenching moment when Yugi remade Sakuya and trapped Tenchi in an illusionary world with her. Sakuya decided to let Tenchi go.
During the "Dark Washu" arc of the Shin Tenchi Muyo manga, the titular character gets Hijacked By Doctor Clay and becomes a villain, forcing Washu into a Sadistic Choice: brainwash the others into forgetting her and face her Evil Counterpart alone, or see them get repeatedly attacked. Washu chooses to wipe the others' memory, despite Tenchi being right there when she makes the decision and begging her not to go through with it.