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• Sakuya's reaction when she sees Tenchi after she got Stood Up by him, and she sheds tears despite trying not to:
  Sakuya: Masaki-kun, you're an horrible liar.
• Sakuya's Heroic BSOD when she realizes that she's got no real memories of her own;
  Sakuya (in a Troubled Fetal Position on the floor of her apartment): T-Tenchi-kun... help me... please...
• Yugi's backstory, specially as she's imprisioned inside a dark tomb after almost destroying Jurai, crying and screaming because she's scared of the dark. Made worse when Empress Hinase, the one sealing her away, is shown crying too, since she hates the idea of doing this to Yugi but can't do anything else
• When Yugi confronts Sakuya and tells her who she really is. If you don't feel minimally sorry for her (regardless of your side in the Ship-to-Ship Combat) when she looks at Yugi in tears and whispers "No, no... Tenchi-kun, help me... NOOOOO!" right before she's absorbed back into her, or with the Really Dead Montage in the end of the episode, full of happy Sakuya scenes, I doubt you have a real soul.
• Sasami visting Yugi in the final scene is a tear jerker for this troper. 'Good night, Yugi. Sleep tight.'